##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BsaAI BsaBI BseAI
BsgI BsiEI BsiWI BsmI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
BstBI BstEII BstPI Bsu36I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I FseI FspI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 211 CGCG CG^CG
AcyI 237 GGCGCC G[GA]^CG[CT]C
AflIII 2888 ACACGT A^C[GA][CT]GT
AlwNI 3044 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 183 CCCGGG C^[CT]CG[GA]G
BalI 1193 TGGCCA TGG^CCA
BamHI 1997 GGATCC G^GATCC
BbeI 237 GGCGCC GGCGC^C
BbsI 2719 GTCTTC GAAGAC(2/6)
BpuAI 2719 GTCTTC GAAGAC(2/6)
BsaHI 237 GGCGCC G[GA]^CG[CT]C
BsaWI 753 ACCGGA [AT]^CCGG[AT]
BsmBI 1807 CGTCTC CGTCTC(1/5)
BsoBI 183 CCCGGG C^[CT]CG[GA]G
BspDI 1972 ATCGAT AT^CGAT
BsrFI 258 GCCGGT [GA]^CCGG[CT]
Bst1107I 3127 GTATAC GTA^TAC
BstAPI 3043 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 211 CGCG CG^CG
BstZ17I 3127 GTATAC GTA^TAC
Cfr10I 258 GCCGGT [GA]^CCGG[CT]
ClaI 1972 ATCGAT AT^CGAT
DraI 2079 TTTAAA TTT^AAA
DrdI 2043 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 486 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 237 GGCGCC G[GA]^CG[CT]C
HpaI 514 GTTAAC GTT^AAC
KasI 237 GGCGCC G^GCGCC
MaeII 2890 ACGT A^CGT
MluNI 1193 TGGCCA TGG^CCA
MscI 1193 TGGCCA TGG^CCA
MvnI 211 CGCG CG^CG
NarI 237 GGCGCC GG^CGCC
NcoI 332 CCATGG C^CATGG
SapI 1162 GAAGAGC GCTCTTC(1/4)
ScaI 109 AGTACT AGT^ACT
SfoI 237 GGCGCC GGC^GCC
SmaI 183 CCCGGG CCC^GGG
SphI 1868 GCATGC GCATG^C
StuI 434 AGGCCT AGG^CCT
TfiI 2638 GAATC G^A[AT]TC
XmaCI 183 CCCGGG C^CCGGG
XmaI 183 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BclI 2689 TGATCA T^GATCA
3482 TGATCA
BcnI 183 CCCGG CC^[GC]GG
184 CCGGG
BsaI 377 GAGACC GGTCTC(1/5)
2387 GAGACC
Bsp1407I 1515 TGTACA T^GTACA
2786 TGTACA
BsrGI 1515 TGTACA T^GTACA
2786 TGTACA
DraIII 607 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1269 CACCCTGTG
DsaI 332 CCATGG C^C[GA][CT]GG
838 CCACGG
EaeI 256 TGGCCG [CT]^GGCC[GA]
1193 TGGCCA
EcoRI 745 GAATTC G^AATTC
2116 GAATTC
EcoRV 1879 GATATC GAT^ATC
2239 GATATC
FbaI 2689 TGATCA T^GATCA
3482 TGATCA
HaeII 237 GGCGCC [GA]GCGC^[CT]
661 GGCGCT
HgaI 1865 GACGC GACGC(5/10)
1806 GCGTC
MspA1I 696 CAGCGG C[AC]G^C[GT]G
855 CCGCTG
NciI 183 CCCGG CC^[GC]GG
184 CCGGG
NspI 1868 GCATGC [GA]CATG^[CT]
2186 ACATGC
PflMI 1185 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1268 CCACCCTGTGG
SspBI 1515 TGTACA T^GTACA
2786 TGTACA
Van91I 1185 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1268 CCACCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BanII BbvI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI