##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspHI AviII AvrII BalI
BbrPI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI CelII ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FbaI
FseI FspI HindIII KspI MamI
MfeI MluI MluNI MscI MunI
MvnI NdeI NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SphI
Sse8387I SspBI SwaI XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 1351 GGTACC G^GTACC
AccIII 700 TCCGGA T^CCGGA
AclI 2145 AACGTT AA^CGTT
AflII 2257 CTTAAG C^TTAAG
AocI 9 CCTCAGG CC^T[ACGT]AGG
AseI 1300 ATTAAT AT^TAAT
AsnI 1300 ATTAAT AT^TAAT
Asp700I 1376 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1351 GGTACC G^GTACC
AspI 2455 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2522 CTCGAG C^[CT]CG[GA]G
BamHI 697 GGATCC G^GATCC
BbeI 672 GGCGCC GGCGC^C
BbsI 1199 GAAGAC GAAGAC(2/6)
BbvCI 1060 CCTCAGC CCTCAGC(-5/-2)
BcgI 2514 GCAGCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2257 CTTAAG C^TTAAG
BpuAI 1199 GAAGAC GAAGAC(2/6)
BsaWI 700 TCCGGA [AT]^CCGG[AT]
BseAI 700 TCCGGA T^CCGGA
BsoBI 2522 CTCGAG C^[CT]CG[GA]G
BspEI 700 TCCGGA T^CCGGA
BspMI 2910 GCAGGT ACCTGC(4/8)
Bst1107I 1475 GTATAC GTA^TAC
BstZ17I 1475 GTATAC GTA^TAC
Bsu36I 9 CCTCAGG CC^T[ACGT]AGG
CfoI 673 GCGC GCG^C
DraII 122 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 9 CCTCAGG CC^T[ACGT]AGG
EcoNI 47 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 122 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 936 GATATC GAT^ATC
EcoT22I 263 ATGCAT ATGCA^T
HaeII 672 GGCGCC [GA]GCGC^[CT]
HhaI 673 GCGC GCG^C
HinP1I 673 GCGC G^CGC
HincII 886 GTTAAC GT[CT]^[GA]AC
HindII 886 GTTAAC GT[CT]^[GA]AC
HpaI 886 GTTAAC GTT^AAC
KasI 672 GGCGCC G^GCGCC
KpnI 1351 GGTACC GGTAC^C
MroI 700 TCCGGA T^CCGGA
NaeI 675 GCCGGC GCC^GGC
NarI 672 GGCGCC GG^CGCC
NgoMIV 675 GCCGGC G^CCGGC
NsiI 263 ATGCAT ATGCA^T
PaeR7I 2522 CTCGAG C^TCGAG
PflFI 2455 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1849 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 122 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 180 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1300 ATTAAT AT^TAAT
Psp1406I 2145 AACGTT AA^CGTT
SapI 1135 GAAGAGC GCTCTTC(1/4)
SfoI 672 GGCGCC GGC^GCC
SgrAI 674 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3151 ACTAGT A^CTAGT
StuI 44 AGGCCT AGG^CCT
Tsp45I 1564 GTCAC ^GT[GC]AC
Tth111I 2455 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1849 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2522 CTCGAG C^TCGAG
XmnI 1376 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 689 GTAGAC GT^[AC][GT]AC
1475 GTATAC
AcyI 385 GACGCC G[GA]^CG[CT]C
672 GGCGCC
AlwNI 425 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
2911 CAGGTGCTG
BanI 672 GGCGCC G^G[CT][GA]CC
1351 GGTACC
BanII 367 GGGCTC G[GA]GC[CT]^C
2182 GGGCTC
BcnI 372 CCCGG CC^[GC]GG
736 CCGGG
BsaHI 385 GACGCC G[GA]^CG[CT]C
672 GGCGCC
BsaI 2657 GGTCTC GGTCTC(1/5)
3126 GGTCTC
BsmI 2937 GAATGC GAATGC(1/-1)
265 GCATTC
BsrDI 1794 CATTGC GCAATG(2/0)
1867 CATTGC
EcoRI 19 GAATTC G^AATTC
1571 GAATTC
FauI 412 CCCGC CCCGC(4/6)
750 CCCGC
HgaI 385 GACGC GACGC(5/10)
1155 GCGTC
Hin1I 385 GACGCC G[GA]^CG[CT]C
672 GGCGCC
MaeII 1854 ACGT A^CGT
2146 ACGT
NciI 372 CCCGG CC^[GC]GG
736 CCGGG
PstI 754 CTGCAG CTGCA^G
2509 CTGCAG
SmlI 2257 CTTAAG C^T[CT][GA]AG
2522 CTCGAG
SspI 1624 AATATT AAT^ATT
3190 AATATT
TaqI 2523 TCGA T^CGA
3130 TCGA
TthHB8I 2523 TCGA T^CGA
3130 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII DraI DsaI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII