################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccII AccIII AclI AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AspI AvrII BbeI BbrPI BglI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI FseI HaeII HindIII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 1729 GGTACC G^GTACC AcyI 1013 GACGCC G[GA]^CG[CT]C AflII 2443 CTTAAG C^TTAAG AhdI 361 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1593 GGGCCC GGGCC^C AseI 3477 ATTAAT AT^TAAT AsnI 3477 ATTAAT AT^TAAT Asp700I 2683 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1729 GGTACC G^GTACC AspEI 361 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1743 GAGCAC G[AT]GC[AT]^C AviII 1411 TGCGCA TGC^GCA BalI 1979 TGGCCA TGG^CCA BamHI 373 GGATCC G^GATCC BbsI 1960 GAAGAC GAAGAC(2/6) BbvCI 1069 CCTCAGC CCTCAGC(-5/-2) BcgI 1525 CGAAATTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1941 GGATAC GTATCC(6/5) BfrI 2443 CTTAAG C^TTAAG BpuAI 1960 GAAGAC GAAGAC(2/6) BsaBI 2771 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1013 GACGCC G[GA]^CG[CT]C BsgI 115 CTGCAC GTGCAG(16/14) BsiHKAI 1743 GAGCAC G[AT]GC[AT]^C BsmI 1972 GAATGC GAATGC(1/-1) BspMI 977 GCAGGT ACCTGC(4/8) BsrBI 1838 GAGCGG CCGCTC(-3/-3) BstBI 726 TTCGAA TT^CGAA DsaI 1967 CCATGG C^C[GA][CT]GG EaeI 1979 TGGCCA [CT]^GGCC[GA] Eam1105I 361 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2261 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1003 GATATC GAT^ATC EcoT22I 1492 ATGCAT ATGCA^T FspI 1411 TGCGCA TGC^GCA HgaI 1013 GACGC GACGC(5/10) Hin1I 1013 GACGCC G[GA]^CG[CT]C KpnI 1729 GGTACC GGTAC^C MaeII 435 ACGT A^CGT MamI 2771 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1979 TGGCCA TGG^CCA MscI 1979 TGGCCA TGG^CCA MspA1I 1072 CAGCTG C[AC]G^C[GT]G NcoI 1967 CCATGG C^CATGG NsiI 1492 ATGCAT ATGCA^T NspI 1494 GCATGC [GA]CATG^[CT] NspV 726 TTCGAA TT^CGAA PacI 3281 TTAATTAA TTAAT^TAA PflMI 1766 CCACCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 3477 ATTAAT AT^TAAT PvuII 1072 CAGCTG CAG^CTG SapI 497 GAAGAGC GCTCTTC(1/4) ScaI 2231 AGTACT AGT^ACT SfuI 726 TTCGAA TT^CGAA SmaI 76 CCCGGG CCC^GGG SmlI 2443 CTTAAG C^T[CT][GA]AG SphI 1494 GCATGC GCATG^C StuI 1439 AGGCCT AGG^CCT Van91I 1766 CCACCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1639 TCTAGA T^CTAGA XmaCI 76 CCCGGG C^CCGGG XmaI 76 CCCGGG C^CCGGG XmnI 2683 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 1542 GTCTAC GT^[AC][GT]AC 2170 GTAGAC AlwNI 2643 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 2687 CAGTTCCTG AvaI 44 CTCGGG C^[CT]CG[GA]G 76 CCCGGG BanII 1593 GGGCCC G[GA]GC[CT]^C 3313 GGGCTC BglII 2109 AGATCT A^GATCT 2495 AGATCT BsmFI 2448 GGGAC GGGAC(10/14) 34 GTCCC BsoBI 44 CTCGGG C^[CT]CG[GA]G 76 CCCGGG BspHI 752 TCATGA T^CATGA 1372 TCATGA BstAPI 565 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2642 GCAGAGACTGC CfoI 1412 GCGC GCG^C 3417 GCGC HhaI 1412 GCGC GCG^C 3417 GCGC HinP1I 1412 GCGC G^CGC 3417 GCGC HincII 243 GTTGAC GT[CT]^[GA]AC 2464 GTTGAC HindII 243 GTTGAC GT[CT]^[GA]AC 2464 GTTGAC PleI 141 GACTC GAGTC(4/5) 1111 GAGTC PpuMI 339 AGGACCC [GA]G^G[AT]CC[CT] 1335 AGGACCC RcaI 752 TCATGA T^CATGA 1372 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BanI BbvI BclI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII