##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AviII AvrII BanII BbrPI BbvCI
BcgI BciVI BlnI BsaAI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII BstAPI BstBI BstXI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
FseI FspI HgaI HpaI KpnI
KspI MluI MroI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI Van91I XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccII 88 CGCG CG^CG
AcyI 47 GGCGCC G[GA]^CG[CT]C
AflII 770 CTTAAG C^TTAAG
AflIII 2796 ACATGT A^C[GA][CT]GT
AocI 3475 CCTAAGG CC^T[ACGT]AGG
AspI 1614 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 7 CCCGGG C^[CT]CG[GA]G
BamHI 1743 GGATCC G^GATCC
BbeI 47 GGCGCC GGCGC^C
BbsI 1878 GAAGAC GAAGAC(2/6)
BclI 549 TGATCA T^GATCA
BfrI 770 CTTAAG C^TTAAG
BglI 22 GCCGAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 189 AGATCT A^GATCT
BlpI 319 GCTAAGC GC^T[ACGT]AGC
BmyI 3163 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 319 GCTAAGC GC^T[ACGT]AGC
BpuAI 1878 GAAGAC GAAGAC(2/6)
BsaBI 1214 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 47 GGCGCC G[GA]^CG[CT]C
BsaI 2596 GAGACC GGTCTC(1/5)
BsaWI 2098 ACCGGA [AT]^CCGG[AT]
BsmBI 1762 CGTCTC CGTCTC(1/5)
BsoBI 7 CCCGGG C^[CT]CG[GA]G
Bsp1286I 3163 GTGCCC G[AGT]GC[ACT]^C
BspHI 2545 TCATGA T^CATGA
BspLU11I 2796 ACATGT A^CATGT
BspMI 3258 ACCTGC ACCTGC(4/8)
BsrBI 25 GAGCGG CCGCTC(-3/-3)
BssSI 1582 CTCGTG CACGAG(-5/-1)
Bst1107I 567 GTATAC GTA^TAC
BstEII 1146 GGTTACC G^GT[ACGT]ACC
BstPI 1146 GGTTACC G^GT[ACGT]ACC
BstUI 88 CGCG CG^CG
BstZ17I 567 GTATAC GTA^TAC
Bsu36I 3475 CCTAAGG CC^T[ACGT]AGG
CelII 319 GCTAAGC GC^T[ACGT]AGC
DsaI 2343 CCATGG C^C[GA][CT]GG
Eco81I 3475 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1146 GGTTACC G^GT[ACGT]ACC
EcoRI 221 GAATTC G^AATTC
EcoRV 2431 GATATC GAT^ATC
EcoT22I 3502 ATGCAT ATGCA^T
FbaI 549 TGATCA T^GATCA
Hin1I 47 GGCGCC G[GA]^CG[CT]C
KasI 47 GGCGCC G^GCGCC
MaeII 1761 ACGT A^CGT
MamI 1214 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1338 CATGTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 88 CGCG CG^CG
NarI 47 GGCGCC GG^CGCC
NcoI 2343 CCATGG C^CATGG
NsiI 3502 ATGCAT ATGCA^T
PflFI 1614 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2545 TCATGA T^CATGA
SexAI 2599 ACCTGGT A^CC[AT]GGT
SfoI 47 GGCGCC GGC^GCC
SmaI 7 CCCGGG CCC^GGG
SphI 2173 GCATGC GCATG^C
StuI 73 AGGCCT AGG^CCT
Tth111I 1614 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 7 CCCGGG C^CCGGG
XmaI 7 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 567 GTATAC GT^[AC][GT]AC
1529 GTCTAC
AlwNI 1719 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
2060 CAGTAACTG
BalI 1959 TGGCCA TGG^CCA
2214 TGGCCA
BseRI 2709 GAGGAG GAGGAG(10/8)
3065 GAGGAG
BsmFI 159 GTCCC GGGAC(10/14)
1414 GTCCC
BsrDI 1917 GCAATG GCAATG(2/0)
2278 GCAATG
BstYI 189 AGATCT [GA]^GATC[CT]
1743 GGATCC
DraII 1412 AGGTCCC [GA]G^G[ACGT]CC[CT]
2015 AGGACCT
EcoO109I 1412 AGGTCCC [GA]G^G[ACGT]CC[CT]
2015 AGGACCT
HaeII 3 AGCGCC [GA]GCGC^[CT]
47 GGCGCC
HincII 588 GTCAAC GT[CT]^[GA]AC
2459 GTCAAC
HindII 588 GTCAAC GT[CT]^[GA]AC
2459 GTCAAC
HindIII 715 AAGCTT A^AGCTT
3544 AAGCTT
MfeI 2790 CAATTG C^AATTG
3060 CAATTG
MflI 189 AGATCT [GA]^GATC[CT]
1743 GGATCC
MluNI 1959 TGGCCA TGG^CCA
2214 TGGCCA
MscI 1959 TGGCCA TGG^CCA
2214 TGGCCA
MunI 2790 CAATTG C^AATTG
3060 CAATTG
NdeI 826 CATATG CA^TATG
1953 CATATG
PpuMI 1412 AGGTCCC [GA]G^G[AT]CC[CT]
2015 AGGACCT
PstI 165 CTGCAG CTGCA^G
600 CTGCAG
PvuII 749 CAGCTG CAG^CTG
2451 CAGCTG
XbaI 1866 TCTAGA T^CTAGA
2138 TCTAGA
XhoII 189 AGATCT [GA]^GATC[CT]
1743 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BanI
BbvI BcnI BfaI BpmI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I