##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII BamHI
BbeI BbrPI BbvCI BfrI BlpI
Bpu1102I BsaAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FseI
FspI HaeII HpaI KasI KpnI
MluI NarI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI SspI SwaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2029 TCCGGA T^CCGGA
AclI 441 AACGTT AA^CGTT
AcyI 1657 GGCGTC G[GA]^CG[CT]C
AlwNI 3586 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 2022 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2159 CCTAGG C^CTAGG
BcgI 983 GCACCAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2715 GGATAC GTATCC(6/5)
BclI 797 TGATCA T^GATCA
BglI 300 GCCTTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2159 CCTAGG C^CTAGG
BsaHI 1657 GGCGTC G[GA]^CG[CT]C
BsaWI 2029 TCCGGA [AT]^CCGG[AT]
BseAI 2029 TCCGGA T^CCGGA
BspEI 2029 TCCGGA T^CCGGA
BspLU11I 3561 ACATGT A^CATGT
BspMI 169 GCAGGT ACCTGC(4/8)
BsrFI 2794 GCCGGC [GA]^CCGG[CT]
BssSI 1915 CACGAG CACGAG(-5/-1)
BstEII 2019 GGTGACC G^GT[ACGT]ACC
BstPI 2019 GGTGACC G^GT[ACGT]ACC
BstXI 2705 CCATGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2794 GCCGGC [GA]^CCGG[CT]
EcoO65I 2019 GGTGACC G^GT[ACGT]ACC
EcoRI 2399 GAATTC G^AATTC
FbaI 797 TGATCA T^GATCA
HgaI 1658 GCGTC GACGC(5/10)
Hin1I 1657 GGCGTC G[GA]^CG[CT]C
KspI 134 CCGCGG CCGC^GG
MroI 2029 TCCGGA T^CCGGA
NaeI 2794 GCCGGC GCC^GGC
NgoMIV 2794 GCCGGC G^CCGGC
NheI 1727 GCTAGC G^CTAGC
NspI 3561 ACATGT [GA]CATG^[CT]
PflFI 2022 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 441 AACGTT AA^CGTT
SacII 134 CCGCGG CCGC^GG
SalI 1342 GTCGAC G^TCGAC
SpeI 2382 ACTAGT A^CTAGT
Tth111I 2022 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 728 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 1342 GTCGAC GT^[AC][GT]AC
3475 GTAGAC
AflIII 2054 ACACGT A^C[GA][CT]GT
3561 ACATGT
BanI 183 GGCACC G^G[CT][GA]CC
2508 GGTGCC
BglII 887 AGATCT A^GATCT
2075 AGATCT
BpmI 3658 CTCCAG CTGGAG(16/14)
3126 CTGGAG
BsaI 3700 GAGACC GGTCTC(1/5)
914 GGTCTC
BsrBI 1960 GAGCGG CCGCTC(-3/-3)
2172 GAGCGG
BsrDI 2082 GCAATG GCAATG(2/0)
2280 GCAATG
DraI 3685 TTTAAA TTT^AAA
3717 TTTAAA
HincII 1342 GTCGAC GT[CT]^[GA]AC
2547 GTCAAC
HindII 1342 GTCGAC GT[CT]^[GA]AC
2547 GTCAAC
MfeI 754 CAATTG C^AATTG
1168 CAATTG
MunI 754 CAATTG C^AATTG
1168 CAATTG
NcoI 1772 CCATGG C^CATGG
2705 CCATGG
PpuMI 3101 AGGACCC [GA]G^G[AT]CC[CT]
3528 AGGTCCC
PstI 155 CTGCAG CTGCA^G
1099 CTGCAG
PvuII 146 CAGCTG CAG^CTG
2944 CAGCTG
SexAI 1154 ACCAGGT A^CC[AT]GGT
2023 ACCTGGT
StuI 2156 AGGCCT AGG^CCT
2236 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaBI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII