##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AvaII AviII BalI
BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII
BpmI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I KasI KpnI KspI MluI
MluNI MroI MscI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
StuI Tth111I Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Alw44I 1419 GTGCAC G^TGCAC
AlwI 1391 GGATC GGATC(4/5)
ApaLI 1419 GTGCAC G^TGCAC
AseI 860 ATTAAT AT^TAAT
AsnI 860 ATTAAT AT^TAAT
AvaI 1751 CCCGGG C^[CT]CG[GA]G
AvrII 2560 CCTAGG C^CTAGG
BbrPI 2984 CACGTG CAC^GTG
BlnI 2560 CCTAGG C^CTAGG
BsaAI 2984 CACGTG [CT]AC^GT[GA]
BsaBI 1034 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 2835 GGGAC GGGAC(10/14)
BsoBI 1751 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3051 TGTACA T^GTACA
BsrGI 3051 TGTACA T^GTACA
Bst1107I 2664 GTATAC GTA^TAC
BstNI 94 CCAGG CC^[AT]GG
BstYI 1391 GGATCT [GA]^GATC[CT]
BstZ17I 2664 GTATAC GTA^TAC
EcoRII 94 CCAGG ^CC[AT]GG
EcoRV 257 GATATC GAT^ATC
HgaI 2011 GCGTC GACGC(5/10)
HindIII 2290 AAGCTT A^AGCTT
MaeII 2985 ACGT A^CGT
MamI 1034 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1884 CAATTG C^AATTG
MflI 1391 GGATCT [GA]^GATC[CT]
MspA1I 1675 CAGCTG C[AC]G^C[GT]G
MunI 1884 CAATTG C^AATTG
MvaI 94 CCAGG CC^[AT]GG
PmaCI 2984 CACGTG CAC^GTG
PmlI 2984 CACGTG CAC^GTG
PshBI 860 ATTAAT AT^TAAT
PstI 1309 CTGCAG CTGCA^G
PvuII 1675 CAGCTG CAG^CTG
SmaI 1751 CCCGGG CCC^GGG
SpeI 896 ACTAGT A^CTAGT
SspBI 3051 TGTACA T^GTACA
SwaI 1069 ATTTAAAT ATTT^AAAT
XbaI 135 TCTAGA T^CTAGA
XhoII 1391 GGATCT [GA]^GATC[CT]
XmaCI 1751 CCCGGG C^CCGGG
XmaI 1751 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 2664 GTATAC GT^[AC][GT]AC
2903 GTAGAC
AflII 172 CTTAAG C^TTAAG
1320 CTTAAG
AspHI 1419 GTGCAC G[AT]GC[AT]^C
2740 GTGCTC
BbsI 2891 GAAGAC GAAGAC(2/6)
2989 GTCTTC
BfrI 172 CTTAAG C^TTAAG
1320 CTTAAG
BlpI 1605 GCTTAGC GC^T[ACGT]AGC
2742 GCTCAGC
BmyI 1419 GTGCAC G[AGT]GC[ACT]^C
2740 GTGCTC
Bpu1102I 1605 GCTTAGC GC^T[ACGT]AGC
2742 GCTCAGC
BpuAI 2891 GAAGAC GAAGAC(2/6)
2989 GTCTTC
BsaI 1304 GAGACC GGTCTC(1/5)
487 GGTCTC
BsgI 1907 CTGCAC GTGCAG(16/14)
2129 GTGCAG
BsiHKAI 1419 GTGCAC G[AT]GC[AT]^C
2740 GTGCTC
BsmI 1350 GCATTC GAATGC(1/-1)
2254 GCATTC
Bsp1286I 1419 GTGCAC G[AGT]GC[ACT]^C
2740 GTGCTC
BspLU11I 2175 ACATGT A^CATGT
2277 ACATGT
BspMI 1307 ACCTGC ACCTGC(4/8)
1905 ACCTGC
BstAPI 1825 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2131 GCAGCCCATGC
BstXI 1689 CCAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2594 CCAAGTGTATGG
CelII 1605 GCTTAGC GC^T[ACGT]AGC
2742 GCTCAGC
Cfr13I 1889 GGGCC G^G[ACGT]CC
2582 GGGCC
DsaI 1742 CCATGG C^C[GA][CT]GG
2676 CCATGG
EarI 2486 CTCTTC CTCTTC(1/4)
76 GAAGAG
EcoNI 145 CCTTAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2748 CCTCCCCAAGG
HpaI 1733 GTTAAC GTT^AAC
2019 GTTAAC
Ksp632I 2486 CTCTTC CTCTTC(1/4)
76 GAAGAG
NcoI 1742 CCATGG C^CATGG
2676 CCATGG
NdeI 603 CATATG CA^TATG
1843 CATATG
NspI 2175 ACATGT [GA]CATG^[CT]
2277 ACATGT
PshAI 2568 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2982 GACACGTGTC
Sau96I 1889 GGGCC G^G[ACGT]CC
2582 GGGCC
SfaNI 1747 GATGC GCATC(5/9)
1822 GATGC
SmlI 172 CTTAAG C^T[CT][GA]AG
1320 CTTAAG
SspI 1126 AATATT AAT^ATT
2922 AATATT
TaqI 2241 TCGA T^CGA
2871 TCGA
TthHB8I 2241 TCGA T^CGA
2871 TCGA
XcmI 214 CCATGTGTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2792 CCAAGCCGGATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI BbvI BcnI BfaI BsaJI
BseRI BsiYI BslI BsmAI BspHI
BsrI BssKI BstF5I DdeI DpnII
DraI EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspI
MwoI NciI NdeII NlaIII NlaIV
PleI RcaI RsaI Sau3AI ScaI
ScrFI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI