##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AflII AflIII AhdI ApaI
AscI AseI AsnI Asp718I AspEI
AvrII BfrI BglII BlnI BsaBI
BsiWI BsmI BspDI BspHI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraIII Eam1105I EcoRI EcoT22I
FseI HindIII KpnI MamI MfeI
MluI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PmeI
PshAI PshBI PvuI RcaI RsrII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SwaI Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1085 GACGTC GACGT^C
AccI 1820 GTCTAC GT^[AC][GT]AC
AccIII 881 TCCGGA T^CCGGA
AgeI 2545 ACCGGT A^CCGGT
Alw44I 978 GTGCAC G^TGCAC
AlwNI 488 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 397 AGCGCT AGC^GCT
ApaLI 978 GTGCAC G^TGCAC
Asp700I 656 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1629 TGCGCA TGC^GCA
BamHI 232 GGATCC G^GATCC
BbeI 1352 GGCGCC GGCGC^C
BbrPI 1733 CACGTG CAC^GTG
BbsI 1849 GTCTTC GAAGAC(2/6)
BglI 1325 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1849 GTCTTC GAAGAC(2/6)
BsaAI 1733 CACGTG [CT]AC^GT[GA]
BseAI 881 TCCGGA T^CCGGA
BsiEI 1494 CGGCCG CG[GA][CT]^CG
Bsp1407I 749 TGTACA T^GTACA
BspEI 881 TCCGGA T^CCGGA
BsrBI 1975 GAGCGG CCGCTC(-3/-3)
BsrGI 749 TGTACA T^GTACA
BstAPI 554 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 232 GGATCC [GA]^GATC[CT]
DrdI 566 GACTCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1494 CGGCCG C^GGCCG
EclXI 1494 CGGCCG C^GGCCG
Eco47III 397 AGCGCT AGC^GCT
Eco52I 1494 CGGCCG C^GGCCG
EcoNI 1949 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2373 GATATC GAT^ATC
EcoT14I 1518 CCAAGG C^C[AT][AT]GG
FspI 1629 TGCGCA TGC^GCA
HpaI 2292 GTTAAC GTT^AAC
KasI 1352 GGCGCC G^GCGCC
KspI 1363 CCGCGG CCGC^GG
MflI 232 GGATCC [GA]^GATC[CT]
MroI 881 TCCGGA T^CCGGA
NarI 1352 GGCGCC GG^CGCC
PinAI 2545 ACCGGT A^CCGGT
PmaCI 1733 CACGTG CAC^GTG
PmlI 1733 CACGTG CAC^GTG
SacI 377 GAGCTC GAGCT^C
SacII 1363 CCGCGG CCGC^GG
SfoI 1352 GGCGCC GGC^GCC
SmlI 1577 CTCAAG C^T[CT][GA]AG
SspBI 749 TGTACA T^GTACA
StuI 733 AGGCCT AGG^CCT
StyI 1518 CCAAGG C^C[AT][AT]GG
TaqI 1338 TCGA T^CGA
TthHB8I 1338 TCGA T^CGA
XbaI 3393 TCTAGA T^CTAGA
XcmI 1665 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 232 GGATCC [GA]^GATC[CT]
XmnI 656 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AclI 628 AACGTT AA^CGTT
3508 AACGTT
AspI 467 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2639 GACGGAGTC
BalI 1288 TGGCCA TGG^CCA
2822 TGGCCA
BbvCI 2732 CCTCAGC CCTCAGC(-5/-2)
3592 GCTGAGG
BcgI 2721 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3598 GCAGGAGAATCG
BclI 139 TGATCA T^GATCA
798 TGATCA
BsaWI 881 TCCGGA [AT]^CCGG[AT]
2545 ACCGGT
BsmBI 2637 GAGACG CGTCTC(1/5)
2803 GAGACG
BsrDI 2426 CATTGC GCAATG(2/0)
719 GCAATG
BsrFI 2039 ACCGGC [GA]^CCGG[CT]
2545 ACCGGT
BssSI 3484 CACGAG CACGAG(-5/-1)
2847 CTCGTG
BstEII 1213 GGTCACC G^GT[ACGT]ACC
1513 GGTGACC
BstPI 1213 GGTCACC G^GT[ACGT]ACC
1513 GGTGACC
Cfr10I 2039 ACCGGC [GA]^CCGG[CT]
2545 ACCGGT
DsaI 1363 CCGCGG C^C[GA][CT]GG
1584 CCACGG
EarI 1856 CTCTTC CTCTTC(1/4)
374 GAAGAG
EcoO65I 1213 GGTCACC G^GT[ACGT]ACC
1513 GGTGACC
FbaI 139 TGATCA T^GATCA
798 TGATCA
HgaI 1415 GACGC GACGC(5/10)
1658 GACGC
HincII 947 GTTGAC GT[CT]^[GA]AC
2292 GTTAAC
HindII 947 GTTGAC GT[CT]^[GA]AC
2292 GTTAAC
Ksp632I 1856 CTCTTC CTCTTC(1/4)
374 GAAGAG
MluNI 1288 TGGCCA TGG^CCA
2822 TGGCCA
MscI 1288 TGGCCA TGG^CCA
2822 TGGCCA
PflFI 467 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2639 GACGGAGTC
Psp1406I 628 AACGTT AA^CGTT
3508 AACGTT
PvuII 195 CAGCTG CAG^CTG
1571 CAGCTG
SapI 374 GAAGAGC GCTCTTC(1/4)
1855 GCTCTTC
SbfI 1072 CCTGCAGG CCTGCA^GG
1327 CCTGCAGG
SexAI 1509 ACCTGGT A^CC[AT]GGT
1824 ACCTGGT
Sse8387I 1072 CCTGCAGG CCTGCA^GG
1327 CCTGCAGG
SspI 2357 AATATT AAT^ATT
3142 AATATT
Tth111I 467 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2639 GACGGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmaCI XmaI