##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I AspEI AvrII BalI
BbeI BbrPI BcgI BglI BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI HaeII HgaI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1810 GGTACC G^GTACC
AccI 33 GTAGAC GT^[AC][GT]AC
AclI 2402 AACGTT AA^CGTT
AflII 2912 CTTAAG C^TTAAG
AflIII 29 ACATGT A^C[GA][CT]GT
AocI 1116 CCTAAGG CC^T[ACGT]AGG
Asp718I 1810 GGTACC G^GTACC
AspI 1050 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2061 CTCGGG C^[CT]CG[GA]G
AviII 2942 TGCGCA TGC^GCA
BclI 2211 TGATCA T^GATCA
BcnI 2027 CCGGG CC^[GC]GG
BfrI 2912 CTTAAG C^TTAAG
BglII 1008 AGATCT A^GATCT
BsaAI 2924 CACGTA [CT]AC^GT[GA]
BsaBI 698 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2645 GGTCTC GGTCTC(1/5)
BsmBI 963 CGTCTC CGTCTC(1/5)
BsoBI 2061 CTCGGG C^[CT]CG[GA]G
BspLU11I 29 ACATGT A^CATGT
Bsu36I 1116 CCTAAGG CC^T[ACGT]AGG
DrdI 1339 GACATCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1116 CCTAAGG CC^T[ACGT]AGG
EcoT22I 1237 ATGCAT ATGCA^T
FbaI 2211 TGATCA T^GATCA
FspI 2942 TGCGCA TGC^GCA
HincII 1897 GTCAAC GT[CT]^[GA]AC
HindII 1897 GTCAAC GT[CT]^[GA]AC
KpnI 1810 GGTACC GGTAC^C
MamI 698 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 2027 CCGGG CC^[GC]GG
NsiI 1237 ATGCAT ATGCA^T
PflFI 1050 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1722 CCACCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2402 AACGTT AA^CGTT
SapI 1207 GCTCTTC GCTCTTC(1/4)
SmlI 2912 CTTAAG C^T[CT][GA]AG
SpeI 1855 ACTAGT A^CTAGT
SphI 235 GCATGC GCATG^C
StuI 2054 AGGCCT AGG^CCT
Tth111I 1050 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1722 CCACCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 669 GTGCAC G^TGCAC
1326 GTGCAC
ApaLI 669 GTGCAC G^TGCAC
1326 GTGCAC
BamHI 531 GGATCC G^GATCC
2636 GGATCC
BbvCI 737 CCTCAGC CCTCAGC(-5/-2)
1842 CCTCAGC
BciVI 90 GGATAC GTATCC(6/5)
2066 GTATCC
BlpI 517 GCTTAGC GC^T[ACGT]AGC
731 GCTCAGC
Bpu1102I 517 GCTTAGC GC^T[ACGT]AGC
731 GCTCAGC
BseRI 1689 CTCCTC GAGGAG(10/8)
318 GAGGAG
BspHI 222 TCATGA T^CATGA
3005 TCATGA
BstBI 1081 TTCGAA TT^CGAA
2406 TTCGAA
BstXI 1045 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2199 CCAGGAGTTTGG
CelII 517 GCTTAGC GC^T[ACGT]AGC
731 GCTCAGC
DraIII 462 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1802 CACCAAGTG
EcoNI 2046 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2193 CCTGCCCCAGG
EcoRI 136 GAATTC G^AATTC
951 GAATTC
HapII 1383 CCGG C^CGG
2027 CCGG
HpaII 1383 CCGG C^CGG
2027 CCGG
MslI 230 CATAAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1604 CATCTGTGTG
MspI 1383 CCGG C^CGG
2027 CCGG
NspV 1081 TTCGAA TT^CGAA
2406 TTCGAA
PleI 226 GAGTC GAGTC(4/5)
1346 GAGTC
RcaI 222 TCATGA T^CATGA
3005 TCATGA
SexAI 510 ACCTGGT A^CC[AT]GGT
1051 ACCTGGT
SfuI 1081 TTCGAA TT^CGAA
2406 TTCGAA
TaqI 1082 TCGA T^CGA
2407 TCGA
TthHB8I 1082 TCGA T^CGA
2407 TCGA
XcmI 2499 CCACACAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2999 CCAGGGTCATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII