################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaII AviII BalI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BpmI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstPI BstUI Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I StuI Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Alw44I 1419 GTGCAC G^TGCAC AlwI 1391 GGATC GGATC(4/5) ApaLI 1419 GTGCAC G^TGCAC AseI 860 ATTAAT AT^TAAT AsnI 860 ATTAAT AT^TAAT AvaI 1751 CCCGGG C^[CT]CG[GA]G AvrII 2560 CCTAGG C^CTAGG BbrPI 2984 CACGTG CAC^GTG BlnI 2560 CCTAGG C^CTAGG BsaAI 2984 CACGTG [CT]AC^GT[GA] BsaBI 1034 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmFI 2835 GGGAC GGGAC(10/14) BsoBI 1751 CCCGGG C^[CT]CG[GA]G Bsp1407I 3051 TGTACA T^GTACA BsrGI 3051 TGTACA T^GTACA Bst1107I 2664 GTATAC GTA^TAC BstNI 94 CCAGG CC^[AT]GG BstYI 1391 GGATCT [GA]^GATC[CT] BstZ17I 2664 GTATAC GTA^TAC EcoRII 94 CCAGG ^CC[AT]GG EcoRV 257 GATATC GAT^ATC HgaI 2011 GCGTC GACGC(5/10) HindIII 2290 AAGCTT A^AGCTT MaeII 2985 ACGT A^CGT MamI 1034 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1884 CAATTG C^AATTG MflI 1391 GGATCT [GA]^GATC[CT] MspA1I 1675 CAGCTG C[AC]G^C[GT]G MunI 1884 CAATTG C^AATTG MvaI 94 CCAGG CC^[AT]GG PmaCI 2984 CACGTG CAC^GTG PmlI 2984 CACGTG CAC^GTG PshBI 860 ATTAAT AT^TAAT PstI 1309 CTGCAG CTGCA^G PvuII 1675 CAGCTG CAG^CTG SmaI 1751 CCCGGG CCC^GGG SpeI 896 ACTAGT A^CTAGT SspBI 3051 TGTACA T^GTACA SwaI 1069 ATTTAAAT ATTT^AAAT XbaI 135 TCTAGA T^CTAGA XhoII 1391 GGATCT [GA]^GATC[CT] XmaCI 1751 CCCGGG C^CCGGG XmaI 1751 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccI 2664 GTATAC GT^[AC][GT]AC 2903 GTAGAC AflII 172 CTTAAG C^TTAAG 1320 CTTAAG AspHI 1419 GTGCAC G[AT]GC[AT]^C 2740 GTGCTC BbsI 2891 GAAGAC GAAGAC(2/6) 2989 GTCTTC BfrI 172 CTTAAG C^TTAAG 1320 CTTAAG BlpI 1605 GCTTAGC GC^T[ACGT]AGC 2742 GCTCAGC BmyI 1419 GTGCAC G[AGT]GC[ACT]^C 2740 GTGCTC Bpu1102I 1605 GCTTAGC GC^T[ACGT]AGC 2742 GCTCAGC BpuAI 2891 GAAGAC GAAGAC(2/6) 2989 GTCTTC BsaI 1304 GAGACC GGTCTC(1/5) 487 GGTCTC BsgI 1907 CTGCAC GTGCAG(16/14) 2129 GTGCAG BsiHKAI 1419 GTGCAC G[AT]GC[AT]^C 2740 GTGCTC BsmI 1350 GCATTC GAATGC(1/-1) 2254 GCATTC Bsp1286I 1419 GTGCAC G[AGT]GC[ACT]^C 2740 GTGCTC BspLU11I 2175 ACATGT A^CATGT 2277 ACATGT BspMI 1307 ACCTGC ACCTGC(4/8) 1905 ACCTGC BstAPI 1825 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2131 GCAGCCCATGC BstXI 1689 CCAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2594 CCAAGTGTATGG CelII 1605 GCTTAGC GC^T[ACGT]AGC 2742 GCTCAGC Cfr13I 1889 GGGCC G^G[ACGT]CC 2582 GGGCC DsaI 1742 CCATGG C^C[GA][CT]GG 2676 CCATGG EarI 2486 CTCTTC CTCTTC(1/4) 76 GAAGAG EcoNI 145 CCTTAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2748 CCTCCCCAAGG HpaI 1733 GTTAAC GTT^AAC 2019 GTTAAC Ksp632I 2486 CTCTTC CTCTTC(1/4) 76 GAAGAG NcoI 1742 CCATGG C^CATGG 2676 CCATGG NdeI 603 CATATG CA^TATG 1843 CATATG NspI 2175 ACATGT [GA]CATG^[CT] 2277 ACATGT PshAI 2568 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2982 GACACGTGTC Sau96I 1889 GGGCC G^G[ACGT]CC 2582 GGGCC SfaNI 1747 GATGC GCATC(5/9) 1822 GATGC SmlI 172 CTTAAG C^T[CT][GA]AG 1320 CTTAAG SspI 1126 AATATT AAT^ATT 2922 AATATT TaqI 2241 TCGA T^CGA 2871 TCGA TthHB8I 2241 TCGA T^CGA 2871 TCGA XcmI 214 CCATGTGTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2792 CCAAGCCGGATATGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI ApoI BbvI BcnI BfaI BsaJI BseRI BsiYI BslI BsmAI BspHI BsrI BssKI BstF5I DdeI DpnII DraI EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspI MwoI NciI NdeII NlaIII NlaIV PleI RcaI RsaI Sau3AI ScaI ScrFI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI