##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BcnI BglI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KspI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1636 GGTACC G^GTACC
AciI 100 CCGC CCGC(-3/-1)
AflIII 1932 ACATGT A^C[GA][CT]GT
ApaI 1571 GGGCCC GGGCC^C
Asp718I 1636 GGTACC G^GTACC
AspI 1115 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 825 GGTCC G^G[AT]CC
BalI 1698 TGGCCA TGG^CCA
BanI 1636 GGTACC G^G[CT][GA]CC
BbsI 954 GTCTTC GAAGAC(2/6)
BglII 695 AGATCT A^GATCT
BpuAI 954 GTCTTC GAAGAC(2/6)
BsaI 805 GGTCTC GGTCTC(1/5)
BseRI 1796 GAGGAG GAGGAG(10/8)
BspLU11I 1932 ACATGT A^CATGT
BstAPI 102 GCATTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 410 TTCGAA TT^CGAA
DraII 1571 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 1698 TGGCCA [CT]^GGCC[GA]
EcoNI 309 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1571 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 81 CCTTGG C^C[AT][AT]GG
HgaI 402 GCGTC GACGC(5/10)
KpnI 1636 GGTACC GGTAC^C
MaeII 952 ACGT A^CGT
MfeI 2020 CAATTG C^AATTG
MluNI 1698 TGGCCA TGG^CCA
MscI 1698 TGGCCA TGG^CCA
MslI 1759 CATAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1017 CAGCTG C[AC]G^C[GT]G
MunI 2020 CAATTG C^AATTG
NspI 1932 ACATGT [GA]CATG^[CT]
NspV 410 TTCGAA TT^CGAA
PflFI 1115 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1939 CCACATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1627 GAGTC GAGTC(4/5)
PvuII 1017 CAGCTG CAG^CTG
SacI 1719 GAGCTC GAGCT^C
SfcI 699 CTACAG C^T[GA][CT]AG
SfuI 410 TTCGAA TT^CGAA
SspI 1532 AATATT AAT^ATT
StyI 81 CCTTGG C^C[AT][AT]GG
SwaI 1360 ATTTAAAT ATTT^AAAT
TfiI 882 GATTC G^A[AT]TC
Tth111I 1115 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1939 CCACATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1715 TCTAGA T^CTAGA
XcmI 706 CCAGTCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 1282 CTTAAG C^TTAAG
2097 CTTAAG
AlwI 97 GATCC GGATC(4/5)
2115 GGATC
Asp700I 667 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1329 GAATTCTTTC
BanII 1571 GGGCCC G[GA]GC[CT]^C
1719 GAGCTC
BbvCI 828 CCTCAGC CCTCAGC(-5/-2)
1793 GCTGAGG
BclI 1045 TGATCA T^GATCA
1784 TGATCA
BfrI 1282 CTTAAG C^TTAAG
2097 CTTAAG
BlpI 1026 GCTGAGC GC^T[ACGT]AGC
2060 GCTCAGC
BpmI 260 CTCCAG CTGGAG(16/14)
905 CTCCAG
Bpu1102I 1026 GCTGAGC GC^T[ACGT]AGC
2060 GCTCAGC
BsmFI 1446 GGGAC GGGAC(10/14)
737 GTCCC
BstYI 96 AGATCC [GA]^GATC[CT]
695 AGATCT
CelII 1026 GCTGAGC GC^T[ACGT]AGC
2060 GCTCAGC
EcoRI 344 GAATTC G^AATTC
1329 GAATTC
FbaI 1045 TGATCA T^GATCA
1784 TGATCA
HinfI 882 GATTC G^A[ACGT]TC
1627 GAGTC
MflI 96 AGATCC [GA]^GATC[CT]
695 AGATCT
ScaI 1667 AGTACT AGT^ACT
1693 AGTACT
SmlI 1282 CTTAAG C^T[CT][GA]AG
2097 CTTAAG
XhoII 96 AGATCC [GA]^GATC[CT]
695 AGATCT
XmnI 667 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1329 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI AspHI BbvI BfaI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I