################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp700I AspEI AspHI AspI AvrII BbeI BbsI BcgI BfrI BlnI BlpI Bpu1102I BpuAI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BspDI BspEI BspLU11I BsrFI BssHII BstAPI BstBI CelII Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I FseI HincII HindII HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 1908 GGTACC G^GTACC AccI 3207 GTATAC GT^[AC][GT]AC AcyI 1075 GGCGTC G[GA]^CG[CT]C AocI 1475 CCTGAGG CC^T[ACGT]AGG ApaI 1239 GGGCCC GGGCC^C AseI 602 ATTAAT AT^TAAT AsnI 602 ATTAAT AT^TAAT Asp718I 1908 GGTACC G^GTACC AvaI 1547 CTCGGG C^[CT]CG[GA]G AviII 159 TGCGCA TGC^GCA BbrPI 1034 CACGTG CAC^GTG BclI 2459 TGATCA T^GATCA BglI 1672 GCCTTTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 345 AGATCT A^GATCT BsaAI 1034 CACGTG [CT]AC^GT[GA] BsaHI 1075 GGCGTC G[GA]^CG[CT]C BsmBI 1077 CGTCTC CGTCTC(1/5) BsoBI 1547 CTCGGG C^[CT]CG[GA]G Bsp1407I 1734 TGTACA T^GTACA BsrGI 1734 TGTACA T^GTACA BssSI 2037 CACGAG CACGAG(-5/-1) Bst1107I 3207 GTATAC GTA^TAC BstEII 740 GGTGACC G^GT[ACGT]ACC BstPI 740 GGTGACC G^GT[ACGT]ACC BstZ17I 3207 GTATAC GTA^TAC Bsu36I 1475 CCTGAGG CC^T[ACGT]AGG DrdI 1324 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1475 CCTGAGG CC^T[ACGT]AGG EcoO65I 740 GGTGACC G^GT[ACGT]ACC EcoRI 2997 GAATTC G^AATTC EcoRV 2416 GATATC GAT^ATC FbaI 2459 TGATCA T^GATCA FspI 159 TGCGCA TGC^GCA Hin1I 1075 GGCGTC G[GA]^CG[CT]C HindIII 243 AAGCTT A^AGCTT KpnI 1908 GGTACC GGTAC^C PmaCI 1034 CACGTG CAC^GTG PmlI 1034 CACGTG CAC^GTG PshBI 602 ATTAAT AT^TAAT ScaI 2946 AGTACT AGT^ACT SspBI 1734 TGTACA T^GTACA StuI 757 AGGCCT AGG^CCT TaqI 1614 TCGA T^CGA TthHB8I 1614 TCGA T^CGA XbaI 2797 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 10 CGCG CG^CG 1175 CGCG BalI 1111 TGGCCA TGG^CCA 1584 TGGCCA BbvCI 62 GCTGAGG CCTCAGC(-5/-2) 1249 GCTGAGG BsgI 53 GTGCAG GTGCAG(16/14) 3080 GTGCAG BspMI 1158 ACCTGC ACCTGC(4/8) 1949 GCAGGT BsrDI 1020 CATTGC GCAATG(2/0) 654 GCAATG BstUI 10 CGCG CG^CG 1175 CGCG BstXI 1791 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2596 CCAAGATTTTGG DraIII 389 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 434 CACTCTGTG EaeI 1111 TGGCCA [CT]^GGCC[GA] 1584 TGGCCA HaeII 1376 GGCGCT [GA]GCGC^[CT] 1489 GGCGCT HgaI 130 GACGC GACGC(5/10) 1076 GCGTC MluNI 1111 TGGCCA TGG^CCA 1584 TGGCCA MscI 1111 TGGCCA TGG^CCA 1584 TGGCCA MvnI 10 CGCG CG^CG 1175 CGCG PpuMI 1155 AGGACCT [GA]G^G[AT]CC[CT] 1253 AGGACCT SapI 1186 GCTCTTC GCTCTTC(1/4) 3186 GCTCTTC SmlI 2100 CTCAAG C^T[CT][GA]AG 3214 CTTGAG SphI 1624 GCATGC GCATG^C 3022 GCATGC XcmI 1192 CCAGGTCATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2862 CCAATCTACAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BamHI BanI BanII BbvI BciVI BcnI BfaI BmyI BpmI BsaBI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII