##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BanII BbeI BbrPI
BbsI BbvCI BglII BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstEII
BstPI Bsu36I CelII Cfr10I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HaeII Hin1I HpaI KasI
KpnI KspI MroI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SacII SalI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SphI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Asp700I 3213 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 949 CCCGAG C^[CT]CG[GA]G
BamHI 1097 GGATCC G^GATCC
BcgI 1004 GCATGAGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2187 GGATAC GTATCC(6/5)
BcnI 116 CCGGG CC^[GC]GG
BsaAI 903 TACGTG [CT]AC^GT[GA]
BsaWI 82 TCCGGT [AT]^CCGG[AT]
BsmI 908 GAATGC GAATGC(1/-1)
BsoBI 949 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3145 TGTACA T^GTACA
BspDI 3655 ATCGAT AT^CGAT
BspLU11I 3519 ACATGT A^CATGT
BsrGI 3145 TGTACA T^GTACA
BstAPI 585 GCACAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 102 GCGC GCG^C
ClaI 3655 ATCGAT AT^CGAT
DraI 3687 TTTAAA TTT^AAA
DraII 1359 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 3 GACGCGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 663 CCTGTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1359 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1711 GAATTC G^AATTC
FauI 104 GCGGG CCCGC(4/6)
HhaI 102 GCGC GCG^C
HinP1I 102 GCGC G^CGC
HincII 1869 GTTGAC GT[CT]^[GA]AC
HindII 1869 GTTGAC GT[CT]^[GA]AC
HindIII 2348 AAGCTT A^AGCTT
MaeII 904 ACGT A^CGT
MluI 4 ACGCGT A^CGCGT
NciI 116 CCGGG CC^[GC]GG
NdeI 1089 CATATG CA^TATG
PpuMI 1359 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 2427 CAGCTG CAG^CTG
SapI 891 GAAGAGC GCTCTTC(1/4)
SbfI 2537 CCTGCAGG CCTGCA^GG
SfiI 477 GGCCAGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3344 ACTAGT A^CTAGT
Sse8387I 2537 CCTGCAGG CCTGCA^GG
SspBI 3145 TGTACA T^GTACA
SspI 2853 AATATT AAT^ATT
StuI 2466 AGGCCT AGG^CCT
XmnI 3213 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 203 GTATAC GT^[AC][GT]AC
2145 GTATAC
AccII 5 CGCG CG^CG
103 CGCG
AflII 933 CTTAAG C^TTAAG
3096 CTTAAG
AflIII 4 ACGCGT A^C[GA][CT]GT
3519 ACATGT
AlwNI 658 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2547 CAGGAACTG
AspHI 706 GAGCAC G[AT]GC[AT]^C
2166 GAGCAC
AspI 361 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2325 GACAGAGTC
BalI 326 TGGCCA TGG^CCA
536 TGGCCA
BfrI 933 CTTAAG C^TTAAG
3096 CTTAAG
BglI 478 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
693 GCCAGTTTGGC
BmyI 706 GAGCAC G[AGT]GC[ACT]^C
2166 GAGCAC
BseRI 938 GAGGAG GAGGAG(10/8)
2000 GAGGAG
BsiHKAI 706 GAGCAC G[AT]GC[AT]^C
2166 GAGCAC
BsmAI 1416 GAGAC GTCTC(1/5)
1010 GTCTC
Bsp1286I 706 GAGCAC G[AGT]GC[ACT]^C
2166 GAGCAC
BspHI 817 TCATGA T^CATGA
3255 TCATGA
Bst1107I 203 GTATAC GTA^TAC
2145 GTATAC
BstUI 5 CGCG CG^CG
103 CGCG
BstXI 2866 CCAGGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3630 CCAGCAGTATGG
BstZ17I 203 GTATAC GTA^TAC
2145 GTATAC
DraIII 130 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3299 CACAAAGTG
EaeI 326 TGGCCA [CT]^GGCC[GA]
536 TGGCCA
HapII 83 CCGG C^CGG
116 CCGG
HgaI 3 GACGC GACGC(5/10)
1607 GACGC
HpaII 83 CCGG C^CGG
116 CCGG
MfeI 1301 CAATTG C^AATTG
3675 CAATTG
MluNI 326 TGGCCA TGG^CCA
536 TGGCCA
MscI 326 TGGCCA TGG^CCA
536 TGGCCA
MspI 83 CCGG C^CGG
116 CCGG
MunI 1301 CAATTG C^AATTG
3675 CAATTG
MvnI 5 CGCG CG^CG
103 CGCG
PflFI 361 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2325 GACAGAGTC
PshAI 521 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
605 GACCATCGTC
RcaI 817 TCATGA T^CATGA
3255 TCATGA
Tth111I 361 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2325 GACAGAGTC
XbaI 1553 TCTAGA T^CTAGA
2235 TCTAGA
XcmI 344 CCAGGCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
443 CCAGATCCAGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BanI
BbvI BclI BfaI BpmI BsaBI
BsaJI BsgI BsiYI BslI BsmFI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII