################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BciVI BfrI BglI BlnI BsaAI BsaBI BseAI BsgI BsiEI BsiWI BsmI BspEI BspHI BspLU11I BsrBI BssHII BssSI BstBI BstEII BstPI Bsu36I CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FseI FspI KpnI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 211 CGCG CG^CG AcyI 237 GGCGCC G[GA]^CG[CT]C AflIII 2888 ACACGT A^C[GA][CT]GT AlwNI 3044 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 183 CCCGGG C^[CT]CG[GA]G BalI 1193 TGGCCA TGG^CCA BamHI 1997 GGATCC G^GATCC BbeI 237 GGCGCC GGCGC^C BbsI 2719 GTCTTC GAAGAC(2/6) BpuAI 2719 GTCTTC GAAGAC(2/6) BsaHI 237 GGCGCC G[GA]^CG[CT]C BsaWI 753 ACCGGA [AT]^CCGG[AT] BsmBI 1807 CGTCTC CGTCTC(1/5) BsoBI 183 CCCGGG C^[CT]CG[GA]G BspDI 1972 ATCGAT AT^CGAT BsrFI 258 GCCGGT [GA]^CCGG[CT] Bst1107I 3127 GTATAC GTA^TAC BstAPI 3043 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 211 CGCG CG^CG BstZ17I 3127 GTATAC GTA^TAC Cfr10I 258 GCCGGT [GA]^CCGG[CT] ClaI 1972 ATCGAT AT^CGAT DraI 2079 TTTAAA TTT^AAA DrdI 2043 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 486 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 237 GGCGCC G[GA]^CG[CT]C HpaI 514 GTTAAC GTT^AAC KasI 237 GGCGCC G^GCGCC MaeII 2890 ACGT A^CGT MluNI 1193 TGGCCA TGG^CCA MscI 1193 TGGCCA TGG^CCA MvnI 211 CGCG CG^CG NarI 237 GGCGCC GG^CGCC NcoI 332 CCATGG C^CATGG SapI 1162 GAAGAGC GCTCTTC(1/4) ScaI 109 AGTACT AGT^ACT SfoI 237 GGCGCC GGC^GCC SmaI 183 CCCGGG CCC^GGG SphI 1868 GCATGC GCATG^C StuI 434 AGGCCT AGG^CCT TfiI 2638 GAATC G^A[AT]TC XmaCI 183 CCCGGG C^CCGGG XmaI 183 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BclI 2689 TGATCA T^GATCA 3482 TGATCA BcnI 183 CCCGG CC^[GC]GG 184 CCGGG BsaI 377 GAGACC GGTCTC(1/5) 2387 GAGACC Bsp1407I 1515 TGTACA T^GTACA 2786 TGTACA BsrGI 1515 TGTACA T^GTACA 2786 TGTACA DraIII 607 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 1269 CACCCTGTG DsaI 332 CCATGG C^C[GA][CT]GG 838 CCACGG EaeI 256 TGGCCG [CT]^GGCC[GA] 1193 TGGCCA EcoRI 745 GAATTC G^AATTC 2116 GAATTC EcoRV 1879 GATATC GAT^ATC 2239 GATATC FbaI 2689 TGATCA T^GATCA 3482 TGATCA HaeII 237 GGCGCC [GA]GCGC^[CT] 661 GGCGCT HgaI 1865 GACGC GACGC(5/10) 1806 GCGTC MspA1I 696 CAGCGG C[AC]G^C[GT]G 855 CCGCTG NciI 183 CCCGG CC^[GC]GG 184 CCGGG NspI 1868 GCATGC [GA]CATG^[CT] 2186 ACATGC PflMI 1185 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1268 CCACCCTGTGG SspBI 1515 TGTACA T^GTACA 2786 TGTACA Van91I 1185 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1268 CCACCCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BanI BanII BbvI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NsiI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI