##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BbvCI
BclI BfrI BglII BlnI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BsrDI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MluI MroI NdeI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Asp700I 2154 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 2750 CACGTG CAC^GTG
BlpI 2398 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2398 GCTGAGC GC^T[ACGT]AGC
BsaAI 2750 CACGTG [CT]AC^GT[GA]
BsaWI 69 ACCGGA [AT]^CCGG[AT]
BsiEI 1610 CGGCCG CG[GA][CT]^CG
Bsp1407I 2844 TGTACA T^GTACA
BsrGI 2844 TGTACA T^GTACA
BstBI 1769 TTCGAA TT^CGAA
BstXI 1817 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2398 GCTGAGC GC^T[ACGT]AGC
DraIII 708 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3070 GACTAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1610 CGGCCG C^GGCCG
EclXI 1610 CGGCCG C^GGCCG
Eco52I 1610 CGGCCG C^GGCCG
HincII 1204 GTCAAC GT[CT]^[GA]AC
HindII 1204 GTCAAC GT[CT]^[GA]AC
MfeI 2997 CAATTG C^AATTG
MunI 2997 CAATTG C^AATTG
NcoI 3008 CCATGG C^CATGG
NheI 2374 GCTAGC G^CTAGC
NspV 1769 TTCGAA TT^CGAA
PmaCI 2750 CACGTG CAC^GTG
PmlI 2750 CACGTG CAC^GTG
PvuII 2591 CAGCTG CAG^CTG
ScaI 2662 AGTACT AGT^ACT
SfuI 1769 TTCGAA TT^CGAA
SgrAI 1072 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 2441 CTTGAG C^T[CT][GA]AG
SspBI 2844 TGTACA T^GTACA
XmnI 2154 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 1919 ACATGT A^C[GA][CT]GT
2719 ACATGT
AhdI 805 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
874 GACCCGTCGTC
AocI 565 CCTGAGG CC^T[ACGT]AGG
2884 CCTTAGG
AspEI 805 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
874 GACCCGTCGTC
BamHI 2341 GGATCC G^GATCC
2816 GGATCC
BcgI 819 GCACCAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
893 GCAAGGGCATCG
BciVI 1595 GGATAC GTATCC(6/5)
2419 GTATCC
BsaBI 400 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2817 GATCCCCATC
BsaI 2692 GAGACC GGTCTC(1/5)
428 GGTCTC
BsgI 1654 CTGCAC GTGCAG(16/14)
1812 CTGCAC
BspLU11I 1919 ACATGT A^CATGT
2719 ACATGT
BsrBI 1114 CCGCTC CCGCTC(-3/-3)
3063 GAGCGG
Bsu36I 565 CCTGAGG CC^T[ACGT]AGG
2884 CCTTAGG
DraI 3295 TTTAAA TTT^AAA
3451 TTTAAA
Eam1105I 805 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
874 GACCCGTCGTC
Eco81I 565 CCTGAGG CC^T[ACGT]AGG
2884 CCTTAGG
MamI 400 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2817 GATCCCCATC
NaeI 1073 GCCGGC GCC^GGC
1372 GCCGGC
NgoMIV 1073 GCCGGC G^CCGGC
1372 GCCGGC
SacI 1888 GAGCTC GAGCT^C
2401 GAGCTC
SfiI 1897 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1906 GGCCGCTGTGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI