##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp700I AspEI AspHI AspI
AvrII BbeI BbsI BcgI BfrI
BlnI BlpI Bpu1102I BpuAI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BspDI BspEI BspLU11I BsrFI BssHII
BstAPI BstBI CelII Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I FseI HincII HindII HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1908 GGTACC G^GTACC
AccI 3207 GTATAC GT^[AC][GT]AC
AcyI 1075 GGCGTC G[GA]^CG[CT]C
AocI 1475 CCTGAGG CC^T[ACGT]AGG
ApaI 1239 GGGCCC GGGCC^C
AseI 602 ATTAAT AT^TAAT
AsnI 602 ATTAAT AT^TAAT
Asp718I 1908 GGTACC G^GTACC
AvaI 1547 CTCGGG C^[CT]CG[GA]G
AviII 159 TGCGCA TGC^GCA
BbrPI 1034 CACGTG CAC^GTG
BclI 2459 TGATCA T^GATCA
BglI 1672 GCCTTTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 345 AGATCT A^GATCT
BsaAI 1034 CACGTG [CT]AC^GT[GA]
BsaHI 1075 GGCGTC G[GA]^CG[CT]C
BsmBI 1077 CGTCTC CGTCTC(1/5)
BsoBI 1547 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1734 TGTACA T^GTACA
BsrGI 1734 TGTACA T^GTACA
BssSI 2037 CACGAG CACGAG(-5/-1)
Bst1107I 3207 GTATAC GTA^TAC
BstEII 740 GGTGACC G^GT[ACGT]ACC
BstPI 740 GGTGACC G^GT[ACGT]ACC
BstZ17I 3207 GTATAC GTA^TAC
Bsu36I 1475 CCTGAGG CC^T[ACGT]AGG
DrdI 1324 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1475 CCTGAGG CC^T[ACGT]AGG
EcoO65I 740 GGTGACC G^GT[ACGT]ACC
EcoRI 2997 GAATTC G^AATTC
EcoRV 2416 GATATC GAT^ATC
FbaI 2459 TGATCA T^GATCA
FspI 159 TGCGCA TGC^GCA
Hin1I 1075 GGCGTC G[GA]^CG[CT]C
HindIII 243 AAGCTT A^AGCTT
KpnI 1908 GGTACC GGTAC^C
PmaCI 1034 CACGTG CAC^GTG
PmlI 1034 CACGTG CAC^GTG
PshBI 602 ATTAAT AT^TAAT
ScaI 2946 AGTACT AGT^ACT
SspBI 1734 TGTACA T^GTACA
StuI 757 AGGCCT AGG^CCT
TaqI 1614 TCGA T^CGA
TthHB8I 1614 TCGA T^CGA
XbaI 2797 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 10 CGCG CG^CG
1175 CGCG
BalI 1111 TGGCCA TGG^CCA
1584 TGGCCA
BbvCI 62 GCTGAGG CCTCAGC(-5/-2)
1249 GCTGAGG
BsgI 53 GTGCAG GTGCAG(16/14)
3080 GTGCAG
BspMI 1158 ACCTGC ACCTGC(4/8)
1949 GCAGGT
BsrDI 1020 CATTGC GCAATG(2/0)
654 GCAATG
BstUI 10 CGCG CG^CG
1175 CGCG
BstXI 1791 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2596 CCAAGATTTTGG
DraIII 389 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
434 CACTCTGTG
EaeI 1111 TGGCCA [CT]^GGCC[GA]
1584 TGGCCA
HaeII 1376 GGCGCT [GA]GCGC^[CT]
1489 GGCGCT
HgaI 130 GACGC GACGC(5/10)
1076 GCGTC
MluNI 1111 TGGCCA TGG^CCA
1584 TGGCCA
MscI 1111 TGGCCA TGG^CCA
1584 TGGCCA
MvnI 10 CGCG CG^CG
1175 CGCG
PpuMI 1155 AGGACCT [GA]G^G[AT]CC[CT]
1253 AGGACCT
SapI 1186 GCTCTTC GCTCTTC(1/4)
3186 GCTCTTC
SmlI 2100 CTCAAG C^T[CT][GA]AG
3214 CTTGAG
SphI 1624 GCATGC GCATG^C
3022 GCATGC
XcmI 1192 CCAGGTCATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2862 CCAATCTACAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BamHI BanI
BanII BbvI BciVI BcnI BfaI
BmyI BpmI BsaBI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII