##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbeI
BbvCI BcgI BfrI BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BspEI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I FseI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SphI Sse8387I SwaI
Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 1446 CGCG CG^CG
AflIII 997 ACATGT A^C[GA][CT]GT
AviII 2554 TGCGCA TGC^GCA
BamHI 1391 GGATCC G^GATCC
BciVI 2163 GTATCC GTATCC(6/5)
BglI 215 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2300 AGATCT A^GATCT
BsgI 1345 CTGCAC GTGCAG(16/14)
Bsp1407I 611 TGTACA T^GTACA
BspDI 1848 ATCGAT AT^CGAT
BspLU11I 997 ACATGT A^CATGT
BspMI 2601 GCAGGT ACCTGC(4/8)
BsrBI 325 GAGCGG CCGCTC(-3/-3)
BsrDI 704 GCAATG GCAATG(2/0)
BsrFI 876 GCCGGT [GA]^CCGG[CT]
BsrGI 611 TGTACA T^GTACA
BstEII 304 GGTGACC G^GT[ACGT]ACC
BstPI 304 GGTGACC G^GT[ACGT]ACC
BstUI 1446 CGCG CG^CG
Cfr10I 876 GCCGGT [GA]^CCGG[CT]
ClaI 1848 ATCGAT AT^CGAT
DraI 3128 TTTAAA TTT^AAA
DrdI 2389 GACGGGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1449 GAAGAG CTCTTC(1/4)
EcoNI 1395 CCTTTCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 304 GGTGACC G^GT[ACGT]ACC
EcoRV 893 GATATC GAT^ATC
EcoT22I 1583 ATGCAT ATGCA^T
FspI 2554 TGCGCA TGC^GCA
Ksp632I 1449 GAAGAG CTCTTC(1/4)
MvnI 1446 CGCG CG^CG
NheI 669 GCTAGC G^CTAGC
NsiI 1583 ATGCAT ATGCA^T
PshAI 1111 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 791 CAGCTG CAG^CTG
SacI 93 GAGCTC GAGCT^C
ScaI 2961 AGTACT AGT^ACT
SmaI 363 CCCGGG CCC^GGG
SnaBI 2123 TACGTA TAC^GTA
SpeI 3067 ACTAGT A^CTAGT
SspBI 611 TGTACA T^GTACA
XmaCI 363 CCCGGG C^CCGGG
XmaI 363 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1646 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
2176 CAGGGCCTG
AocI 99 CCTGAGG CC^T[ACGT]AGG
2240 CCTCAGG
AspHI 93 GAGCTC G[AT]GC[AT]^C
1012 GAGCAC
BanI 2491 GGTGCC G^G[CT][GA]CC
3173 GGTGCC
BbrPI 1928 CACGTG CAC^GTG
3077 CACGTG
BclI 1096 TGATCA T^GATCA
1283 TGATCA
BsaWI 130 ACCGGA [AT]^CCGG[AT]
1250 TCCGGT
BsiHKAI 93 GAGCTC G[AT]GC[AT]^C
1012 GAGCAC
Bsu36I 99 CCTGAGG CC^T[ACGT]AGG
2240 CCTCAGG
Eco81I 99 CCTGAGG CC^T[ACGT]AGG
2240 CCTCAGG
EcoRI 1050 GAATTC G^AATTC
1672 GAATTC
FbaI 1096 TGATCA T^GATCA
1283 TGATCA
HaeII 709 GGCGCT [GA]GCGC^[CT]
2669 AGCGCC
HindIII 868 AAGCTT A^AGCTT
3256 AAGCTT
MspA1I 791 CAGCTG C[AC]G^C[GT]G
812 CAGCGG
NcoI 237 CCATGG C^CATGG
717 CCATGG
PaeR7I 317 CTCGAG C^TCGAG
985 CTCGAG
PmaCI 1928 CACGTG CAC^GTG
3077 CACGTG
PmlI 1928 CACGTG CAC^GTG
3077 CACGTG
PpuMI 2307 AGGACCC [GA]G^G[AT]CC[CT]
2740 AGGTCCC
PstI 150 CTGCAG CTGCA^G
809 CTGCAG
SfaNI 1236 GATGC GCATC(5/9)
1951 GCATC
StuI 103 AGGCCT AGG^CCT
1957 AGGCCT
XhoI 317 CTCGAG C^TCGAG
985 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI Asp700I AvaI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI