##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BfrI BglI BlnI BsaHI
BseAI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstPI
ClaI CpoI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoT22I FauI FseI
FspI HaeII Hin1I KasI KpnI
KspI MluI MroI MspA1I NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmeI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AgeI 749 ACCGGT A^CCGGT
Alw44I 1571 GTGCAC G^TGCAC
AocI 2229 CCTCAGG CC^T[ACGT]AGG
ApaLI 1571 GTGCAC G^TGCAC
Asp700I 418 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 947 GAGCCC G[GA]GC[CT]^C
BbrPI 1852 CACGTG CAC^GTG
BbsI 418 GAAGAC GAAGAC(2/6)
BlpI 1822 GCTCAGC GC^T[ACGT]AGC
BpmI 644 CTGGAG CTGGAG(16/14)
Bpu1102I 1822 GCTCAGC GC^T[ACGT]AGC
BpuAI 418 GAAGAC GAAGAC(2/6)
BsaI 2449 GAGACC GGTCTC(1/5)
BsaWI 749 ACCGGT [AT]^CCGG[AT]
BsmBI 43 GAGACG CGTCTC(1/5)
BspHI 2376 TCATGA T^CATGA
BspLU11I 2424 ACATGT A^CATGT
BsrDI 2331 CATTGC GCAATG(2/0)
BstAPI 2515 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2229 CCTCAGG CC^T[ACGT]AGG
CelII 1822 GCTCAGC GC^T[ACGT]AGC
DraI 1698 TTTAAA TTT^AAA
DraII 740 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 2934 CCATGG C^C[GA][CT]GG
EagI 65 CGGCCG C^GGCCG
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
Eco81I 2229 CCTCAGG CC^T[ACGT]AGG
EcoO109I 740 AGGACCT [GA]G^G[ACGT]CC[CT]
HindIII 1115 AAGCTT A^AGCTT
NaeI 228 GCCGGC GCC^GGC
NcoI 2934 CCATGG C^CATGG
NdeI 1877 CATATG CA^TATG
NgoMIV 228 GCCGGC G^CCGGC
PinAI 749 ACCGGT A^CCGGT
PmaCI 1852 CACGTG CAC^GTG
PmlI 1852 CACGTG CAC^GTG
PpuMI 740 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 2376 TCATGA T^CATGA
SmaI 185 CCCGGG CCC^GGG
XmaCI 185 CCCGGG C^CCGGG
XmaI 185 CCCGGG C^CCGGG
XmnI 418 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1710 GTATAC GT^[AC][GT]AC
1987 GTATAC
AccII 63 CGCG CG^CG
171 CGCG
AflIII 1853 ACGTGT A^C[GA][CT]GT
2424 ACATGT
AlwI 1447 GATCC GGATC(4/5)
1404 GGATC
AlwNI 994 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1279 CAGGCTCTG
AseI 1509 ATTAAT AT^TAAT
2253 ATTAAT
AsnI 1509 ATTAAT AT^TAAT
2253 ATTAAT
AspHI 1571 GTGCAC G[AT]GC[AT]^C
1865 GAGCAC
AvaI 185 CCCGGG C^[CT]CG[GA]G
2153 CCCGAG
BalI 492 TGGCCA TGG^CCA
2393 TGGCCA
BfaI 541 CTAG C^TAG
1828 CTAG
BsaAI 1852 CACGTG [CT]AC^GT[GA]
2823 TACGTG
BsgI 31 GTGCAG GTGCAG(16/14)
1517 GTGCAG
BsiEI 65 CGGCCG CG[GA][CT]^CG
135 CGACCG
BsiHKAI 1571 GTGCAC G[AT]GC[AT]^C
1865 GAGCAC
BsoBI 185 CCCGGG C^[CT]CG[GA]G
2153 CCCGAG
BsrFI 228 GCCGGC [GA]^CCGG[CT]
749 ACCGGT
Bst1107I 1710 GTATAC GTA^TAC
1987 GTATAC
BstUI 63 CGCG CG^CG
171 CGCG
BstXI 1656 CCAGAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2192 CCACGTTCATGG
BstZ17I 1710 GTATAC GTA^TAC
1987 GTATAC
Cfr10I 228 GCCGGC [GA]^CCGG[CT]
749 ACCGGT
EcoNI 957 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2685 CCTGTCCAAGG
EcoRV 1651 GATATC GAT^ATC
2614 GATATC
HgaI 45 GACGC GACGC(5/10)
169 GACGC
HpaI 1048 GTTAAC GTT^AAC
2919 GTTAAC
MaeI 541 CTAG C^TAG
1828 CTAG
MfeI 2176 CAATTG C^AATTG
3038 CAATTG
MluNI 492 TGGCCA TGG^CCA
2393 TGGCCA
MscI 492 TGGCCA TGG^CCA
2393 TGGCCA
MunI 2176 CAATTG C^AATTG
3038 CAATTG
MvnI 63 CGCG CG^CG
171 CGCG
PshBI 1509 ATTAAT AT^TAAT
2253 ATTAAT
PstI 49 CTGCAG CTGCA^G
247 CTGCAG
ScaI 1642 AGTACT AGT^ACT
2079 AGTACT
SphI 98 GCATGC GCATG^C
1938 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BclI BcnI BglII
BmyI BsaBI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII