##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AviII AvrII BalI BanI
BbeI BbrPI BcgI BclI BcnI
BglI BglII BlnI BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HpaII KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NspV PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I StuI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 3067 CTTAAG C^TTAAG
AflIII 2626 ACATGT A^C[GA][CT]GT
AlwNI 3400 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1852 CCTCAGG CC^T[ACGT]AGG
AspHI 2291 GAGCAC G[AT]GC[AT]^C
AspI 2657 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2584 CTCGAG C^[CT]CG[GA]G
BamHI 2718 GGATCC G^GATCC
BanII 1721 GGGCTC G[GA]GC[CT]^C
BbsI 2547 GTCTTC GAAGAC(2/6)
BbvCI 1287 GCTGAGG CCTCAGC(-5/-2)
BfrI 3067 CTTAAG C^TTAAG
BpuAI 2547 GTCTTC GAAGAC(2/6)
BsaBI 834 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2455 CTCCTC GAGGAG(10/8)
BsgI 3217 CTGCAC GTGCAG(16/14)
BsiHKAI 2291 GAGCAC G[AT]GC[AT]^C
BsmI 3355 GCATTC GAATGC(1/-1)
BsoBI 2584 CTCGAG C^[CT]CG[GA]G
BspLU11I 2626 ACATGT A^CATGT
BspMI 3079 ACCTGC ACCTGC(4/8)
BssSI 2294 CACGAG CACGAG(-5/-1)
BstXI 1912 CCAGTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1852 CCTCAGG CC^T[ACGT]AGG
Eco81I 1852 CCTCAGG CC^T[ACGT]AGG
EcoRV 3115 GATATC GAT^ATC
EcoT22I 2930 ATGCAT ATGCA^T
HgaI 1118 GACGC GACGC(5/10)
HindIII 987 AAGCTT A^AGCTT
MamI 834 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 3171 CATATG CA^TATG
NsiI 2930 ATGCAT ATGCA^T
PacI 3186 TTAATTAA TTAAT^TAA
PaeR7I 2584 CTCGAG C^TCGAG
PflFI 2657 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 508 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 3488 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SpeI 2738 ACTAGT A^CTAGT
SphI 1283 GCATGC GCATG^C
Tth111I 2657 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2584 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Asp700I 229 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
352 GAACCTTTTC
BciVI 833 GGATAC GTATCC(6/5)
906 GTATCC
BlpI 1723 GCTCAGC GC^T[ACGT]AGC
2288 GCTGAGC
BmyI 1721 GGGCTC G[AGT]GC[ACT]^C
2291 GAGCAC
BpmI 1297 CTGGAG CTGGAG(16/14)
1879 CTGGAG
Bpu1102I 1723 GCTCAGC GC^T[ACGT]AGC
2288 GCTGAGC
BsaI 1095 GGTCTC GGTCTC(1/5)
2817 GGTCTC
Bsp1286I 1721 GGGCTC G[AGT]GC[ACT]^C
2291 GAGCAC
Bsp1407I 382 TGTACA T^GTACA
1565 TGTACA
BsrGI 382 TGTACA T^GTACA
1565 TGTACA
CelII 1723 GCTCAGC GC^T[ACGT]AGC
2288 GCTGAGC
DraII 508 GGGACCT [GA]G^G[ACGT]CC[CT]
1452 GGGGCCT
EcoO109I 508 GGGACCT [GA]G^G[ACGT]CC[CT]
1452 GGGGCCT
EcoT14I 2034 CCAAGG C^C[AT][AT]GG
2414 CCTTGG
FauI 1389 GCGGG CCCGC(4/6)
1728 GCGGG
HincII 331 GTTAAC GT[CT]^[GA]AC
919 GTTAAC
HindII 331 GTTAAC GT[CT]^[GA]AC
919 GTTAAC
HpaI 331 GTTAAC GTT^AAC
919 GTTAAC
NheI 1121 GCTAGC G^CTAGC
2028 GCTAGC
PflMI 615 CCATTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCATCTTTTGG
PleI 1732 GACTC GAGTC(4/5)
2680 GACTC
ScaI 580 AGTACT AGT^ACT
1633 AGTACT
SmlI 2584 CTCGAG C^T[CT][GA]AG
3067 CTTAAG
SspBI 382 TGTACA T^GTACA
1565 TGTACA
StyI 2034 CCAAGG C^C[AT][AT]GG
2414 CCTTGG
SwaI 1006 ATTTAAAT ATTT^AAAT
3131 ATTTAAAT
Van91I 615 CCATTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCATCTTTTGG
XbaI 342 TCTAGA T^CTAGA
473 TCTAGA
XmnI 229 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
352 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BbvI
BfaI BsaJI BsiYI BslI BsmAI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII