##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwNI AocI
Aor51HI AscI Asp718I AspEI AspI
AviII AvrII BbrPI BclI BfrI
BglI BlnI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FbaI FseI
FspI HpaI KpnI MluI MroI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I StuI TaqI Tth111I
TthHB8I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AcyI 286 GGCGCC G[GA]^CG[CT]C
Alw44I 2815 GTGCAC G^TGCAC
ApaLI 2815 GTGCAC G^TGCAC
AseI 2051 ATTAAT AT^TAAT
AsnI 2051 ATTAAT AT^TAAT
Asp700I 1404 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2620 GGATCC G^GATCC
BbeI 286 GGCGCC GGCGC^C
BbsI 553 GAAGAC GAAGAC(2/6)
BbvCI 15 CCTCAGC CCTCAGC(-5/-2)
BciVI 27 GTATCC GTATCC(6/5)
BglII 3196 AGATCT A^GATCT
BlpI 2115 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2115 GCTCAGC GC^T[ACGT]AGC
BpuAI 553 GAAGAC GAAGAC(2/6)
BsaBI 504 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 286 GGCGCC G[GA]^CG[CT]C
BsmFI 785 GTCCC GGGAC(10/14)
BsmI 2423 GAATGC GAATGC(1/-1)
Bsp1407I 2417 TGTACA T^GTACA
BspHI 3202 TCATGA T^CATGA
BsrDI 455 CATTGC GCAATG(2/0)
BsrFI 334 GCCGGC [GA]^CCGG[CT]
BsrGI 2417 TGTACA T^GTACA
BstAPI 463 GCAACCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2115 GCTCAGC GC^T[ACGT]AGC
Cfr10I 334 GCCGGC [GA]^CCGG[CT]
DsaI 127 CCGCGG C^C[GA][CT]GG
EcoRV 3230 GATATC GAT^ATC
HgaI 3025 GACGC GACGC(5/10)
Hin1I 286 GGCGCC G[GA]^CG[CT]C
HincII 1641 GTTGAC GT[CT]^[GA]AC
HindII 1641 GTTGAC GT[CT]^[GA]AC
HindIII 2550 AAGCTT A^AGCTT
KasI 286 GGCGCC G^GCGCC
KspI 127 CCGCGG CCGC^GG
MamI 504 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1709 CAATTG C^AATTG
MunI 1709 CAATTG C^AATTG
NaeI 334 GCCGGC GCC^GGC
NarI 286 GGCGCC GG^CGCC
NdeI 1319 CATATG CA^TATG
NgoMIV 334 GCCGGC G^CCGGC
PflMI 635 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 936 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 684 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2051 ATTAAT AT^TAAT
RcaI 3202 TCATGA T^CATGA
SacI 3000 GAGCTC GAGCT^C
SacII 127 CCGCGG CCGC^GG
SfoI 286 GGCGCC GGC^GCC
SmaI 48 CCCGGG CCC^GGG
SphI 1722 GCATGC GCATG^C
SspBI 2417 TGTACA T^GTACA
SwaI 3170 ATTTAAAT ATTT^AAAT
Van91I 635 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 48 CCCGGG C^CCGGG
XmaI 48 CCCGGG C^CCGGG
XmnI 1404 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
ApaI 132 GGGCCC GGGCC^C
212 GGGCCC
AvaI 48 CCCGGG C^[CT]CG[GA]G
217 CCCGAG
BalI 230 TGGCCA TGG^CCA
518 TGGCCA
BcgI 650 GCAATAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1471 GCACTTGCATCG
BsaI 2482 GAGACC GGTCTC(1/5)
1515 GGTCTC
BsoBI 48 CCCGGG C^[CT]CG[GA]G
217 CCCGAG
DraI 2661 TTTAAA TTT^AAA
3171 TTTAAA
EaeI 230 TGGCCA [CT]^GGCC[GA]
518 TGGCCA
EcoRI 667 GAATTC G^AATTC
1404 GAATTC
EcoT14I 814 CCAAGG C^C[AT][AT]GG
1396 CCAAGG
HaeII 40 AGCGCC [GA]GCGC^[CT]
286 GGCGCC
HphI 1936 GGTGA GGTGA(8/7)
2513 TCACC
MluNI 230 TGGCCA TGG^CCA
518 TGGCCA
MscI 230 TGGCCA TGG^CCA
518 TGGCCA
PstI 244 CTGCAG CTGCA^G
2854 CTGCAG
SmlI 2436 CTTGAG C^T[CT][GA]AG
2784 CTCAAG
StyI 814 CCAAGG C^C[AT][AT]GG
1396 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII