##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI Alw44I AlwNI Aor51HI
ApaLI AscI Asp718I AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BfrI BglI BglII BlnI
BsaHI BsaI BsiEI BsiWI BsmBI
BsoBI BspMI BsrBI BssHII BssSI
BstAPI BstEII BstPI BstUI CpoI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I FauI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MluI MluNI MscI
MvnI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SwaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 2642 GTATAC GT^[AC][GT]AC
AccIII 2047 TCCGGA T^CCGGA
AflIII 2623 ACATGT A^C[GA][CT]GT
AhdI 3363 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3600 CCTGAGG CC^T[ACGT]AGG
ApaI 344 GGGCCC GGGCC^C
AseI 915 ATTAAT AT^TAAT
AsnI 915 ATTAAT AT^TAAT
AspEI 3363 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1756 GTGCTC G[AT]GC[AT]^C
AspI 1558 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 91 GCTGAGG CCTCAGC(-5/-2)
BcgI 1113 CGAGCTATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 791 GGATAC GTATCC(6/5)
BclI 3083 TGATCA T^GATCA
BlpI 2675 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2675 GCTCAGC GC^T[ACGT]AGC
BsaAI 144 CACGTA [CT]AC^GT[GA]
BsaWI 2047 TCCGGA [AT]^CCGG[AT]
BseAI 2047 TCCGGA T^CCGGA
BsiHKAI 1756 GTGCTC G[AT]GC[AT]^C
BsmI 1055 GAATGC GAATGC(1/-1)
BspDI 4159 ATCGAT AT^CGAT
BspEI 2047 TCCGGA T^CCGGA
BspLU11I 2623 ACATGT A^CATGT
Bst1107I 2642 GTATAC GTA^TAC
BstBI 634 TTCGAA TT^CGAA
BstXI 422 CCAACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2642 GTATAC GTA^TAC
Bsu36I 3600 CCTGAGG CC^T[ACGT]AGG
CelII 2675 GCTCAGC GC^T[ACGT]AGC
CfoI 4138 GCGC GCG^C
ClaI 4159 ATCGAT AT^CGAT
DraII 343 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1717 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1464 CGGCCA [CT]^GGCC[GA]
Eam1105I 3363 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3600 CCTGAGG CC^T[ACGT]AGG
EcoO109I 343 AGGGCCC [GA]G^G[ACGT]CC[CT]
FbaI 3083 TGATCA T^GATCA
HhaI 4138 GCGC GCG^C
HinP1I 4138 GCGC G^CGC
MfeI 3706 CAATTG C^AATTG
MroI 2047 TCCGGA T^CCGGA
MunI 3706 CAATTG C^AATTG
NdeI 612 CATATG CA^TATG
NheI 3344 GCTAGC G^CTAGC
NlaIV 344 GGGCCC GG[ACGT]^[ACGT]CC
NspV 634 TTCGAA TT^CGAA
PflFI 1558 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1716 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 915 ATTAAT AT^TAAT
ScaI 1978 AGTACT AGT^ACT
SfuI 634 TTCGAA TT^CGAA
StuI 2557 AGGCCT AGG^CCT
Tth111I 1558 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1716 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1604 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BanII 344 GGGCCC G[GA]GC[CT]^C
3582 GAGCCC
BcnI 29 CCCGG CC^[GC]GG
3395 CCGGG
BpmI 211 CTCCAG CTGGAG(16/14)
1483 CTGGAG
BsmAI 2368 GAGAC GTCTC(1/5)
68 GTCTC
BsmFI 1654 GGGAC GGGAC(10/14)
1824 GGGAC
Bsp1407I 1079 TGTACA T^GTACA
3981 TGTACA
BspHI 3274 TCATGA T^CATGA
3613 TCATGA
BsrFI 1462 ACCGGC [GA]^CCGG[CT]
2197 ACCGGC
BsrGI 1079 TGTACA T^GTACA
3981 TGTACA
BstNI 12 CCAGG CC^[AT]GG
860 CCTGG
Cfr10I 1462 ACCGGC [GA]^CCGG[CT]
2197 ACCGGC
DrdI 825 GACTCACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1231 GACTCAAATGTC
EcoRI 651 GAATTC G^AATTC
729 GAATTC
EcoRII 12 CCAGG ^CC[AT]GG
860 CCTGG
EcoT14I 685 CCATGG C^C[AT][AT]GG
3778 CCATGG
EcoT22I 1206 ATGCAT ATGCA^T
2248 ATGCAT
MspA1I 1480 CAGCTG C[AC]G^C[GT]G
2842 CAGCTG
MvaI 12 CCAGG CC^[AT]GG
860 CCTGG
NciI 29 CCCGG CC^[GC]GG
3395 CCGGG
NcoI 685 CCATGG C^CATGG
3778 CCATGG
NsiI 1206 ATGCAT ATGCA^T
2248 ATGCAT
PvuII 1480 CAGCTG CAG^CTG
2842 CAGCTG
RcaI 3274 TCATGA T^CATGA
3613 TCATGA
SphI 1625 GCATGC GCATG^C
3460 GCATGC
SspBI 1079 TGTACA T^GTACA
3981 TGTACA
StyI 685 CCATGG C^C[AT][AT]GG
3778 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbsI BbvI
BfaI BmyI BpuAI BsaBI BsaJI
BseRI BsgI BsiYI BslI Bsp1286I
BsrDI BsrI BssKI BstF5I BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EarI EcoRV Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspI
MwoI NdeII NlaIII NspI PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI