##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AspI AvrII BbeI BbrPI
BglI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI FseI HaeII
HindIII HpaI KasI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 1729 GGTACC G^GTACC
AcyI 1013 GACGCC G[GA]^CG[CT]C
AflII 2443 CTTAAG C^TTAAG
AhdI 361 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1593 GGGCCC GGGCC^C
AseI 3477 ATTAAT AT^TAAT
AsnI 3477 ATTAAT AT^TAAT
Asp700I 2683 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1729 GGTACC G^GTACC
AspEI 361 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1743 GAGCAC G[AT]GC[AT]^C
AviII 1411 TGCGCA TGC^GCA
BalI 1979 TGGCCA TGG^CCA
BamHI 373 GGATCC G^GATCC
BbsI 1960 GAAGAC GAAGAC(2/6)
BbvCI 1069 CCTCAGC CCTCAGC(-5/-2)
BcgI 1525 CGAAATTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1941 GGATAC GTATCC(6/5)
BfrI 2443 CTTAAG C^TTAAG
BpuAI 1960 GAAGAC GAAGAC(2/6)
BsaBI 2771 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1013 GACGCC G[GA]^CG[CT]C
BsgI 115 CTGCAC GTGCAG(16/14)
BsiHKAI 1743 GAGCAC G[AT]GC[AT]^C
BsmI 1972 GAATGC GAATGC(1/-1)
BspMI 977 GCAGGT ACCTGC(4/8)
BsrBI 1838 GAGCGG CCGCTC(-3/-3)
BstBI 726 TTCGAA TT^CGAA
DsaI 1967 CCATGG C^C[GA][CT]GG
EaeI 1979 TGGCCA [CT]^GGCC[GA]
Eam1105I 361 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2261 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1003 GATATC GAT^ATC
EcoT22I 1492 ATGCAT ATGCA^T
FspI 1411 TGCGCA TGC^GCA
HgaI 1013 GACGC GACGC(5/10)
Hin1I 1013 GACGCC G[GA]^CG[CT]C
KpnI 1729 GGTACC GGTAC^C
MaeII 435 ACGT A^CGT
MamI 2771 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1979 TGGCCA TGG^CCA
MscI 1979 TGGCCA TGG^CCA
MspA1I 1072 CAGCTG C[AC]G^C[GT]G
NcoI 1967 CCATGG C^CATGG
NsiI 1492 ATGCAT ATGCA^T
NspI 1494 GCATGC [GA]CATG^[CT]
NspV 726 TTCGAA TT^CGAA
PacI 3281 TTAATTAA TTAAT^TAA
PflMI 1766 CCACCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3477 ATTAAT AT^TAAT
PvuII 1072 CAGCTG CAG^CTG
SapI 497 GAAGAGC GCTCTTC(1/4)
ScaI 2231 AGTACT AGT^ACT
SfuI 726 TTCGAA TT^CGAA
SmaI 76 CCCGGG CCC^GGG
SmlI 2443 CTTAAG C^T[CT][GA]AG
SphI 1494 GCATGC GCATG^C
StuI 1439 AGGCCT AGG^CCT
Van91I 1766 CCACCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1639 TCTAGA T^CTAGA
XmaCI 76 CCCGGG C^CCGGG
XmaI 76 CCCGGG C^CCGGG
XmnI 2683 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1542 GTCTAC GT^[AC][GT]AC
2170 GTAGAC
AlwNI 2643 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2687 CAGTTCCTG
AvaI 44 CTCGGG C^[CT]CG[GA]G
76 CCCGGG
BanII 1593 GGGCCC G[GA]GC[CT]^C
3313 GGGCTC
BglII 2109 AGATCT A^GATCT
2495 AGATCT
BsmFI 2448 GGGAC GGGAC(10/14)
34 GTCCC
BsoBI 44 CTCGGG C^[CT]CG[GA]G
76 CCCGGG
BspHI 752 TCATGA T^CATGA
1372 TCATGA
BstAPI 565 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2642 GCAGAGACTGC
CfoI 1412 GCGC GCG^C
3417 GCGC
HhaI 1412 GCGC GCG^C
3417 GCGC
HinP1I 1412 GCGC G^CGC
3417 GCGC
HincII 243 GTTGAC GT[CT]^[GA]AC
2464 GTTGAC
HindII 243 GTTGAC GT[CT]^[GA]AC
2464 GTTGAC
PleI 141 GACTC GAGTC(4/5)
1111 GAGTC
PpuMI 339 AGGACCC [GA]G^G[AT]CC[CT]
1335 AGGACCC
RcaI 752 TCATGA T^CATGA
1372 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbvI BclI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII