##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspI BamHI BbeI BbrPI
BcnI BglI BglII BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BstAPI BstBI
BstEII BstPI BstUI BstYI CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FseI HaeII HgaI Hin1I HincII
HindII HpaI KasI KspI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1122 GGTACC G^GTACC
AflII 162 CTTAAG C^TTAAG
Asp700I 2097 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1122 GGTACC G^GTACC
AviII 2616 TGCGCA TGC^GCA
AvrII 1734 CCTAGG C^CTAGG
BalI 1082 TGGCCA TGG^CCA
BanI 1122 GGTACC G^G[CT][GA]CC
BbvCI 1399 GCTGAGG CCTCAGC(-5/-2)
BcgI 74 GCACGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3144 GGATAC GTATCC(6/5)
BclI 2490 TGATCA T^GATCA
BfrI 162 CTTAAG C^TTAAG
BlnI 1734 CCTAGG C^CTAGG
BsaBI 2789 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1514 GGTCTC GGTCTC(1/5)
BsmBI 1026 GAGACG CGTCTC(1/5)
BsmI 2619 GCATTC GAATGC(1/-1)
BspHI 150 TCATGA T^CATGA
BspMI 526 ACCTGC ACCTGC(4/8)
BssSI 75 CACGAG CACGAG(-5/-1)
Bst1107I 1048 GTATAC GTA^TAC
BstXI 797 CCAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1048 GTATAC GTA^TAC
CfoI 2617 GCGC GCG^C
EaeI 1082 TGGCCA [CT]^GGCC[GA]
EarI 2930 CTCTTC CTCTTC(1/4)
FauI 34 CCCGC CCCGC(4/6)
FbaI 2490 TGATCA T^GATCA
FspI 2616 TGCGCA TGC^GCA
HapII 692 CCGG C^CGG
HhaI 2617 GCGC GCG^C
HinP1I 2617 GCGC G^CGC
HpaII 692 CCGG C^CGG
KpnI 1122 GGTACC GGTAC^C
Ksp632I 2930 CTCTTC CTCTTC(1/4)
MaeII 1029 ACGT A^CGT
MamI 2789 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1082 TGGCCA TGG^CCA
MscI 1082 TGGCCA TGG^CCA
MspI 692 CCGG C^CGG
NdeI 2984 CATATG CA^TATG
NspI 767 GCATGC [GA]CATG^[CT]
PstI 845 CTGCAG CTGCA^G
RcaI 150 TCATGA T^CATGA
SacI 1955 GAGCTC GAGCT^C
SexAI 1118 ACCTGGT A^CC[AT]GGT
SphI 767 GCATGC GCATG^C
StuI 2472 AGGCCT AGG^CCT
XmnI 2097 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 80 GGATC GGATC(4/5)
1584 GGATC
AlwNI 1060 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1114 CAGGACCTG
AocI 924 CCTGAGG CC^T[ACGT]AGG
2382 CCTTAGG
AvaI 1552 CCCGAG C^[CT]CG[GA]G
2468 CCCGAG
BsmFI 45 GGGAC GGGAC(10/14)
1766 GGGAC
BsoBI 1552 CCCGAG C^[CT]CG[GA]G
2468 CCCGAG
Bsp1407I 1297 TGTACA T^GTACA
1994 TGTACA
BsrGI 1297 TGTACA T^GTACA
1994 TGTACA
Bsu36I 924 CCTGAGG CC^T[ACGT]AGG
2382 CCTTAGG
Eco81I 924 CCTGAGG CC^T[ACGT]AGG
2382 CCTTAGG
EcoRV 809 GATATC GAT^ATC
1204 GATATC
PflMI 531 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCAAGGAATGG
PvuII 263 CAGCTG CAG^CTG
1802 CAGCTG
ScaI 1182 AGTACT AGT^ACT
3019 AGTACT
SmlI 162 CTTAAG C^T[CT][GA]AG
1964 CTCAAG
SspBI 1297 TGTACA T^GTACA
1994 TGTACA
SspI 2717 AATATT AAT^ATT
2949 AATATT
Van91I 531 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCAAGGAATGG
XbaI 2165 TCTAGA T^CTAGA
2300 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I