##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI AviII
AvrII BalI BamHI BbrPI BbsI
BcgI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspMI BsrBI BsrFI BssHII
BstAPI CelII Cfr10I ClaI CpoI
DraII EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoO109I FseI FspI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI NaeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SgrAI SnaBI
SphI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
Acc65I 3332 GGTACC G^GTACC
AccII 3561 CGCG CG^CG
AcyI 3477 GGCGCC G[GA]^CG[CT]C
AflII 907 CTTAAG C^TTAAG
AflIII 2456 ACATGT A^C[GA][CT]GT
AhdI 2083 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2541 CCTGAGG CC^T[ACGT]AGG
Asp718I 3332 GGTACC G^GTACC
AspEI 2083 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 230 GTGCTC G[AT]GC[AT]^C
AspI 3596 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 2082 GGACC G^G[AT]CC
BanII 1740 GGGCTC G[GA]GC[CT]^C
BbeI 3477 GGCGCC GGCGC^C
BciVI 3063 GGATAC GTATCC(6/5)
BclI 1454 TGATCA T^GATCA
BfrI 907 CTTAAG C^TTAAG
BglII 1755 AGATCT A^GATCT
BpmI 2738 CTCCAG CTGGAG(16/14)
BsaHI 3477 GGCGCC G[GA]^CG[CT]C
BsaI 3417 GAGACC GGTCTC(1/5)
BsaWI 55 TCCGGT [AT]^CCGG[AT]
BsiHKAI 230 GTGCTC G[AT]GC[AT]^C
Bsp1407I 1416 TGTACA T^GTACA
BspLU11I 2456 ACATGT A^CATGT
BsrDI 64 GCAATG GCAATG(2/0)
BsrGI 1416 TGTACA T^GTACA
Bst1107I 1547 GTATAC GTA^TAC
BstBI 254 TTCGAA TT^CGAA
BstUI 3561 CGCG CG^CG
BstZ17I 1547 GTATAC GTA^TAC
Bsu36I 2541 CCTGAGG CC^T[ACGT]AGG
Cfr13I 2082 GGACC G^G[ACGT]CC
DraIII 2352 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 70 GACGGAATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3421 CCATGG C^C[GA][CT]GG
Eam1105I 2083 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2541 CCTGAGG CC^T[ACGT]AGG
EcoRI 671 GAATTC G^AATTC
EcoRV 1078 GATATC GAT^ATC
EcoT14I 3421 CCATGG C^C[AT][AT]GG
EcoT22I 2121 ATGCAT ATGCA^T
FbaI 1454 TGATCA T^GATCA
HaeII 3477 GGCGCC [GA]GCGC^[CT]
HgaI 2727 GCGTC GACGC(5/10)
Hin1I 3477 GGCGCC G[GA]^CG[CT]C
HpaI 1470 GTTAAC GTT^AAC
KasI 3477 GGCGCC G^GCGCC
KpnI 3332 GGTACC GGTAC^C
MaeII 3090 ACGT A^CGT
MvnI 3561 CGCG CG^CG
NarI 3477 GGCGCC GG^CGCC
NcoI 3421 CCATGG C^CATGG
NdeI 2293 CATATG CA^TATG
NsiI 2121 ATGCAT ATGCA^T
NspI 2456 ACATGT [GA]CATG^[CT]
NspV 254 TTCGAA TT^CGAA
PflFI 3596 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1668 CTGCAG CTGCA^G
Sau96I 2082 GGACC G^G[ACGT]CC
SfoI 3477 GGCGCC GGC^GCC
SfuI 254 TTCGAA TT^CGAA
SmaI 2694 CCCGGG CCC^GGG
SspBI 1416 TGTACA T^GTACA
StyI 3421 CCATGG C^C[AT][AT]GG
Tth111I 3596 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2694 CCCGGG C^CCGGG
XmaI 2694 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1547 GTATAC GT^[AC][GT]AC
2591 GTCTAC
AlwNI 278 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
2234 CAGCCTCTG
Asp700I 999 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2161 GAACCTATTC
AvaI 2694 CCCGGG C^[CT]CG[GA]G
3497 CTCGGG
BseRI 2221 CTCCTC GAGGAG(10/8)
2824 GAGGAG
BsoBI 2694 CCCGGG C^[CT]CG[GA]G
3497 CTCGGG
BssSI 3400 CACGAG CACGAG(-5/-1)
239 CTCGTG
BstEII 318 GGTGACC G^GT[ACGT]ACC
1961 GGTGACC
BstPI 318 GGTGACC G^GT[ACGT]ACC
1961 GGTGACC
CfoI 3478 GCGC GCG^C
3562 GCGC
EcoO65I 318 GGTGACC G^GT[ACGT]ACC
1961 GGTGACC
FauI 2562 CCCGC CCCGC(4/6)
2529 GCGGG
HhaI 3478 GCGC GCG^C
3562 GCGC
HinP1I 3478 GCGC G^CGC
3562 GCGC
HindIII 47 AAGCTT A^AGCTT
1201 AAGCTT
PflMI 2065 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2233 CCAGCCTCTGG
SapI 3242 GAAGAGC GCTCTTC(1/4)
1342 GCTCTTC
SfaNI 2724 GATGC GCATC(5/9)
1719 GCATC
SfcI 1668 CTGCAG C^T[GA][CT]AG
3482 CTGTAG
SmlI 907 CTTAAG C^T[CT][GA]AG
1873 CTCAAG
StuI 1780 AGGCCT AGG^CCT
2801 AGGCCT
Van91I 2065 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2233 CCAGCCTCTGG
XcmI 1803 CCAGCTTCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3336 CCAGGCTGGGCGTGG
XmnI 999 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2161 GAACCTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI BanI BbvCI
BbvI BcnI BfaI BmyI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PshBI RsaI Sau3AI ScaI ScrFI
SpeI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII