##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AscI Asp718I
AspEI AvrII BcgI BciVI BfrI
BglII BlnI BsaBI BseAI BsiWI
BsmBI BspDI BspEI BsrDI Bst1107I
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoRV EcoT22I FseI HindIII
HpaI KpnI KspI MamI MroI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RsrII SacII SalI ScaI
SfiI SgrAI SnaBI SpeI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AocI 3237 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3756 AGCGCT AGC^GCT
AseI 2823 ATTAAT AT^TAAT
AsnI 2823 ATTAAT AT^TAAT
Asp700I 1971 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 235 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1657 CACGTG CAC^GTG
BclI 1961 TGATCA T^GATCA
BsaWI 2216 TCCGGT [AT]^CCGG[AT]
BsmI 4111 GCATTC GAATGC(1/-1)
BspHI 1964 TCATGA T^CATGA
BspLU11I 731 ACATGT A^CATGT
BssHII 185 GCGCGC G^CGCGC
BssSI 1417 CACGAG CACGAG(-5/-1)
BstBI 271 TTCGAA TT^CGAA
BstEII 3558 GGTCACC G^GT[ACGT]ACC
BstPI 3558 GGTCACC G^GT[ACGT]ACC
Bsu36I 3237 CCTGAGG CC^T[ACGT]AGG
Eco47III 3756 AGCGCT AGC^GCT
Eco81I 3237 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3558 GGTCACC G^GT[ACGT]ACC
FbaI 1961 TGATCA T^GATCA
MfeI 740 CAATTG C^AATTG
MluI 4 ACGCGT A^CGCGT
MunI 740 CAATTG C^AATTG
NaeI 67 GCCGGC GCC^GGC
NgoMIV 67 GCCGGC G^CCGGC
NspV 271 TTCGAA TT^CGAA
PflFI 235 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1657 CACGTG CAC^GTG
PmlI 1657 CACGTG CAC^GTG
PshBI 2823 ATTAAT AT^TAAT
RcaI 1964 TCATGA T^CATGA
SacI 760 GAGCTC GAGCT^C
SapI 1293 GCTCTTC GCTCTTC(1/4)
SbfI 3919 CCTGCAGG CCTGCA^GG
SexAI 1955 ACCAGGT A^CC[AT]GGT
SfuI 271 TTCGAA TT^CGAA
SphI 2730 GCATGC GCATG^C
Sse8387I 3919 CCTGCAGG CCTGCA^GG
Tth111I 235 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4284 TCTAGA T^CTAGA
XmnI 1971 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 4 ACGCGT A^C[GA][CT]GT
731 ACATGT
Alw44I 2518 GTGCAC G^TGCAC
2748 GTGCAC
ApaLI 2518 GTGCAC G^TGCAC
2748 GTGCAC
AviII 2626 TGCGCA TGC^GCA
2727 TGCGCA
BalI 606 TGGCCA TGG^CCA
1661 TGGCCA
BamHI 3297 GGATCC G^GATCC
3732 GGATCC
BbvCI 1244 CCTCAGC CCTCAGC(-5/-2)
2796 GCTGAGG
BsaAI 1504 CACGTA [CT]AC^GT[GA]
1657 CACGTG
BsaI 2535 GGTCTC GGTCTC(1/5)
3198 GGTCTC
BstXI 4032 CCAGGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4092 CCAGCCATGTGG
DraIII 340 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2973 CACCCAGTG
EcoRI 904 GAATTC G^AATTC
2122 GAATTC
FspI 2626 TGCGCA TGC^GCA
2727 TGCGCA
HincII 2323 GTCAAC GT[CT]^[GA]AC
3536 GTCAAC
HindII 2323 GTCAAC GT[CT]^[GA]AC
3536 GTCAAC
MluNI 606 TGGCCA TGG^CCA
1661 TGGCCA
MscI 606 TGGCCA TGG^CCA
1661 TGGCCA
MseI 2824 TTAA T^TAA
3398 TTAA
PshAI 2837 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4262 GACCCTTGTC
SmaI 72 CCCGGG CCC^GGG
124 CCCGGG
Tru9I 2824 TTAA T^TAA
3398 TTAA
XmaCI 72 CCCGGG C^CCGGG
124 CCCGGG
XmaI 72 CCCGGG C^CCGGG
124 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspBI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII