##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AflIII AgeI
Aor51HI ApaI AscI Asp718I AviII
AvrII BbrPI BbvCI BciVI BlnI
BsaAI BsiEI BsiWI BspDI BspLU11I
BsrBI BsrFI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoT14I FseI FspI
KpnI MluI NaeI NcoI NgoMIV
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PvuI SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
StyI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 350 TCCGGA T^CCGGA
AclI 2636 AACGTT AA^CGTT
AflII 2963 CTTAAG C^TTAAG
AseI 4565 ATTAAT AT^TAAT
AsnI 4565 ATTAAT AT^TAAT
Asp700I 483 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1951 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1606 CTCGAG C^[CT]CG[GA]G
BamHI 2180 GGATCC G^GATCC
BanII 137 GAGCTC G[GA]GC[CT]^C
BcgI 1598 GCAGTATTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 567 TGATCA T^GATCA
BfrI 2963 CTTAAG C^TTAAG
BglI 215 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4299 AGATCT A^GATCT
BlpI 3514 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3514 GCTCAGC GC^T[ACGT]AGC
BsaWI 350 TCCGGA [AT]^CCGG[AT]
BseAI 350 TCCGGA T^CCGGA
BsmBI 639 CGTCTC CGTCTC(1/5)
BsoBI 1606 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3721 TGTACA T^GTACA
BspEI 350 TCCGGA T^CCGGA
BsrDI 602 CATTGC GCAATG(2/0)
BsrGI 3721 TGTACA T^GTACA
CelII 3514 GCTCAGC GC^T[ACGT]AGC
CpoI 183 CGGACCG CG^G[AT]CCG
DraIII 2671 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 2202 GATATC GAT^ATC
FbaI 567 TGATCA T^GATCA
HpaI 2729 GTTAAC GTT^AAC
KspI 216 CCGCGG CCGC^GG
MroI 350 TCCGGA T^CCGGA
PaeR7I 1606 CTCGAG C^TCGAG
PflFI 1951 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3273 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4565 ATTAAT AT^TAAT
Psp1406I 2636 AACGTT AA^CGTT
RsrII 183 CGGACCG CG^G[AT]CCG
SacI 137 GAGCTC GAGCT^C
SacII 216 CCGCGG CCGC^GG
SphI 997 GCATGC GCATG^C
SspBI 3721 TGTACA T^GTACA
SwaI 3491 ATTTAAAT ATTT^AAAT
Tth111I 1951 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1606 CTCGAG C^TCGAG
XmnI 483 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 148 GGCGCC G[GA]^CG[CT]C
173 GGCGCC
AhdI 879 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2893 GACGTACTGTC
AocI 2322 CCTCAGG CC^T[ACGT]AGG
3189 CCTCAGG
AspEI 879 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2893 GACGTACTGTC
BanI 148 GGCGCC G^G[CT][GA]CC
173 GGCGCC
BbeI 148 GGCGCC GGCGC^C
173 GGCGCC
BpmI 2413 CTCCAG CTGGAG(16/14)
2904 CTCCAG
BsaHI 148 GGCGCC G[GA]^CG[CT]C
173 GGCGCC
BsaI 2711 GAGACC GGTCTC(1/5)
2455 GGTCTC
BsmI 1046 GAATGC GAATGC(1/-1)
2680 GAATGC
BssHII 70 GCGCGC G^CGCGC
72 GCGCGC
BstXI 1736 CCATCTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2692 CCATGTCAGTGG
Bsu36I 2322 CCTCAGG CC^T[ACGT]AGG
3189 CCTCAGG
DsaI 86 CCGTGG C^C[GA][CT]GG
216 CCGCGG
Eam1105I 879 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2893 GACGTACTGTC
Eco81I 2322 CCTCAGG CC^T[ACGT]AGG
3189 CCTCAGG
EcoNI 2847 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4582 CCTTTATTAGG
HgaI 96 GCGTC GACGC(5/10)
4475 GCGTC
Hin1I 148 GGCGCC G[GA]^CG[CT]C
173 GGCGCC
KasI 148 GGCGCC G^GCGCC
173 GGCGCC
MfeI 2271 CAATTG C^AATTG
4449 CAATTG
MunI 2271 CAATTG C^AATTG
4449 CAATTG
NarI 148 GGCGCC GG^CGCC
173 GGCGCC
NdeI 2509 CATATG CA^TATG
4149 CATATG
NheI 1112 GCTAGC G^CTAGC
1779 GCTAGC
NspI 997 GCATGC [GA]CATG^[CT]
3976 ACATGC
PvuII 2724 CAGCTG CAG^CTG
3579 CAGCTG
SfoI 148 GGCGCC GGC^GCC
173 GGCGCC
SspI 2014 AATATT AAT^ATT
4522 AATATT
XbaI 3305 TCTAGA T^CTAGA
3834 TCTAGA
XcmI 1691 CCAGAAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3739 CCAGCAGTTGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaII BalI BbsI BbvI
BcnI BfaI BmyI BpuAI BsaBI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRI EcoRII EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI PflMI PleI
PpuMI PstI RcaI RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII