##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BspDI
BspEI BspHI BsrFI BssHII Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
HaeII HincII HindII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 1977 GTATAC GT^[AC][GT]AC
AclI 1797 AACGTT AA^CGTT
AcyI 222 GACGCC G[GA]^CG[CT]C
AflIII 1888 ACATGT A^C[GA][CT]GT
Alw44I 1863 GTGCAC G^TGCAC
ApaLI 1863 GTGCAC G^TGCAC
AspI 621 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 136 GGACC G^G[AT]CC
AviII 142 TGCGCA TGC^GCA
BalI 1831 TGGCCA TGG^CCA
BbsI 1915 GTCTTC GAAGAC(2/6)
BlpI 173 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 173 GCTCAGC GC^T[ACGT]AGC
BpuAI 1915 GTCTTC GAAGAC(2/6)
BsaHI 222 GACGCC G[GA]^CG[CT]C
BsaI 1092 GGTCTC GGTCTC(1/5)
BsmBI 26 CGTCTC CGTCTC(1/5)
Bsp1407I 1057 TGTACA T^GTACA
BspLU11I 1888 ACATGT A^CATGT
BspMI 987 GCAGGT ACCTGC(4/8)
BsrBI 242 CCGCTC CCGCTC(-3/-3)
BsrDI 961 GCAATG GCAATG(2/0)
BsrGI 1057 TGTACA T^GTACA
BssSI 1410 CTCGTG CACGAG(-5/-1)
Bst1107I 1977 GTATAC GTA^TAC
BstBI 2025 TTCGAA TT^CGAA
BstEII 2158 GGTTACC G^GT[ACGT]ACC
BstPI 2158 GGTTACC G^GT[ACGT]ACC
BstXI 1834 CCATCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1977 GTATAC GTA^TAC
CelII 173 GCTCAGC GC^T[ACGT]AGC
EcoNI 1589 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2158 GGTTACC G^GT[ACGT]ACC
EcoT14I 700 CCTTGG C^C[AT][AT]GG
FspI 142 TGCGCA TGC^GCA
HgaI 222 GACGC GACGC(5/10)
Hin1I 222 GACGCC G[GA]^CG[CT]C
MluNI 1831 TGGCCA TGG^CCA
MscI 1831 TGGCCA TGG^CCA
NspV 2025 TTCGAA TT^CGAA
PflFI 621 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 898 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1797 AACGTT AA^CGTT
PvuII 399 CAGCTG CAG^CTG
SacI 423 GAGCTC GAGCT^C
ScaI 1009 AGTACT AGT^ACT
SfuI 2025 TTCGAA TT^CGAA
SmaI 188 CCCGGG CCC^GGG
SspBI 1057 TGTACA T^GTACA
StyI 700 CCTTGG C^C[AT][AT]GG
Tth111I 621 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 898 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 188 CCCGGG C^CCGGG
XmaI 188 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 100 CGCG CG^CG
149 CGCG
AcsI 567 GAATTT [GA]^AATT[CT]
755 GAATTT
AlwI 303 GGATC GGATC(4/5)
808 GGATC
AocI 37 CCTCAGG CC^T[ACGT]AGG
327 CCTAAGG
ApaI 332 GGGCCC GGGCC^C
1487 GGGCCC
ApoI 567 GAATTT [GA]^AATT[CT]
755 GAATTT
AspHI 423 GAGCTC G[AT]GC[AT]^C
1863 GTGCAC
AvaI 188 CCCGGG C^[CT]CG[GA]G
1791 CTCGGG
BanI 281 GGTGCC G^G[CT][GA]CC
1878 GGTGCC
BclI 844 TGATCA T^GATCA
857 TGATCA
BglII 1200 AGATCT A^GATCT
1220 AGATCT
BpmI 1986 CTCCAG CTGGAG(16/14)
1013 CTGGAG
BsiHKAI 423 GAGCTC G[AT]GC[AT]^C
1863 GTGCAC
BsoBI 188 CCCGGG C^[CT]CG[GA]G
1791 CTCGGG
BstAPI 145 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1865 GCACTGACTGC
BstUI 100 CGCG CG^CG
149 CGCG
Bsu36I 37 CCTCAGG CC^T[ACGT]AGG
327 CCTAAGG
DraIII 146 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1906 CACCCTGTG
Eco81I 37 CCTCAGG CC^T[ACGT]AGG
327 CCTAAGG
FbaI 844 TGATCA T^GATCA
857 TGATCA
MvnI 100 CGCG CG^CG
149 CGCG
PstI 261 CTGCAG CTGCA^G
1355 CTGCAG
SfaNI 167 GATGC GCATC(5/9)
1728 GATGC
StuI 466 AGGCCT AGG^CCT
683 AGGCCT
TaqI 1922 TCGA T^CGA
2026 TCGA
TthHB8I 1922 TCGA T^CGA
2026 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwNI Asp700I
BanII BbvI BcnI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI