##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
ApoI AseI AsnI Asp700I Asp718I
BalI BamHI BcgI BciVI BclI
BfrI BlpI Bpu1102I BsiWI BspDI
BspHI BssSI Bst1107I BstBI BstZ17I
CelII ClaI DraI EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MfeI MluI
MluNI MscI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tru9I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 652 GTCTAC GT^[AC][GT]AC
AccIII 476 TCCGGA T^CCGGA
Alw44I 2984 GTGCAC G^TGCAC
AocI 771 CCTGAGG CC^T[ACGT]AGG
ApaLI 2984 GTGCAC G^TGCAC
AscI 103 GGCGCGCC GG^CGCGCC
AspI 646 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1655 TGCGCA TGC^GCA
AvrII 345 CCTAGG C^CTAGG
BbvCI 537 CCTCAGC CCTCAGC(-5/-2)
BglII 848 AGATCT A^GATCT
BlnI 345 CCTAGG C^CTAGG
BsaBI 576 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 476 TCCGGA [AT]^CCGG[AT]
BseAI 476 TCCGGA T^CCGGA
BsiEI 2007 CGGCCG CG[GA][CT]^CG
BsmBI 328 GAGACG CGTCTC(1/5)
BsmI 2369 GAATGC GAATGC(1/-1)
BspEI 476 TCCGGA T^CCGGA
BspLU11I 596 ACATGT A^CATGT
BsrBI 91 GAGCGG CCGCTC(-3/-3)
BsrDI 1523 GCAATG GCAATG(2/0)
BssHII 104 GCGCGC G^CGCGC
BstAPI 1035 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 79 GGTGACC G^GT[ACGT]ACC
BstPI 79 GGTGACC G^GT[ACGT]ACC
BstXI 1793 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 771 CCTGAGG CC^T[ACGT]AGG
CpoI 94 CGGACCG CG^G[AT]CCG
EagI 2007 CGGCCG C^GGCCG
EclXI 2007 CGGCCG C^GGCCG
Eco52I 2007 CGGCCG C^GGCCG
Eco81I 771 CCTGAGG CC^T[ACGT]AGG
EcoO65I 79 GGTGACC G^GT[ACGT]ACC
FspI 1655 TGCGCA TGC^GCA
KspI 1107 CCGCGG CCGC^GG
MamI 576 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 476 TCCGGA T^CCGGA
NaeI 56 GCCGGC GCC^GGC
NcoI 1799 CCATGG C^CATGG
NgoMIV 56 GCCGGC G^CCGGC
PflFI 646 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1051 CTGCAG CTGCA^G
RsrII 94 CGGACCG CG^G[AT]CCG
SacI 415 GAGCTC GAGCT^C
SacII 1107 CCGCGG CCGC^GG
SexAI 1505 ACCTGGT A^CC[AT]GGT
SmaI 2001 CCCGGG CCC^GGG
SphI 155 GCATGC GCATG^C
StuI 2574 AGGCCT AGG^CCT
TfiI 1168 GAATC G^A[AT]TC
Tth111I 646 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2001 CCCGGG C^CCGGG
XmaI 2001 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 909 GACGTC GACGT^C
2383 GACGTC
AhdI 480 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
833 GACTCAAGGTC
AlwI 1059 GATCC GGATC(4/5)
2486 GGATC
Aor51HI 293 AGCGCT AGC^GCT
2978 AGCGCT
AspEI 480 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
833 GACTCAAGGTC
BbeI 451 GGCGCC GGCGC^C
1259 GGCGCC
BbrPI 1296 CACGTG CAC^GTG
2989 CACGTG
BglI 890 GCCTCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1668 GCCCGACTGGC
BsaI 487 GGTCTC GGTCTC(1/5)
1509 GGTCTC
BsgI 754 CTGCAC GTGCAG(16/14)
1438 GTGCAG
Bsp1407I 611 TGTACA T^GTACA
2880 TGTACA
BsrFI 56 GCCGGC [GA]^CCGG[CT]
1283 ACCGGC
BsrGI 611 TGTACA T^GTACA
2880 TGTACA
BstYI 848 AGATCT [GA]^GATC[CT]
1058 AGATCC
Cfr10I 56 GCCGGC [GA]^CCGG[CT]
1283 ACCGGC
DraIII 1180 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1432 CACACGGTG
DrdI 909 GACGTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2747 GACAGCACAGTC
Eam1105I 480 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
833 GACTCAAGGTC
Eco47III 293 AGCGCT AGC^GCT
2978 AGCGCT
EcoNI 815 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2836 CCTCAAGCAGG
EcoT14I 345 CCTAGG C^C[AT][AT]GG
1799 CCATGG
KasI 451 GGCGCC G^GCGCC
1259 GGCGCC
MflI 848 AGATCT [GA]^GATC[CT]
1058 AGATCC
NarI 451 GGCGCC GG^CGCC
1259 GGCGCC
PmaCI 1296 CACGTG CAC^GTG
2989 CACGTG
PmlI 1296 CACGTG CAC^GTG
2989 CACGTG
SfcI 1051 CTGCAG C^T[GA][CT]AG
2356 CTATAG
SfoI 451 GGCGCC GGC^GCC
1259 GGCGCC
SspBI 611 TGTACA T^GTACA
2880 TGTACA
StyI 345 CCTAGG C^C[AT][AT]GG
1799 CCATGG
XhoII 848 AGATCT [GA]^GATC[CT]
1058 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI