################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII AccII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspHI AviII AvrII BalI BbrPI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI CelII ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FbaI FseI FspI HindIII KspI MamI MfeI MluI MluNI MscI MunI MvnI NdeI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SphI Sse8387I SspBI SwaI XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 1351 GGTACC G^GTACC AccIII 700 TCCGGA T^CCGGA AclI 2145 AACGTT AA^CGTT AflII 2257 CTTAAG C^TTAAG AocI 9 CCTCAGG CC^T[ACGT]AGG AseI 1300 ATTAAT AT^TAAT AsnI 1300 ATTAAT AT^TAAT Asp700I 1376 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1351 GGTACC G^GTACC AspI 2455 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2522 CTCGAG C^[CT]CG[GA]G BamHI 697 GGATCC G^GATCC BbeI 672 GGCGCC GGCGC^C BbsI 1199 GAAGAC GAAGAC(2/6) BbvCI 1060 CCTCAGC CCTCAGC(-5/-2) BcgI 2514 GCAGCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2257 CTTAAG C^TTAAG BpuAI 1199 GAAGAC GAAGAC(2/6) BsaWI 700 TCCGGA [AT]^CCGG[AT] BseAI 700 TCCGGA T^CCGGA BsoBI 2522 CTCGAG C^[CT]CG[GA]G BspEI 700 TCCGGA T^CCGGA BspMI 2910 GCAGGT ACCTGC(4/8) Bst1107I 1475 GTATAC GTA^TAC BstZ17I 1475 GTATAC GTA^TAC Bsu36I 9 CCTCAGG CC^T[ACGT]AGG CfoI 673 GCGC GCG^C DraII 122 GGGACCC [GA]G^G[ACGT]CC[CT] Eco81I 9 CCTCAGG CC^T[ACGT]AGG EcoNI 47 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 122 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 936 GATATC GAT^ATC EcoT22I 263 ATGCAT ATGCA^T HaeII 672 GGCGCC [GA]GCGC^[CT] HhaI 673 GCGC GCG^C HinP1I 673 GCGC G^CGC HincII 886 GTTAAC GT[CT]^[GA]AC HindII 886 GTTAAC GT[CT]^[GA]AC HpaI 886 GTTAAC GTT^AAC KasI 672 GGCGCC G^GCGCC KpnI 1351 GGTACC GGTAC^C MroI 700 TCCGGA T^CCGGA NaeI 675 GCCGGC GCC^GGC NarI 672 GGCGCC GG^CGCC NgoMIV 675 GCCGGC G^CCGGC NsiI 263 ATGCAT ATGCA^T PaeR7I 2522 CTCGAG C^TCGAG PflFI 2455 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1849 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 122 GGGACCC [GA]G^G[AT]CC[CT] PshAI 180 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1300 ATTAAT AT^TAAT Psp1406I 2145 AACGTT AA^CGTT SapI 1135 GAAGAGC GCTCTTC(1/4) SfoI 672 GGCGCC GGC^GCC SgrAI 674 CGCCGGCG C[GA]^CCGG[CT]G SpeI 3151 ACTAGT A^CTAGT StuI 44 AGGCCT AGG^CCT Tsp45I 1564 GTCAC ^GT[GC]AC Tth111I 2455 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1849 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2522 CTCGAG C^TCGAG XmnI 1376 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 689 GTAGAC GT^[AC][GT]AC 1475 GTATAC AcyI 385 GACGCC G[GA]^CG[CT]C 672 GGCGCC AlwNI 425 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 2911 CAGGTGCTG BanI 672 GGCGCC G^G[CT][GA]CC 1351 GGTACC BanII 367 GGGCTC G[GA]GC[CT]^C 2182 GGGCTC BcnI 372 CCCGG CC^[GC]GG 736 CCGGG BsaHI 385 GACGCC G[GA]^CG[CT]C 672 GGCGCC BsaI 2657 GGTCTC GGTCTC(1/5) 3126 GGTCTC BsmI 2937 GAATGC GAATGC(1/-1) 265 GCATTC BsrDI 1794 CATTGC GCAATG(2/0) 1867 CATTGC EcoRI 19 GAATTC G^AATTC 1571 GAATTC FauI 412 CCCGC CCCGC(4/6) 750 CCCGC HgaI 385 GACGC GACGC(5/10) 1155 GCGTC Hin1I 385 GACGCC G[GA]^CG[CT]C 672 GGCGCC MaeII 1854 ACGT A^CGT 2146 ACGT NciI 372 CCCGG CC^[GC]GG 736 CCGGG PstI 754 CTGCAG CTGCA^G 2509 CTGCAG SmlI 2257 CTTAAG C^T[CT][GA]AG 2522 CTCGAG SspI 1624 AATATT AAT^ATT 3190 AATATT TaqI 2523 TCGA T^CGA 3130 TCGA TthHB8I 2523 TCGA T^CGA 3130 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII