##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI ApoI AscI AseI
AsnI Asp718I AspI AvrII BbrPI
BcgI BciVI BclI BfrI BglI
BlnI BsaAI BsaBI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MunI
NdeI NheI NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SexAI SfiI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 901 GTCTAC GT^[AC][GT]AC
AhdI 1831 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 265 GTGCAC G^TGCAC
AocI 12 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1865 AGCGCT AGC^GCT
ApaLI 265 GTGCAC G^TGCAC
Asp700I 1095 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1831 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 3106 GTCTTC GAAGAC(2/6)
BbvCI 1875 CCTCAGC CCTCAGC(-5/-2)
BglII 603 AGATCT A^GATCT
BpuAI 3106 GTCTTC GAAGAC(2/6)
BsmBI 2181 CGTCTC CGTCTC(1/5)
BssSI 2203 CACGAG CACGAG(-5/-1)
Bsu36I 12 CCTGAGG CC^T[ACGT]AGG
Eam1105I 1831 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1865 AGCGCT AGC^GCT
Eco81I 12 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1744 ATGCAT ATGCA^T
NaeI 928 GCCGGC GCC^GGC
NcoI 896 CCATGG C^CATGG
NgoMIV 928 GCCGGC G^CCGGC
NsiI 1744 ATGCAT ATGCA^T
PaeR7I 1147 CTCGAG C^TCGAG
PflMI 2061 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 995 AGTACT AGT^ACT
Van91I 2061 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 600 TCTAGA T^CTAGA
XcmI 2891 CCACCCTGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1147 CTCGAG C^TCGAG
XmnI 1095 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AviII 2115 TGCGCA TGC^GCA
2453 TGCGCA
BalI 2168 TGGCCA TGG^CCA
2415 TGGCCA
BbeI 8 GGCGCC GGCGC^C
1160 GGCGCC
BsaI 173 GAGACC GGTCTC(1/5)
2999 GGTCTC
BsrFI 928 GCCGGC [GA]^CCGG[CT]
1541 ACCGGC
BstAPI 1512 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2097 GCACTACCTGC
BstBI 791 TTCGAA TT^CGAA
860 TTCGAA
Cfr10I 928 GCCGGC [GA]^CCGG[CT]
1541 ACCGGC
EcoNI 2073 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2166 CCTGGCCAAGG
EcoT14I 896 CCATGG C^C[AT][AT]GG
2171 CCAAGG
FspI 2115 TGCGCA TGC^GCA
2453 TGCGCA
KasI 8 GGCGCC G^GCGCC
1160 GGCGCC
MluNI 2168 TGGCCA TGG^CCA
2415 TGGCCA
MscI 2168 TGGCCA TGG^CCA
2415 TGGCCA
MslI 39 CACCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1548 CATCCAGATG
NarI 8 GGCGCC GG^CGCC
1160 GGCGCC
NspV 791 TTCGAA TT^CGAA
860 TTCGAA
SacI 463 GAGCTC GAGCT^C
1927 GAGCTC
SapI 1924 GAAGAGC GCTCTTC(1/4)
351 GCTCTTC
SbfI 1683 CCTGCAGG CCTGCA^GG
2844 CCTGCAGG
SfoI 8 GGCGCC GGC^GCC
1160 GGCGCC
SfuI 791 TTCGAA TT^CGAA
860 TTCGAA
SmaI 2571 CCCGGG CCC^GGG
3027 CCCGGG
SmlI 1071 CTCAAG C^T[CT][GA]AG
1147 CTCGAG
Sse8387I 1683 CCTGCAGG CCTGCA^GG
2844 CCTGCAGG
StyI 896 CCATGG C^C[AT][AT]GG
2171 CCAAGG
Tsp509I 1008 AATT ^AATT
1981 AATT
XmaCI 2571 CCCGGG C^CCGGG
3027 CCCGGG
XmaI 2571 CCCGGG C^CCGGG
3027 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BamHI BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII