##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AclI AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AviII AvrII
BbeI BbrPI BbsI BciVI BlnI
BpuAI BsaAI BsaBI BsiEI BsiWI
Bsp1407I BspDI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoRV FseI FspI HgaI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 591 GACGTC GACGT^C
AccII 2894 CGCG CG^CG
AcyI 591 GACGTC G[GA]^CG[CT]C
AhdI 586 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1086 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 103 GGGCCC GGGCC^C
AseI 2552 ATTAAT AT^TAAT
AsnI 2552 ATTAAT AT^TAAT
Asp700I 764 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 586 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3347 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1157 GGATCC G^GATCC
BanI 2755 GGTGCC G^G[CT][GA]CC
BcgI 129 CGAGCACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 52 CCGGG CC^[GC]GG
BglI 1810 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 526 AGATCT A^GATCT
BlpI 36 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 36 GCTCAGC GC^T[ACGT]AGC
BsaHI 591 GACGTC G[GA]^CG[CT]C
BseRI 1925 GAGGAG GAGGAG(10/8)
BsmBI 593 CGTCTC CGTCTC(1/5)
BsmI 1231 GAATGC GAATGC(1/-1)
BspMI 3037 GCAGGT ACCTGC(4/8)
BssSI 3259 CACGAG CACGAG(-5/-1)
BstUI 2894 CGCG CG^CG
CelII 36 GCTCAGC GC^T[ACGT]AGC
Eam1105I 586 GACACGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 591 GACGTC G[GA]^CG[CT]C
MvnI 2894 CGCG CG^CG
NciI 52 CCGGG CC^[GC]GG
NdeI 2127 CATATG CA^TATG
PaeR7I 1548 CTCGAG C^TCGAG
PflFI 3347 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 300 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1830 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2552 ATTAAT AT^TAAT
PstI 921 CTGCAG CTGCA^G
SacI 504 GAGCTC GAGCT^C
SapI 506 GCTCTTC GCTCTTC(1/4)
ScaI 534 AGTACT AGT^ACT
SphI 3223 GCATGC GCATG^C
SspI 2672 AATATT AAT^ATT
SwaI 3188 ATTTAAAT ATTT^AAAT
Tth111I 3347 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1641 CCATGAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1548 CTCGAG C^TCGAG
XmnI 764 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 601 GTCTAC GT^[AC][GT]AC
2328 GTCTAC
AccIII 897 TCCGGA T^CCGGA
1568 TCCGGA
AflII 295 CTTAAG C^TTAAG
2631 CTTAAG
AflIII 961 ACATGT A^C[GA][CT]GT
1831 ACATGT
AocI 811 CCTAAGG CC^T[ACGT]AGG
2951 CCTGAGG
AspHI 130 GAGCAC G[AT]GC[AT]^C
504 GAGCTC
AvaII 301 GGACC G^G[AT]CC
1079 GGACC
BclI 337 TGATCA T^GATCA
1519 TGATCA
BfaI 1872 CTAG C^TAG
2580 CTAG
BfrI 295 CTTAAG C^TTAAG
2631 CTTAAG
BsaI 2830 GAGACC GGTCTC(1/5)
2968 GAGACC
BsaWI 897 TCCGGA [AT]^CCGG[AT]
1568 TCCGGA
BseAI 897 TCCGGA T^CCGGA
1568 TCCGGA
BsiHKAI 130 GAGCAC G[AT]GC[AT]^C
504 GAGCTC
BspEI 897 TCCGGA T^CCGGA
1568 TCCGGA
BspHI 705 TCATGA T^CATGA
1738 TCATGA
BspLU11I 961 ACATGT A^CATGT
1831 ACATGT
BsrDI 2318 CATTGC GCAATG(2/0)
1781 GCAATG
BstEII 1636 GGTTACC G^GT[ACGT]ACC
2008 GGTTACC
BstPI 1636 GGTTACC G^GT[ACGT]ACC
2008 GGTTACC
Bsu36I 811 CCTAAGG CC^T[ACGT]AGG
2951 CCTGAGG
Eco81I 811 CCTAAGG CC^T[ACGT]AGG
2951 CCTGAGG
EcoO65I 1636 GGTTACC G^GT[ACGT]ACC
2008 GGTTACC
EcoRI 1010 GAATTC G^AATTC
1065 GAATTC
EcoT22I 741 ATGCAT ATGCA^T
1298 ATGCAT
FbaI 337 TGATCA T^GATCA
1519 TGATCA
HaeII 40 AGCGCC [GA]GCGC^[CT]
637 AGCGCC
HindIII 2033 AAGCTT A^AGCTT
2187 AAGCTT
MaeI 1872 CTAG C^TAG
2580 CTAG
MroI 897 TCCGGA T^CCGGA
1568 TCCGGA
NcoI 2461 CCATGG C^CATGG
2759 CCATGG
NsiI 741 ATGCAT ATGCA^T
1298 ATGCAT
RcaI 705 TCATGA T^CATGA
1738 TCATGA
SfcI 921 CTGCAG C^T[GA][CT]AG
2050 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI BalI BanII BbvCI
BbvI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII