##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BamHI BcgI
BciVI BfaI BfrI BlnI BseAI
BsiWI BspEI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I CpoI EagI
EclXI Eco47III Eco52I EcoT22I FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MfeI MluI MroI
MunI NheI NotI NruI NsiI
NspV PacI PflFI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 208 GTCTAC GT^[AC][GT]AC
AgeI 1975 ACCGGT A^CCGGT
AhdI 132 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 132 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2259 TGCGCA TGC^GCA
BbrPI 1616 CACGTG CAC^GTG
BclI 2170 TGATCA T^GATCA
BglI 1672 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2294 AGATCT A^GATCT
BsaBI 2072 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1266 CGGTCG CG[GA][CT]^CG
BsmI 252 GAATGC GAATGC(1/-1)
BspDI 1499 ATCGAT AT^CGAT
BssSI 1421 CACGAG CACGAG(-5/-1)
BstAPI 1984 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1624 GGTCACC G^GT[ACGT]ACC
BstPI 1624 GGTCACC G^GT[ACGT]ACC
ClaI 1499 ATCGAT AT^CGAT
DraI 752 TTTAAA TTT^AAA
DraIII 2116 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 132 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1624 GGTCACC G^GT[ACGT]ACC
EcoRI 194 GAATTC G^AATTC
EcoRV 1078 GATATC GAT^ATC
FbaI 2170 TGATCA T^GATCA
FspI 2259 TGCGCA TGC^GCA
MamI 2072 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2482 GCCGGC GCC^GGC
NcoI 1962 CCATGG C^CATGG
NdeI 1127 CATATG CA^TATG
NgoMIV 2482 GCCGGC G^CCGGC
NspI 1950 GCATGT [GA]CATG^[CT]
PaeR7I 2303 CTCGAG C^TCGAG
PinAI 1975 ACCGGT A^CCGGT
PmaCI 1616 CACGTG CAC^GTG
PmlI 1616 CACGTG CAC^GTG
PshAI 3009 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2732 GAAGAGC GCTCTTC(1/4)
SbfI 2603 CCTGCAGG CCTGCA^GG
SmaI 2144 CCCGGG CCC^GGG
Sse8387I 2603 CCTGCAGG CCTGCA^GG
SspI 454 AATATT AAT^ATT
StuI 1718 AGGCCT AGG^CCT
XhoI 2303 CTCGAG C^TCGAG
XmaCI 2144 CCCGGG C^CCGGG
XmaI 2144 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 824 GACGTC GACGT^C
3009 GACGTC
Alw44I 878 GTGCAC G^TGCAC
1418 GTGCAC
AocI 2155 CCTCAGG CC^T[ACGT]AGG
2660 CCTGAGG
ApaLI 878 GTGCAC G^TGCAC
1418 GTGCAC
BbeI 531 GGCGCC GGCGC^C
2485 GGCGCC
BbvCI 2951 CCTCAGC CCTCAGC(-5/-2)
1903 GCTGAGG
BlpI 589 GCTGAGC GC^T[ACGT]AGC
1792 GCTGAGC
Bpu1102I 589 GCTGAGC GC^T[ACGT]AGC
1792 GCTGAGC
BsaAI 1196 TACGTG [CT]AC^GT[GA]
1616 CACGTG
BsaI 1218 GAGACC GGTCTC(1/5)
2164 GGTCTC
BsmBI 1760 CGTCTC CGTCTC(1/5)
1274 GAGACG
Bsp1407I 1116 TGTACA T^GTACA
2943 TGTACA
BspMI 1611 ACCTGC ACCTGC(4/8)
3172 ACCTGC
BsrBI 1478 CCGCTC CCGCTC(-3/-3)
1832 GAGCGG
BsrDI 1576 CATTGC GCAATG(2/0)
2518 CATTGC
BsrGI 1116 TGTACA T^GTACA
2943 TGTACA
Bsu36I 2155 CCTCAGG CC^T[ACGT]AGG
2660 CCTGAGG
CelII 589 GCTGAGC GC^T[ACGT]AGC
1792 GCTGAGC
DrdI 2180 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2556 GACTGCTGGGTC
Eco81I 2155 CCTCAGG CC^T[ACGT]AGG
2660 CCTGAGG
EcoNI 1933 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2572 CCTGAATCAGG
EcoT14I 978 CCAAGG C^C[AT][AT]GG
1962 CCATGG
HaeII 531 GGCGCC [GA]GCGC^[CT]
2485 GGCGCC
KasI 531 GGCGCC G^GCGCC
2485 GGCGCC
NarI 531 GGCGCC GG^CGCC
2485 GGCGCC
PflMI 1852 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2365 CCAGAGTGTGG
PvuII 306 CAGCTG CAG^CTG
575 CAGCTG
SacI 1795 GAGCTC GAGCT^C
2858 GAGCTC
SfoI 531 GGCGCC GGC^GCC
2485 GGCGCC
SspBI 1116 TGTACA T^GTACA
2943 TGTACA
StyI 978 CCAAGG C^C[AT][AT]GG
1962 CCATGG
Van91I 1852 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2365 CCAGAGTGTGG
XcmI 856 CCAGGTTCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1204 CCACAGTGGCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII