##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccI AccIII AcyI AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspI AvrII BalI BamHI
BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BsrBI Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FbaI FseI HaeII HgaI
Hin1I HindIII KasI KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmeI PshAI PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3004 GGTACC G^GTACC
AccII 57 CGCG CG^CG
AclI 721 AACGTT AA^CGTT
AflII 2197 CTTAAG C^TTAAG
AseI 2642 ATTAAT AT^TAAT
AsnI 2642 ATTAAT AT^TAAT
Asp718I 3004 GGTACC G^GTACC
AspHI 985 GTGCTC G[AT]GC[AT]^C
AvaI 1772 CTCGAG C^[CT]CG[GA]G
BbrPI 2736 CACGTG CAC^GTG
BcnI 1746 CCGGG CC^[GC]GG
BfrI 2197 CTTAAG C^TTAAG
BsaI 1437 GGTCTC GGTCTC(1/5)
BseRI 1952 GAGGAG GAGGAG(10/8)
BsgI 2050 CTGCAC GTGCAG(16/14)
BsiHKAI 985 GTGCTC G[AT]GC[AT]^C
BsmBI 1527 CGTCTC CGTCTC(1/5)
BsoBI 1772 CTCGAG C^[CT]CG[GA]G
BsrDI 2768 CATTGC GCAATG(2/0)
BsrFI 512 ACCGGC [GA]^CCGG[CT]
BssHII 56 GCGCGC G^CGCGC
BssSI 1601 CTCGTG CACGAG(-5/-1)
BstAPI 1351 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 969 TTCGAA TT^CGAA
BstUI 57 CGCG CG^CG
BstYI 16 GGATCT [GA]^GATC[CT]
Cfr10I 512 ACCGGC [GA]^CCGG[CT]
EcoRI 3025 GAATTC G^AATTC
EcoRV 1767 GATATC GAT^ATC
FauI 2184 GCGGG CCCGC(4/6)
HpaI 286 GTTAAC GTT^AAC
KpnI 3004 GGTACC GGTAC^C
MflI 16 GGATCT [GA]^GATC[CT]
MvnI 57 CGCG CG^CG
NciI 1746 CCGGG CC^[GC]GG
NspV 969 TTCGAA TT^CGAA
PaeR7I 1772 CTCGAG C^TCGAG
PflMI 1708 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2736 CACGTG CAC^GTG
PmlI 2736 CACGTG CAC^GTG
PshBI 2642 ATTAAT AT^TAAT
Psp1406I 721 AACGTT AA^CGTT
SfuI 969 TTCGAA TT^CGAA
Van91I 1708 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 113 TCTAGA T^CTAGA
XhoI 1772 CTCGAG C^TCGAG
XhoII 16 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AhdI 440 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
598 GACTCCAGGTC
AlwNI 1352 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1877 CAGACCCTG
Asp700I 465 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2550 GAAAAATTTC
AspEI 440 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
598 GACTCCAGGTC
AviII 2139 TGCGCA TGC^GCA
2161 TGCGCA
BbsI 1565 GAAGAC GAAGAC(2/6)
1591 GAAGAC
BmyI 985 GTGCTC G[AGT]GC[ACT]^C
1869 GTGCCC
BpuAI 1565 GAAGAC GAAGAC(2/6)
1591 GAAGAC
BsaAI 2736 CACGTG [CT]AC^GT[GA]
3015 TACGTG
BsaBI 1287 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1455 GATTGACATC
Bsp1286I 985 GTGCTC G[AGT]GC[ACT]^C
1869 GTGCCC
BspLU11I 3036 ACATGT A^CATGT
3120 ACATGT
BspMI 1231 ACCTGC ACCTGC(4/8)
1683 ACCTGC
DraII 708 AGGTCCT [GA]G^G[ACGT]CC[CT]
1885 GGGACCT
DsaI 773 CCATGG C^C[GA][CT]GG
1025 CCATGG
Eam1105I 440 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
598 GACTCCAGGTC
EcoO109I 708 AGGTCCT [GA]G^G[ACGT]CC[CT]
1885 GGGACCT
EcoT14I 773 CCATGG C^C[AT][AT]GG
1025 CCATGG
FspI 2139 TGCGCA TGC^GCA
2161 TGCGCA
HapII 513 CCGG C^CGG
1746 CCGG
HincII 286 GTTAAC GT[CT]^[GA]AC
2885 GTCAAC
HindII 286 GTTAAC GT[CT]^[GA]AC
2885 GTCAAC
HpaII 513 CCGG C^CGG
1746 CCGG
MamI 1287 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1455 GATTGACATC
MspI 513 CCGG C^CGG
1746 CCGG
NcoI 773 CCATGG C^CATGG
1025 CCATGG
NspI 3036 ACATGT [GA]CATG^[CT]
3120 ACATGT
PpuMI 708 AGGTCCT [GA]G^G[AT]CC[CT]
1885 GGGACCT
SpeI 2762 ACTAGT A^CTAGT
2873 ACTAGT
StyI 773 CCATGG C^C[AT][AT]GG
1025 CCATGG
XcmI 1361 CCAGGGTCGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1650 CCAAGTGAAAGATGG
XmnI 465 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2550 GAAAAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BanI BbvI
BfaI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I