##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspEI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BglI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI FseI FspI HaeII Hin1I
KasI KspI MamI MluI MroI
NaeI NarI NcoI NgoMIV NotI
NruI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI SwaI
Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 2163 GGTACC G^GTACC
AccI 2888 GTAGAC GT^[AC][GT]AC
AccII 82 CGCG CG^CG
AclI 1849 AACGTT AA^CGTT
AflII 430 CTTAAG C^TTAAG
AseI 350 ATTAAT AT^TAAT
AsnI 350 ATTAAT AT^TAAT
Asp700I 959 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2163 GGTACC G^GTACC
AspI 3056 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 98 GGATCC G^GATCC
BclI 2169 TGATCA T^GATCA
BfrI 430 CTTAAG C^TTAAG
BlpI 2732 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2732 GCTGAGC GC^T[ACGT]AGC
BsmBI 136 GAGACG CGTCTC(1/5)
BspMI 1805 ACCTGC ACCTGC(4/8)
BstAPI 2585 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2846 GGTGACC G^GT[ACGT]ACC
BstPI 2846 GGTGACC G^GT[ACGT]ACC
BstUI 82 CGCG CG^CG
CelII 2732 GCTGAGC GC^T[ACGT]AGC
CfoI 83 GCGC GCG^C
DraIII 2841 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1861 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1385 CCGTGG C^C[GA][CT]GG
EcoO65I 2846 GGTGACC G^GT[ACGT]ACC
EcoRV 2684 GATATC GAT^ATC
EcoT14I 768 CCTTGG C^C[AT][AT]GG
EcoT22I 1839 ATGCAT ATGCA^T
FbaI 2169 TGATCA T^GATCA
HgaI 1633 GCGTC GACGC(5/10)
HhaI 83 GCGC GCG^C
HinP1I 83 GCGC G^CGC
HpaI 1801 GTTAAC GTT^AAC
KpnI 2163 GGTACC GGTAC^C
MvnI 82 CGCG CG^CG
NdeI 1402 CATATG CA^TATG
NheI 1744 GCTAGC G^CTAGC
NsiI 1839 ATGCAT ATGCA^T
PaeR7I 2826 CTCGAG C^TCGAG
PflFI 3056 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 350 ATTAAT AT^TAAT
Psp1406I 1849 AACGTT AA^CGTT
PvuII 1085 CAGCTG CAG^CTG
SacI 413 GAGCTC GAGCT^C
SapI 887 GAAGAGC GCTCTTC(1/4)
SexAI 2298 ACCTGGT A^CC[AT]GGT
SnaBI 3129 TACGTA TAC^GTA
StyI 768 CCTTGG C^C[AT][AT]GG
Tth111I 3056 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2826 CTCGAG C^TCGAG
XmnI 959 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaI 2143 CCCGAG C^[CT]CG[GA]G
2826 CTCGAG
BalI 771 TGGCCA TGG^CCA
1035 TGGCCA
BcnI 53 CCGGG CC^[GC]GG
2861 CCCGG
BsaAI 3077 TACGTG [CT]AC^GT[GA]
3129 TACGTA
BsaI 207 GAGACC GGTCTC(1/5)
2864 GGTCTC
BseRI 383 CTCCTC GAGGAG(10/8)
1126 GAGGAG
BsiYI 1872 CCCTGTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1959 CCATGTTTGGG
BslI 1872 CCCTGTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1959 CCATGTTTGGG
BsoBI 2143 CCCGAG C^[CT]CG[GA]G
2826 CTCGAG
BssSI 677 CACGAG CACGAG(-5/-1)
1554 CACGAG
BstXI 948 CCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1581 CCACTCTGGTGG
DraI 338 TTTAAA TTT^AAA
3166 TTTAAA
EaeI 771 TGGCCA [CT]^GGCC[GA]
1035 TGGCCA
FauI 69 CCCGC CCCGC(4/6)
80 CCCGC
HapII 53 CCGG C^CGG
2862 CCGG
HindIII 433 AAGCTT A^AGCTT
2084 AAGCTT
HpaII 53 CCGG C^CGG
2862 CCGG
MluNI 771 TGGCCA TGG^CCA
1035 TGGCCA
MscI 771 TGGCCA TGG^CCA
1035 TGGCCA
MspA1I 685 CCGCTG C[AC]G^C[GT]G
1085 CAGCTG
MspI 53 CCGG C^CGG
2862 CCGG
NciI 53 CCGGG CC^[GC]GG
2861 CCCGG
PshAI 983 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1912 GACCAATGTC
PstI 1865 CTGCAG CTGCA^G
2790 CTGCAG
StuI 765 AGGCCT AGG^CCT
2350 AGGCCT
XcmI 711 CCAGTTCAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1553 CCACGAGAATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRI EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII