##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AvrII
BbsI BcgI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaWI BseAI
BsiWI BsmI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
HpaI KpnI KspI MfeI MluI
MroI MunI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I RcaI RsrII SacII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 175 GTAGAC GT^[AC][GT]AC
AccII 62 CGCG CG^CG
AcsI 1578 GAATTT [GA]^AATT[CT]
AhdI 2677 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 223 CCTGAGG CC^T[ACGT]AGG
ApaI 2230 GGGCCC GGGCC^C
ApoI 1578 GAATTT [GA]^AATT[CT]
AspEI 2677 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3369 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 896 TGGCCA TGG^CCA
BciVI 3066 GGATAC GTATCC(6/5)
BclI 1229 TGATCA T^GATCA
BsaI 1753 GAGACC GGTCTC(1/5)
BsiEI 1026 CGATCG CG[GA][CT]^CG
BsmBI 541 GAGACG CGTCTC(1/5)
Bsp1407I 2738 TGTACA T^GTACA
BspDI 415 ATCGAT AT^CGAT
BsrGI 2738 TGTACA T^GTACA
BstEII 3024 GGTAACC G^GT[ACGT]ACC
BstPI 3024 GGTAACC G^GT[ACGT]ACC
BstUI 62 CGCG CG^CG
Bsu36I 223 CCTGAGG CC^T[ACGT]AGG
ClaI 415 ATCGAT AT^CGAT
Eam1105I 2677 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 223 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3024 GGTAACC G^GT[ACGT]ACC
EcoT22I 1609 ATGCAT ATGCA^T
FbaI 1229 TGATCA T^GATCA
HindIII 1944 AAGCTT A^AGCTT
MluNI 896 TGGCCA TGG^CCA
MscI 896 TGGCCA TGG^CCA
MvnI 62 CGCG CG^CG
NaeI 2730 GCCGGC GCC^GGC
NgoMIV 2730 GCCGGC G^CCGGC
NheI 2908 GCTAGC G^CTAGC
NsiI 1609 ATGCAT ATGCA^T
PflFI 3369 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 1026 CGATCG CGAT^CG
SspBI 2738 TGTACA T^GTACA
Tsp509I 1579 AATT ^AATT
Tth111I 3369 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 396 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 1463 ACATGT A^C[GA][CT]GT
1772 ACATGT
AviII 677 TGCGCA TGC^GCA
2216 TGCGCA
BamHI 612 GGATCC G^GATCC
1713 GGATCC
BbeI 2354 GGCGCC GGCGC^C
2829 GGCGCC
BbrPI 787 CACGTG CAC^GTG
1516 CACGTG
BbvCI 1407 CCTCAGC CCTCAGC(-5/-2)
2581 GCTGAGG
BspLU11I 1463 ACATGT A^CATGT
1772 ACATGT
BsrBI 184 GAGCGG CCGCTC(-3/-3)
1330 GAGCGG
BsrFI 2730 GCCGGC [GA]^CCGG[CT]
3362 GCCGGT
BstAPI 588 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
843 GCAGATGGTGC
Cfr10I 2730 GCCGGC [GA]^CCGG[CT]
3362 GCCGGT
DraIII 2011 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2821 CACCAGGTG
FspI 677 TGCGCA TGC^GCA
2216 TGCGCA
HaeII 2354 GGCGCC [GA]GCGC^[CT]
2829 GGCGCC
HincII 343 GTTGAC GT[CT]^[GA]AC
2695 GTCAAC
HindII 343 GTTGAC GT[CT]^[GA]AC
2695 GTCAAC
KasI 2354 GGCGCC G^GCGCC
2829 GGCGCC
MseI 1082 TTAA T^TAA
1886 TTAA
NarI 2354 GGCGCC GG^CGCC
2829 GGCGCC
NcoI 312 CCATGG C^CATGG
2894 CCATGG
PmaCI 787 CACGTG CAC^GTG
1516 CACGTG
PmlI 787 CACGTG CAC^GTG
1516 CACGTG
PshAI 70 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3087 GACAGGTGTC
PvuII 592 CAGCTG CAG^CTG
1708 CAGCTG
SapI 181 GAAGAGC GCTCTTC(1/4)
217 GAAGAGC
SbfI 1392 CCTGCAGG CCTGCA^GG
3433 CCTGCAGG
SexAI 2012 ACCTGGT A^CC[AT]GGT
2822 ACCAGGT
SfoI 2354 GGCGCC GGC^GCC
2829 GGCGCC
SmaI 1631 CCCGGG CCC^GGG
2358 CCCGGG
Sse8387I 1392 CCTGCAGG CCTGCA^GG
3433 CCTGCAGG
Tru9I 1082 TTAA T^TAA
1886 TTAA
XmaCI 1631 CCCGGG C^CCGGG
2358 CCCGGG
XmaI 1631 CCCGGG C^CCGGG
2358 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaBI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII