##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AvaI AviII BalI
BamHI BanI BbeI BbrPI BbvCI
BcnI BfrI BglI BlpI Bpu1102I
BsaAI BsaHI BsaI BsgI BsiWI
BsoBI BspDI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoT22I FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KpnI KspI MaeII MfeI MluI
MluNI MscI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI TaqI TthHB8I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 1298 GTCTAC GT^[AC][GT]AC
AccII 34 CGCG CG^CG
AccIII 22 TCCGGA T^CCGGA
Alw44I 1429 GTGCAC G^TGCAC
AlwNI 765 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1263 CCTCAGG CC^T[ACGT]AGG
ApaLI 1429 GTGCAC G^TGCAC
AspI 1810 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 26 GAGCCC G[GA]GC[CT]^C
BcgI 2040 CGAATGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1854 GGATAC GTATCC(6/5)
BclI 2008 TGATCA T^GATCA
BglII 716 AGATCT A^GATCT
BsaBI 2003 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 22 TCCGGA [AT]^CCGG[AT]
BseAI 22 TCCGGA T^CCGGA
BsiEI 14 CGGCCG CG[GA][CT]^CG
BspEI 22 TCCGGA T^CCGGA
BspHI 692 TCATGA T^CATGA
BspMI 1989 ACCTGC ACCTGC(4/8)
BsrDI 837 GCAATG GCAATG(2/0)
BstAPI 1893 GCACAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 34 CGCG CG^CG
Bsu36I 1263 CCTCAGG CC^T[ACGT]AGG
DsaI 11 CCACGG C^C[GA][CT]GG
EaeI 14 CGGCCG [CT]^GGCC[GA]
EagI 14 CGGCCG C^GGCCG
EclXI 14 CGGCCG C^GGCCG
Eco52I 14 CGGCCG C^GGCCG
Eco81I 1263 CCTCAGG CC^T[ACGT]AGG
EcoRV 2646 GATATC GAT^ATC
FauI 1512 CCCGC CCCGC(4/6)
FbaI 2008 TGATCA T^GATCA
HapII 23 CCGG C^CGG
HgaI 32 GACGC GACGC(5/10)
HindIII 2178 AAGCTT A^AGCTT
HpaI 2709 GTTAAC GTT^AAC
HpaII 23 CCGG C^CGG
MamI 2003 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 22 TCCGGA T^CCGGA
MslI 1164 CATAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1994 CAGCTG C[AC]G^C[GT]G
MspI 23 CCGG C^CGG
MvnI 34 CGCG CG^CG
NdeI 2831 CATATG CA^TATG
PflFI 1810 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1994 CAGCTG CAG^CTG
RcaI 692 TCATGA T^CATGA
SapI 1747 GAAGAGC GCTCTTC(1/4)
ScaI 1328 AGTACT AGT^ACT
SmlI 2660 CTCAAG C^T[CT][GA]AG
SphI 438 GCATGC GCATG^C
SwaI 2413 ATTTAAAT ATTT^AAAT
TfiI 942 GATTC G^A[AT]TC
Tth111I 1810 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2436 TCTAGA T^CTAGA
XcmI 318 CCAAATTCGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 1573 ACATGT A^C[GA][CT]GT
2455 ACATGT
AseI 1826 ATTAAT AT^TAAT
1958 ATTAAT
AsnI 1826 ATTAAT AT^TAAT
1958 ATTAAT
Asp700I 487 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
937 GAATGGATTC
AspHI 225 GTGCTC G[AT]GC[AT]^C
1429 GTGCAC
BbvI 1993 GCAGC GCAGC(8/12)
252 GCTGC
BsiHKAI 225 GTGCTC G[AT]GC[AT]^C
1429 GTGCAC
BsiYI 1175 CCTTGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1990 CCTGCAGCTGG
BslI 1175 CCTTGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1990 CCTGCAGCTGG
BsmBI 707 GAGACG CGTCTC(1/5)
863 GAGACG
BsmFI 1258 GGGAC GGGAC(10/14)
1198 GTCCC
BsmI 668 GAATGC GAATGC(1/-1)
2041 GAATGC
Bsp1407I 87 TGTACA T^GTACA
516 TGTACA
BspLU11I 1573 ACATGT A^CATGT
2455 ACATGT
BsrGI 87 TGTACA T^GTACA
516 TGTACA
BstYI 716 AGATCT [GA]^GATC[CT]
2398 GGATCT
DraII 1196 AGGTCCC [GA]G^G[ACGT]CC[CT]
1258 GGGACCC
DraIII 1423 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1894 CACAAAGTG
EcoO109I 1196 AGGTCCC [GA]G^G[ACGT]CC[CT]
1258 GGGACCC
EcoRI 205 GAATTC G^AATTC
487 GAATTC
HincII 905 GTCAAC GT[CT]^[GA]AC
2709 GTTAAC
HindII 905 GTCAAC GT[CT]^[GA]AC
2709 GTTAAC
MflI 716 AGATCT [GA]^GATC[CT]
2398 GGATCT
PpuMI 1196 AGGTCCC [GA]G^G[AT]CC[CT]
1258 GGGACCC
PshBI 1826 ATTAAT AT^TAAT
1958 ATTAAT
PstI 1582 CTGCAG CTGCA^G
1991 CTGCAG
SspBI 87 TGTACA T^GTACA
516 TGTACA
TseI 252 GCTGC G^C[AT]GC
1993 GCAGC
XhoII 716 AGATCT [GA]^GATC[CT]
2398 GGATCT
XmnI 487 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
937 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII AvrII BbsI BfaI
BlnI BmyI BpmI BpuAI BsaJI
BseRI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI Tru9I Tsp45I
Tsp509I TspRI