##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI BbrPI BbsI
BbvCI BfrI BpuAI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eco47III EcoRI EcoRV EcoT22I FseI
HpaI KpnI KspI MfeI MluI
MroI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1320 GACGTC GACGT^C
AhdI 1097 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1097 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1237 TGCGCA TGC^GCA
AvrII 2344 CCTAGG C^CTAGG
BamHI 2462 GGATCC G^GATCC
BcgI 2024 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 184 GTATCC GTATCC(6/5)
BglII 1846 AGATCT A^GATCT
BlnI 2344 CCTAGG C^CTAGG
BsaAI 1731 CACGTA [CT]AC^GT[GA]
BsaBI 2168 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3061 GAGACC GGTCTC(1/5)
BsiEI 1960 CGGCCG CG[GA][CT]^CG
BsmBI 1539 CGTCTC CGTCTC(1/5)
BsrBI 2645 CCGCTC CCGCTC(-3/-3)
BsrFI 48 GCCGGC [GA]^CCGG[CT]
BssSI 1053 CTCGTG CACGAG(-5/-1)
BstEII 584 GGTGACC G^GT[ACGT]ACC
BstPI 584 GGTGACC G^GT[ACGT]ACC
Cfr10I 48 GCCGGC [GA]^CCGG[CT]
DrdI 1320 GACGTCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1960 CGGCCG C^GGCCG
Eam1105I 1097 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1960 CGGCCG C^GGCCG
Eco52I 1960 CGGCCG C^GGCCG
EcoNI 872 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 584 GGTGACC G^GT[ACGT]ACC
FspI 1237 TGCGCA TGC^GCA
HincII 1323 GTCAAC GT[CT]^[GA]AC
HindII 1323 GTCAAC GT[CT]^[GA]AC
HindIII 2880 AAGCTT A^AGCTT
MamI 2168 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 48 GCCGGC GCC^GGC
NgoMIV 48 GCCGGC G^CCGGC
PshAI 2630 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 1822 CCCGGG CCC^GGG
SphI 808 GCATGC GCATG^C
XbaI 2396 TCTAGA T^CTAGA
XcmI 1831 CCATGAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1822 CCCGGG C^CCGGG
XmaI 1822 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 2463 GATCC GGATC(4/5)
2462 GGATC
AocI 1509 CCTGAGG CC^T[ACGT]AGG
2306 CCTCAGG
BclI 1021 TGATCA T^GATCA
1621 TGATCA
BlpI 812 GCTCAGC GC^T[ACGT]AGC
2474 GCTGAGC
Bpu1102I 812 GCTCAGC GC^T[ACGT]AGC
2474 GCTGAGC
BsaWI 1800 ACCGGA [AT]^CCGG[AT]
2089 ACCGGA
BstYI 1846 AGATCT [GA]^GATC[CT]
2462 GGATCC
Bsu36I 1509 CCTGAGG CC^T[ACGT]AGG
2306 CCTCAGG
CelII 812 GCTCAGC GC^T[ACGT]AGC
2474 GCTGAGC
DsaI 1342 CCACGG C^C[GA][CT]GG
2104 CCGTGG
EarI 2124 CTCTTC CTCTTC(1/4)
3327 CTCTTC
Eco81I 1509 CCTGAGG CC^T[ACGT]AGG
2306 CCTCAGG
FbaI 1021 TGATCA T^GATCA
1621 TGATCA
Ksp632I 2124 CTCTTC CTCTTC(1/4)
3327 CTCTTC
MflI 1846 AGATCT [GA]^GATC[CT]
2462 GGATCC
StuI 250 AGGCCT AGG^CCT
2540 AGGCCT
XhoII 1846 AGATCT [GA]^GATC[CT]
2462 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmlI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I