################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AvaI AviII BalI BamHI BanI BbeI BbrPI BbvCI BcnI BfrI BglI BlpI Bpu1102I BsaAI BsaHI BsaI BsgI BsiWI BsoBI BspDI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I KasI KpnI KspI MaeII MfeI MluI MluNI MscI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI TaqI TthHB8I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 1298 GTCTAC GT^[AC][GT]AC AccII 34 CGCG CG^CG AccIII 22 TCCGGA T^CCGGA Alw44I 1429 GTGCAC G^TGCAC AlwNI 765 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1263 CCTCAGG CC^T[ACGT]AGG ApaLI 1429 GTGCAC G^TGCAC AspI 1810 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 26 GAGCCC G[GA]GC[CT]^C BcgI 2040 CGAATGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1854 GGATAC GTATCC(6/5) BclI 2008 TGATCA T^GATCA BglII 716 AGATCT A^GATCT BsaBI 2003 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 22 TCCGGA [AT]^CCGG[AT] BseAI 22 TCCGGA T^CCGGA BsiEI 14 CGGCCG CG[GA][CT]^CG BspEI 22 TCCGGA T^CCGGA BspHI 692 TCATGA T^CATGA BspMI 1989 ACCTGC ACCTGC(4/8) BsrDI 837 GCAATG GCAATG(2/0) BstAPI 1893 GCACAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 34 CGCG CG^CG Bsu36I 1263 CCTCAGG CC^T[ACGT]AGG DsaI 11 CCACGG C^C[GA][CT]GG EaeI 14 CGGCCG [CT]^GGCC[GA] EagI 14 CGGCCG C^GGCCG EclXI 14 CGGCCG C^GGCCG Eco52I 14 CGGCCG C^GGCCG Eco81I 1263 CCTCAGG CC^T[ACGT]AGG EcoRV 2646 GATATC GAT^ATC FauI 1512 CCCGC CCCGC(4/6) FbaI 2008 TGATCA T^GATCA HapII 23 CCGG C^CGG HgaI 32 GACGC GACGC(5/10) HindIII 2178 AAGCTT A^AGCTT HpaI 2709 GTTAAC GTT^AAC HpaII 23 CCGG C^CGG MamI 2003 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 22 TCCGGA T^CCGGA MslI 1164 CATAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1994 CAGCTG C[AC]G^C[GT]G MspI 23 CCGG C^CGG MvnI 34 CGCG CG^CG NdeI 2831 CATATG CA^TATG PflFI 1810 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 1994 CAGCTG CAG^CTG RcaI 692 TCATGA T^CATGA SapI 1747 GAAGAGC GCTCTTC(1/4) ScaI 1328 AGTACT AGT^ACT SmlI 2660 CTCAAG C^T[CT][GA]AG SphI 438 GCATGC GCATG^C SwaI 2413 ATTTAAAT ATTT^AAAT TfiI 942 GATTC G^A[AT]TC Tth111I 1810 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2436 TCTAGA T^CTAGA XcmI 318 CCAAATTCGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 1573 ACATGT A^C[GA][CT]GT 2455 ACATGT AseI 1826 ATTAAT AT^TAAT 1958 ATTAAT AsnI 1826 ATTAAT AT^TAAT 1958 ATTAAT Asp700I 487 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 937 GAATGGATTC AspHI 225 GTGCTC G[AT]GC[AT]^C 1429 GTGCAC BbvI 1993 GCAGC GCAGC(8/12) 252 GCTGC BsiHKAI 225 GTGCTC G[AT]GC[AT]^C 1429 GTGCAC BsiYI 1175 CCTTGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1990 CCTGCAGCTGG BslI 1175 CCTTGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1990 CCTGCAGCTGG BsmBI 707 GAGACG CGTCTC(1/5) 863 GAGACG BsmFI 1258 GGGAC GGGAC(10/14) 1198 GTCCC BsmI 668 GAATGC GAATGC(1/-1) 2041 GAATGC Bsp1407I 87 TGTACA T^GTACA 516 TGTACA BspLU11I 1573 ACATGT A^CATGT 2455 ACATGT BsrGI 87 TGTACA T^GTACA 516 TGTACA BstYI 716 AGATCT [GA]^GATC[CT] 2398 GGATCT DraII 1196 AGGTCCC [GA]G^G[ACGT]CC[CT] 1258 GGGACCC DraIII 1423 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1894 CACAAAGTG EcoO109I 1196 AGGTCCC [GA]G^G[ACGT]CC[CT] 1258 GGGACCC EcoRI 205 GAATTC G^AATTC 487 GAATTC HincII 905 GTCAAC GT[CT]^[GA]AC 2709 GTTAAC HindII 905 GTCAAC GT[CT]^[GA]AC 2709 GTTAAC MflI 716 AGATCT [GA]^GATC[CT] 2398 GGATCT PpuMI 1196 AGGTCCC [GA]G^G[AT]CC[CT] 1258 GGGACCC PshBI 1826 ATTAAT AT^TAAT 1958 ATTAAT PstI 1582 CTGCAG CTGCA^G 1991 CTGCAG SspBI 87 TGTACA T^GTACA 516 TGTACA TseI 252 GCTGC G^C[AT]GC 1993 GCAGC XhoII 716 AGATCT [GA]^GATC[CT] 2398 GGATCT XmnI 487 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 937 GAATGGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII AvrII BbsI BfaI BlnI BmyI BpmI BpuAI BsaJI BseRI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI Tru9I Tsp45I Tsp509I TspRI