##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BfrI BglI
BlnI BpuAI BsaAI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspMI BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT14I
EcoT22I FseI FspI HaeII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StyI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2109 GACGTC GACGT^C
AccII 44 CGCG CG^CG
AcyI 2109 GACGTC G[GA]^CG[CT]C
AlwI 2960 GATCC GGATC(4/5)
AlwNI 268 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1686 CCTTAGG CC^T[ACGT]AGG
ApaI 554 GGGCCC GGGCC^C
AspHI 1762 GAGCAC G[AT]GC[AT]^C
AspI 3043 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2970 CCTCAGC CCTCAGC(-5/-2)
BclI 251 TGATCA T^GATCA
BcnI 17 CCCGG CC^[GC]GG
BlpI 35 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 35 GCTGAGC GC^T[ACGT]AGC
BsaHI 2109 GACGTC G[GA]^CG[CT]C
BsgI 2908 GTGCAG GTGCAG(16/14)
BsiHKAI 1762 GAGCAC G[AT]GC[AT]^C
BspHI 943 TCATGA T^CATGA
BsrBI 38 GAGCGG CCGCTC(-3/-3)
BsrDI 325 GCAATG GCAATG(2/0)
BstBI 232 TTCGAA TT^CGAA
BstUI 44 CGCG CG^CG
Bsu36I 1686 CCTTAGG CC^T[ACGT]AGG
CelII 35 GCTGAGC GC^T[ACGT]AGC
CfoI 43 GCGC GCG^C
DraIII 2411 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 61 CCGTGG C^C[GA][CT]GG
Eco81I 1686 CCTTAGG CC^T[ACGT]AGG
EcoRV 999 GATATC GAT^ATC
FauI 45 GCGGG CCCGC(4/6)
FbaI 251 TGATCA T^GATCA
HapII 18 CCGG C^CGG
HgaI 1512 GCGTC GACGC(5/10)
HhaI 43 GCGC GCG^C
Hin1I 2109 GACGTC G[GA]^CG[CT]C
HinP1I 43 GCGC G^CGC
HpaII 18 CCGG C^CGG
MspI 18 CCGG C^CGG
MvnI 44 CGCG CG^CG
NciI 17 CCCGG CC^[GC]GG
NheI 2347 GCTAGC G^CTAGC
NspV 232 TTCGAA TT^CGAA
PflFI 3043 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 540 CCAGATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 943 TCATGA T^CATGA
SapI 2829 GAAGAGC GCTCTTC(1/4)
SfuI 232 TTCGAA TT^CGAA
StuI 1690 AGGCCT AGG^CCT
Tth111I 3043 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 540 CCAGATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 52 CCAGACTCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 40 GCGG CCGC(-3/-1)
45 GCGG
AflIII 1270 ACATGT A^C[GA][CT]GT
2763 ACATGT
AvaII 1440 GGACC G^G[AT]CC
2632 GGACC
BciVI 3173 GGATAC GTATCC(6/5)
1626 GTATCC
BglII 1793 AGATCT A^GATCT
2207 AGATCT
BsaBI 165 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
252 GATCAATATC
BsaI 1724 GAGACC GGTCTC(1/5)
3268 GAGACC
BspLU11I 1270 ACATGT A^CATGT
2763 ACATGT
BssSI 2979 CACGAG CACGAG(-5/-1)
2414 CTCGTG
BstYI 1793 AGATCT [GA]^GATC[CT]
2207 AGATCT
HindIII 513 AAGCTT A^AGCTT
2616 AAGCTT
HpaI 708 GTTAAC GTT^AAC
1274 GTTAAC
MaeII 2110 ACGT A^CGT
2480 ACGT
MamI 165 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
252 GATCAATATC
MflI 1793 AGATCT [GA]^GATC[CT]
2207 AGATCT
MslI 2764 CATGTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2917 CACAGTTATG
MspA1I 187 CAGCTG C[AC]G^C[GT]G
477 CAGCTG
NdeI 2013 CATATG CA^TATG
2598 CATATG
NspI 1270 ACATGT [GA]CATG^[CT]
2763 ACATGT
PpuMI 1439 GGGACCT [GA]G^G[AT]CC[CT]
2631 AGGACCT
PstI 1965 CTGCAG CTGCA^G
2903 CTGCAG
PvuII 187 CAGCTG CAG^CTG
477 CAGCTG
XbaI 1664 TCTAGA T^CTAGA
2000 TCTAGA
XhoII 1793 AGATCT [GA]^GATC[CT]
2207 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI Asp700I BanII BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI