##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AccII AccIII AclI AflII AflIII
AgeI AhdI Aor51HI ApaI AscI
AspEI AspI AviII AvrII BbeI
BbrPI BfrI BglI BlnI BsaAI
BsaBI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HhaI
HinP1I KasI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1491 GACGTC GACGT^C
Acc65I 25 GGTACC G^GTACC
AlwNI 187 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 251 ATTAAT AT^TAAT
AsnI 251 ATTAAT AT^TAAT
Asp700I 2369 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 25 GGTACC G^GTACC
AvaI 875 CCCGAG C^[CT]CG[GA]G
BalI 1519 TGGCCA TGG^CCA
BbsI 2325 GTCTTC GAAGAC(2/6)
BcgI 1656 CGAAGGATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1192 GGATAC GTATCC(6/5)
BcnI 2927 CCGGG CC^[GC]GG
BglII 1291 AGATCT A^GATCT
BpuAI 2325 GTCTTC GAAGAC(2/6)
BsaI 1847 GGTCTC GGTCTC(1/5)
BsaWI 158 ACCGGA [AT]^CCGG[AT]
BsgI 450 CTGCAC GTGCAG(16/14)
BsiEI 156 CGACCG CG[GA][CT]^CG
BsmAI 1848 GTCTC GTCTC(1/5)
BsoBI 875 CCCGAG C^[CT]CG[GA]G
BspHI 2755 TCATGA T^CATGA
BsrDI 898 CATTGC GCAATG(2/0)
BssSI 2238 CTCGTG CACGAG(-5/-1)
EaeI 1519 TGGCCA [CT]^GGCC[GA]
FauI 22 GCGGG CCCGC(4/6)
HgaI 786 GACGC GACGC(5/10)
HpaI 2362 GTTAAC GTT^AAC
KpnI 25 GGTACC GGTAC^C
MluNI 1519 TGGCCA TGG^CCA
MscI 1519 TGGCCA TGG^CCA
NciI 2927 CCGGG CC^[GC]GG
PshAI 2174 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 251 ATTAAT AT^TAAT
RcaI 2755 TCATGA T^CATGA
SapI 665 GAAGAGC GCTCTTC(1/4)
ScaI 1122 AGTACT AGT^ACT
SexAI 2969 ACCTGGT A^CC[AT]GGT
SphI 3254 GCATGC GCATG^C
StuI 1718 AGGCCT AGG^CCT
XmnI 2369 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3200 GTCTAC GT^[AC][GT]AC
3705 GTCTAC
AcyI 786 GACGCC G[GA]^CG[CT]C
1491 GACGTC
AocI 165 CCTGAGG CC^T[ACGT]AGG
1714 CCTGAGG
BamHI 161 GGATCC G^GATCC
2524 GGATCC
BanI 25 GGTACC G^G[CT][GA]CC
2297 GGCACC
BbvCI 371 CCTCAGC CCTCAGC(-5/-2)
1838 CCTCAGC
BlpI 692 GCTCAGC GC^T[ACGT]AGC
1597 GCTAAGC
Bpu1102I 692 GCTCAGC GC^T[ACGT]AGC
1597 GCTAAGC
BsaHI 786 GACGCC G[GA]^CG[CT]C
1491 GACGTC
Bsu36I 165 CCTGAGG CC^T[ACGT]AGG
1714 CCTGAGG
CelII 692 GCTCAGC GC^T[ACGT]AGC
1597 GCTAAGC
DraI 3127 TTTAAA TTT^AAA
3568 TTTAAA
DsaI 1485 CCATGG C^C[GA][CT]GG
1522 CCATGG
Eco81I 165 CCTGAGG CC^T[ACGT]AGG
1714 CCTGAGG
EcoNI 871 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1183 CCTGCTGCAGG
EcoT14I 1485 CCATGG C^C[AT][AT]GG
1522 CCATGG
HapII 159 CCGG C^CGG
2927 CCGG
Hin1I 786 GACGCC G[GA]^CG[CT]C
1491 GACGTC
HpaII 159 CCGG C^CGG
2927 CCGG
MspI 159 CCGG C^CGG
2927 CCGG
NcoI 1485 CCATGG C^CATGG
1522 CCATGG
PstI 40 CTGCAG CTGCA^G
1187 CTGCAG
SacI 3 GAGCTC GAGCT^C
590 GAGCTC
StyI 1485 CCATGG C^C[AT][AT]GG
1522 CCATGG
TaqI 1655 TCGA T^CGA
2353 TCGA
TthHB8I 1655 TCGA T^CGA
2353 TCGA
XcmI 1104 CCAAGTCCTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1371 CCAGGATGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaLI ApoI AspHI AvaII
BanII BbvI BclI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII FbaI Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII