##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AviII AvrII BbeI BbvCI BcgI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BseAI BsiWI
BsmI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Alw44I 3021 GTGCAC G^TGCAC
ApaLI 3021 GTGCAC G^TGCAC
AseI 2788 ATTAAT AT^TAAT
AsnI 2788 ATTAAT AT^TAAT
BamHI 2324 GGATCC G^GATCC
BanI 1972 GGTGCC G^G[CT][GA]CC
BanII 1789 GAGCCC G[GA]GC[CT]^C
BbrPI 2522 CACGTG CAC^GTG
BciVI 1932 GTATCC GTATCC(6/5)
BglII 3209 AGATCT A^GATCT
BsaAI 2522 CACGTG [CT]AC^GT[GA]
BsaWI 3001 ACCGGA [AT]^CCGG[AT]
BseRI 2115 CTCCTC GAGGAG(10/8)
BsiEI 17 CGACCG CG[GA][CT]^CG
BsmBI 3 CGTCTC CGTCTC(1/5)
BspHI 2885 TCATGA T^CATGA
BspMI 2660 ACCTGC ACCTGC(4/8)
BssSI 2504 CACGAG CACGAG(-5/-1)
BstEII 2140 GGTGACC G^GT[ACGT]ACC
BstPI 2140 GGTGACC G^GT[ACGT]ACC
BstXI 2238 CCATATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1635 TTTAAA TTT^AAA
DsaI 1976 CCATGG C^C[GA][CT]GG
EcoO65I 2140 GGTGACC G^GT[ACGT]ACC
EcoRI 2891 GAATTC G^AATTC
HindIII 1871 AAGCTT A^AGCTT
MslI 2059 CATAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1976 CCATGG C^CATGG
PmaCI 2522 CACGTG CAC^GTG
PmlI 2522 CACGTG CAC^GTG
PshBI 2788 ATTAAT AT^TAAT
RcaI 2885 TCATGA T^CATGA
SapI 107 GAAGAGC GCTCTTC(1/4)
SspI 3147 AATATT AAT^ATT
StuI 698 AGGCCT AGG^CCT
TaqI 2656 TCGA T^CGA
TthHB8I 2656 TCGA T^CGA
XbaI 1059 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspHI 1892 GTGCTC G[AT]GC[AT]^C
3021 GTGCAC
AvaI 209 CCCGAG C^[CT]CG[GA]G
2684 CTCGGG
AvaII 418 GGACC G^G[AT]CC
849 GGACC
BalI 2381 TGGCCA TGG^CCA
3173 TGGCCA
BbsI 2054 GTCTTC GAAGAC(2/6)
2335 GTCTTC
BcnI 125 CCCGG CC^[GC]GG
1377 CCGGG
BpuAI 2054 GTCTTC GAAGAC(2/6)
2335 GTCTTC
BsiHKAI 1892 GTGCTC G[AT]GC[AT]^C
3021 GTGCAC
BsmFI 2156 GGGAC GGGAC(10/14)
41 GTCCC
BsoBI 209 CCCGAG C^[CT]CG[GA]G
2684 CTCGGG
BspLU11I 1928 ACATGT A^CATGT
2220 ACATGT
CfoI 151 GCGC GCG^C
2408 GCGC
EaeI 2381 TGGCCA [CT]^GGCC[GA]
3173 TGGCCA
EcoNI 883 CCTAACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2364 CCTCTCTGAGG
EcoRV 1528 GATATC GAT^ATC
2455 GATATC
HaeII 150 GGCGCT [GA]GCGC^[CT]
2407 GGCGCT
HhaI 151 GCGC GCG^C
2408 GCGC
HinP1I 151 GCGC G^CGC
2408 GCGC
MaeII 2211 ACGT A^CGT
2523 ACGT
MfeI 758 CAATTG C^AATTG
2831 CAATTG
MluNI 2381 TGGCCA TGG^CCA
3173 TGGCCA
MscI 2381 TGGCCA TGG^CCA
3173 TGGCCA
MunI 758 CAATTG C^AATTG
2831 CAATTG
NciI 125 CCCGG CC^[GC]GG
1377 CCGGG
SfaNI 2308 GATGC GCATC(5/9)
3052 GATGC
SphI 983 GCATGC GCATG^C
2552 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AspI BbvI BfaI BglI BmyI
BpmI BsaJI BsgI BsiYI BslI
BsmAI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII EarI Eco81I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflFI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfcI SmlI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I XcmI
XhoII XmnI