##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BbvCI BcgI BciVI BfrI
BlnI BsaBI BspHI BspLU11I BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
DraI DraIII Eam1105I Eco47III Eco81I
EcoRI EcoRV EcoT22I FseI HpaI
KpnI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I RcaI SalI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1657 GACGTC GACGT^C
AflIII 917 ACGTGT A^C[GA][CT]GT
AspI 1993 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1440 GGATCC G^GATCC
BbrPI 1125 CACGTG CAC^GTG
BclI 1187 TGATCA T^GATCA
BglI 2409 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2568 AGATCT A^GATCT
BsaI 2189 GGTCTC GGTCTC(1/5)
BsiWI 500 CGTACG C^GTACG
BsmBI 2453 GAGACG CGTCTC(1/5)
Bsp1407I 560 TGTACA T^GTACA
BspDI 565 ATCGAT AT^CGAT
BsrDI 704 GCAATG GCAATG(2/0)
BsrGI 560 TGTACA T^GTACA
BssSI 1964 CACGAG CACGAG(-5/-1)
BstEII 1825 GGTGACC G^GT[ACGT]ACC
BstPI 1825 GGTGACC G^GT[ACGT]ACC
ClaI 565 ATCGAT AT^CGAT
CpoI 22 CGGTCCG CG^G[AT]CCG
EagI 1117 CGGCCG C^GGCCG
EclXI 1117 CGGCCG C^GGCCG
Eco52I 1117 CGGCCG C^GGCCG
EcoNI 1631 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1825 GGTGACC G^GT[ACGT]ACC
FbaI 1187 TGATCA T^GATCA
HindIII 715 AAGCTT A^AGCTT
PaeR7I 475 CTCGAG C^TCGAG
PflFI 1993 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1125 CACGTG CAC^GTG
PmlI 1125 CACGTG CAC^GTG
PvuI 1908 CGATCG CGAT^CG
RsrII 22 CGGTCCG CG^G[AT]CCG
ScaI 2126 AGTACT AGT^ACT
SexAI 1301 ACCAGGT A^CC[AT]GGT
SspBI 560 TGTACA T^GTACA
Tsp509I 3098 AATT ^AATT
Tth111I 1993 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 475 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1012 GTCTAC GT^[AC][GT]AC
2542 GTCTAC
AccIII 25 TCCGGA T^CCGGA
2241 TCCGGA
Alw44I 411 GTGCAC G^TGCAC
1892 GTGCAC
ApaI 1048 GGGCCC GGGCC^C
1069 GGGCCC
ApaLI 411 GTGCAC G^TGCAC
1892 GTGCAC
BalI 1602 TGGCCA TGG^CCA
2885 TGGCCA
BbsI 1039 GTCTTC GAAGAC(2/6)
1432 GTCTTC
BfaI 2285 CTAG C^TAG
3211 CTAG
BlpI 676 GCTGAGC GC^T[ACGT]AGC
1902 GCTCAGC
Bpu1102I 676 GCTGAGC GC^T[ACGT]AGC
1902 GCTCAGC
BpuAI 1039 GTCTTC GAAGAC(2/6)
1432 GTCTTC
BsaWI 25 TCCGGA [AT]^CCGG[AT]
2241 TCCGGA
BseAI 25 TCCGGA T^CCGGA
2241 TCCGGA
BsmI 295 GAATGC GAATGC(1/-1)
1716 GAATGC
BspEI 25 TCCGGA T^CCGGA
2241 TCCGGA
BspMI 230 ACCTGC ACCTGC(4/8)
2546 ACCTGC
BsrBI 1779 CCGCTC CCGCTC(-3/-3)
736 GAGCGG
BstXI 1539 CCAGGCGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2487 CCAGCCAGTTGG
CelII 676 GCTGAGC GC^T[ACGT]AGC
1902 GCTCAGC
DrdI 916 GACGTGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2513 GACCACACAGTC
HincII 319 GTCAAC GT[CT]^[GA]AC
826 GTCAAC
HindII 319 GTCAAC GT[CT]^[GA]AC
826 GTCAAC
HphI 1185 GGTGA GGTGA(8/7)
1825 GGTGA
MaeI 2285 CTAG C^TAG
3211 CTAG
MluNI 1602 TGGCCA TGG^CCA
2885 TGGCCA
MroI 25 TCCGGA T^CCGGA
2241 TCCGGA
MscI 1602 TGGCCA TGG^CCA
2885 TGGCCA
NcoI 1019 CCATGG C^CATGG
2273 CCATGG
NspI 191 GCATGT [GA]CATG^[CT]
1977 GCATGT
PpuMI 49 AGGACCT [GA]G^G[AT]CC[CT]
1580 GGGACCC
PshAI 473 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
805 GACGGTGGTC
PstI 1237 CTGCAG CTGCA^G
1897 CTGCAG
StuI 1607 AGGCCT AGG^CCT
3028 AGGCCT
TfiI 161 GAATC G^A[AT]TC
1842 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
AviII BanI BanII BbeI BbvI
BcnI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI