##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII BamHI BbrPI BciVI
BclI BfrI BglII BsaAI BsaBI
BsaWI BseAI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MamI
MfeI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SapI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 1809 GAATTC [GA]^AATT[CT]
AflIII 1890 ACGCGT A^C[GA][CT]GT
AlwNI 2319 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1809 GAATTC [GA]^AATT[CT]
Asp700I 76 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1476 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1240 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiWI 2813 CGTACG C^GTACG
BsmBI 1647 GAGACG CGTCTC(1/5)
BsmI 2384 GAATGC GAATGC(1/-1)
BssSI 2748 CACGAG CACGAG(-5/-1)
DrdI 218 GACCGGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1809 GAATTC G^AATTC
HincII 2355 GTCGAC GT[CT]^[GA]AC
HindII 2355 GTCGAC GT[CT]^[GA]AC
MluI 1890 ACGCGT A^CGCGT
MseI 3095 TTAA T^TAA
MslI 2441 CACAGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 600 CCATGG C^CATGG
PaeR7I 2024 CTCGAG C^TCGAG
PflFI 1476 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 255 CAGCTG CAG^CTG
SalI 2355 GTCGAC G^TCGAC
SbfI 2408 CCTGCAGG CCTGCA^GG
SfaNI 789 GCATC GCATC(5/9)
SfiI 166 GGCCCCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1228 CCCGGG CCC^GGG
Sse8387I 2408 CCTGCAGG CCTGCA^GG
Tru9I 3095 TTAA T^TAA
Tth111I 1476 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2024 CTCGAG C^TCGAG
XmaCI 1228 CCCGGG C^CCGGG
XmaI 1228 CCCGGG C^CCGGG
XmnI 76 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 505 GGGCCC GGGCC^C
2191 GGGCCC
AvrII 2051 CCTAGG C^CTAGG
2057 CCTAGG
BlnI 2051 CCTAGG C^CTAGG
2057 CCTAGG
BspHI 1695 TCATGA T^CATGA
1986 TCATGA
BsrDI 1191 GCAATG GCAATG(2/0)
1870 GCAATG
BsrI 143 CCAGT ACTGG(1/-1)
2694 CCAGT
BstAPI 1539 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2318 GCAGAAGCTGC
BstEII 244 GGTAACC G^GT[ACGT]ACC
2414 GGTGACC
BstPI 244 GGTAACC G^GT[ACGT]ACC
2414 GGTGACC
BstYI 102 AGATCC [GA]^GATC[CT]
2425 AGATCC
CpoI 486 CGGTCCG CG^G[AT]CCG
2250 CGGTCCG
EcoO65I 244 GGTAACC G^GT[ACGT]ACC
2414 GGTGACC
KspI 928 CCGCGG CCGC^GG
2911 CCGCGG
MflI 102 AGATCC [GA]^GATC[CT]
2425 AGATCC
RcaI 1695 TCATGA T^CATGA
1986 TCATGA
RsrII 486 CGGTCCG CG^G[AT]CCG
2250 CGGTCCG
SacII 928 CCGCGG CCGC^GG
2911 CCGCGG
XhoII 102 AGATCC [GA]^GATC[CT]
2425 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BssKI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfcI
SfoI SmlI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI