##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
Asp718I AviII AvrII BamHI BbrPI
BbvCI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstEII BstPI BstZ17I CelII
ClaI CpoI DrdI EcoNI EcoO65I
FseI FspI HpaI KpnI KspI
MfeI MluI MroI MunI NheI
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SwaI
Van91I XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 23 CGCG CG^CG
AcyI 123 GGCGCC G[GA]^CG[CT]C
AflIII 1673 ACATGT A^C[GA][CT]GT
AhdI 403 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 754 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2706 CCTAAGG CC^T[ACGT]AGG
ApaI 140 GGGCCC GGGCC^C
AspEI 403 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 792 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 136 CCCGGG C^[CT]CG[GA]G
BbeI 123 GGCGCC GGCGC^C
BciVI 890 GGATAC GTATCC(6/5)
BsaBI 1715 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 123 GGCGCC G[GA]^CG[CT]C
BsaI 2490 GAGACC GGTCTC(1/5)
BsgI 1050 GTGCAG GTGCAG(16/14)
BsiEI 148 CGGCCG CG[GA][CT]^CG
BsoBI 136 CCCGGG C^[CT]CG[GA]G
BspHI 185 TCATGA T^CATGA
BspLU11I 1673 ACATGT A^CATGT
BspMI 1892 ACCTGC ACCTGC(4/8)
BstAPI 639 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2025 TTCGAA TT^CGAA
BstUI 23 CGCG CG^CG
Bsu36I 2706 CCTAAGG CC^T[ACGT]AGG
DraIII 936 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2875 CCATGG C^C[GA][CT]GG
EagI 148 CGGCCG C^GGCCG
Eam1105I 403 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 148 CGGCCG C^GGCCG
Eco52I 148 CGGCCG C^GGCCG
Eco81I 2706 CCTAAGG CC^T[ACGT]AGG
EcoRV 713 GATATC GAT^ATC
EcoT22I 220 ATGCAT ATGCA^T
HgaI 446 GCGTC GACGC(5/10)
Hin1I 123 GGCGCC G[GA]^CG[CT]C
HincII 1118 GTTGAC GT[CT]^[GA]AC
HindII 1118 GTTGAC GT[CT]^[GA]AC
HindIII 2162 AAGCTT A^AGCTT
KasI 123 GGCGCC G^GCGCC
MaeII 1703 ACGT A^CGT
MamI 1715 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 23 CGCG CG^CG
NarI 123 GGCGCC GG^CGCC
NcoI 2875 CCATGG C^CATGG
NsiI 220 ATGCAT ATGCA^T
NspV 2025 TTCGAA TT^CGAA
PflFI 792 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2558 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 185 TCATGA T^CATGA
SacI 1941 GAGCTC GAGCT^C
SapI 1484 GAAGAGC GCTCTTC(1/4)
ScaI 2528 AGTACT AGT^ACT
SfoI 123 GGCGCC GGC^GCC
SfuI 2025 TTCGAA TT^CGAA
SmaI 136 CCCGGG CCC^GGG
TaqI 2026 TCGA T^CGA
Tth111I 792 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2026 TCGA T^CGA
XmaCI 136 CCCGGG C^CCGGG
XmaI 136 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Aor51HI 4 AGCGCT AGC^GCT
44 AGCGCT
AseI 741 ATTAAT AT^TAAT
1455 ATTAAT
AsnI 741 ATTAAT AT^TAAT
1455 ATTAAT
AspHI 1126 GTGCTC G[AT]GC[AT]^C
1941 GAGCTC
BalI 707 TGGCCA TGG^CCA
1333 TGGCCA
BcnI 136 CCCGG CC^[GC]GG
137 CCGGG
BpmI 315 CTGGAG CTGGAG(16/14)
1564 CTGGAG
BseRI 66 CTCCTC GAGGAG(10/8)
1040 CTCCTC
BsiHKAI 1126 GTGCTC G[AT]GC[AT]^C
1941 GAGCTC
BsmBI 114 CGTCTC CGTCTC(1/5)
447 CGTCTC
BsmFI 660 GTCCC GGGAC(10/14)
2334 GTCCC
BsmI 320 GAATGC GAATGC(1/-1)
388 GAATGC
Bsp1407I 1277 TGTACA T^GTACA
2550 TGTACA
BsrDI 800 CATTGC GCAATG(2/0)
2458 GCAATG
BsrGI 1277 TGTACA T^GTACA
2550 TGTACA
BssSI 117 CTCGTG CACGAG(-5/-1)
1618 CTCGTG
BstXI 710 CCAGATATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1724 CCAGTCTTCTGG
DraI 507 TTTAAA TTT^AAA
2799 TTTAAA
Eco47III 4 AGCGCT AGC^GCT
44 AGCGCT
EcoRI 1603 GAATTC G^AATTC
1875 GAATTC
FauI 154 CCCGC CCCGC(4/6)
212 GCGGG
MluNI 707 TGGCCA TGG^CCA
1333 TGGCCA
MscI 707 TGGCCA TGG^CCA
1333 TGGCCA
MslI 1379 CACGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2454 CACAGCAATG
NciI 136 CCCGG CC^[GC]GG
137 CCGGG
NdeI 2156 CATATG CA^TATG
2497 CATATG
PleI 678 GACTC GAGTC(4/5)
572 GAGTC
PshBI 741 ATTAAT AT^TAAT
1455 ATTAAT
PstI 103 CTGCAG CTGCA^G
613 CTGCAG
PvuII 754 CAGCTG CAG^CTG
2354 CAGCTG
SfcI 103 CTGCAG C^T[GA][CT]AG
613 CTGCAG
SspBI 1277 TGTACA T^GTACA
2550 TGTACA
SspI 1977 AATATT AAT^ATT
2324 AATATT
StuI 922 AGGCCT AGG^CCT
2572 AGGCCT
XcmI 271 CCAACTCCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1724 CCAGTCTTCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanI BanII
BbsI BbvI BclI BfaI BglII
BmyI BpuAI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NaeI
NdeII NgoMIV NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XbaI XhoII XmnI