##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspI AviII BalI BbrPI
BbsI BcgI BciVI BglII BlpI
Bpu1102I BpuAI BsaI BsiEI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BsrBI BssSI BstBI Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV FseI FspI KpnI MfeI
MluI MluNI MscI MunI NaeI
NcoI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SalI
SapI SbfI SexAI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
Tth111I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 652 TCCGGA T^CCGGA
AflII 2261 CTTAAG C^TTAAG
AhdI 2771 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1319 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2771 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1254 CTCGAG C^[CT]CG[GA]G
AvrII 114 CCTAGG C^CTAGG
BbvCI 235 GCTGAGG CCTCAGC(-5/-2)
BfrI 2261 CTTAAG C^TTAAG
BlnI 114 CCTAGG C^CTAGG
BsaAI 3282 CACGTA [CT]AC^GT[GA]
BsaWI 652 TCCGGA [AT]^CCGG[AT]
BseAI 652 TCCGGA T^CCGGA
BsoBI 1254 CTCGAG C^[CT]CG[GA]G
Bsp1407I 855 TGTACA T^GTACA
BspEI 652 TCCGGA T^CCGGA
BspMI 255 ACCTGC ACCTGC(4/8)
BsrFI 66 GCCGGT [GA]^CCGG[CT]
BsrGI 855 TGTACA T^GTACA
Bst1107I 1061 GTATAC GTA^TAC
BstAPI 1259 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 922 GGTAACC G^GT[ACGT]ACC
BstPI 922 GGTAACC G^GT[ACGT]ACC
BstZ17I 1061 GTATAC GTA^TAC
Cfr10I 66 GCCGGT [GA]^CCGG[CT]
EaeI 328 CGGCCA [CT]^GGCC[GA]
Eam1105I 2771 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1175 GAAGAG CTCTTC(1/4)
EcoNI 289 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 922 GGTAACC G^GT[ACGT]ACC
EcoT22I 2131 ATGCAT ATGCA^T
HpaI 3218 GTTAAC GTT^AAC
Ksp632I 1175 GAAGAG CTCTTC(1/4)
MroI 652 TCCGGA T^CCGGA
NdeI 1974 CATATG CA^TATG
NsiI 2131 ATGCAT ATGCA^T
PaeR7I 1254 CTCGAG C^TCGAG
SacI 3056 GAGCTC GAGCT^C
ScaI 1218 AGTACT AGT^ACT
SphI 2347 GCATGC GCATG^C
SspBI 855 TGTACA T^GTACA
SwaI 3313 ATTTAAAT ATTT^AAAT
XhoI 1254 CTCGAG C^TCGAG
XmnI 1319 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 340 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1260 CAGCTTCTG
ApaI 43 GGGCCC GGGCC^C
110 GGGCCC
AseI 2127 ATTAAT AT^TAAT
2789 ATTAAT
AsnI 2127 ATTAAT AT^TAAT
2789 ATTAAT
AspHI 900 GTGCTC G[AT]GC[AT]^C
3056 GAGCTC
BamHI 649 GGATCC G^GATCC
2335 GGATCC
BsaBI 175 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2336 GATCCTTATC
BsiHKAI 900 GTGCTC G[AT]GC[AT]^C
3056 GAGCTC
BsmAI 2784 GAGAC GTCTC(1/5)
664 GTCTC
BstXI 1270 CCACGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3145 CCAGATTCCTGG
HgaI 1 GACGC GACGC(5/10)
464 GACGC
HincII 395 GTCAAC GT[CT]^[GA]AC
3218 GTTAAC
HindII 395 GTCAAC GT[CT]^[GA]AC
3218 GTTAAC
HindIII 528 AAGCTT A^AGCTT
979 AAGCTT
MaeII 1562 ACGT A^CGT
3283 ACGT
MamI 175 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2336 GATCCTTATC
PshBI 2127 ATTAAT AT^TAAT
2789 ATTAAT
SfiI 26 GGCCAGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
35 GGCCGCCGGGGCC
StuI 2380 AGGCCT AGG^CCT
2602 AGGCCT
XbaI 2519 TCTAGA T^CTAGA
2780 TCTAGA
XcmI 2057 CCAGTGGTTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2321 CCAAAAATAATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AvaII
BanI BanII BbeI BbvI BclI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmFI Bsp1286I BsrDI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KspI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII