##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BbrPI BbsI
BbvCI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsiWI BsmBI BsoBI BspHI
BsrDI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DraI DsaI Eam1105I Eco81I EcoO65I
EcoT14I FseI FspI HgaI KpnI
KspI MamI MluI MslI NaeI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SmlI SnaBI SphI Sse8387I StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 2005 TCCGGA T^CCGGA
AgeI 1284 ACCGGT A^CCGGT
BalI 2074 TGGCCA TGG^CCA
BamHI 2548 GGATCC G^GATCC
BclI 936 TGATCA T^GATCA
BseAI 2005 TCCGGA T^CCGGA
BsiEI 426 CGGCCG CG[GA][CT]^CG
BsmI 1623 GAATGC GAATGC(1/-1)
BspDI 1958 ATCGAT AT^CGAT
BspEI 2005 TCCGGA T^CCGGA
BspMI 1042 GCAGGT ACCTGC(4/8)
BstBI 2292 TTCGAA TT^CGAA
BstXI 957 CCAAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1958 ATCGAT AT^CGAT
DrdI 728 GACCACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 426 CGGCCG C^GGCCG
EclXI 426 CGGCCG C^GGCCG
Eco52I 426 CGGCCG C^GGCCG
EcoNI 1132 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1749 GATATC GAT^ATC
FbaI 936 TGATCA T^GATCA
HindIII 915 AAGCTT A^AGCTT
MfeI 1196 CAATTG C^AATTG
MluNI 2074 TGGCCA TGG^CCA
MroI 2005 TCCGGA T^CCGGA
MscI 2074 TGGCCA TGG^CCA
MunI 1196 CAATTG C^AATTG
NdeI 1547 CATATG CA^TATG
NspV 2292 TTCGAA TT^CGAA
PflMI 1472 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1284 ACCGGT A^CCGGT
PpuMI 2146 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 821 CAGCTG CAG^CTG
SapI 2226 GCTCTTC GCTCTTC(1/4)
ScaI 2024 AGTACT AGT^ACT
SfuI 2292 TTCGAA TT^CGAA
SspI 2042 AATATT AAT^ATT
Van91I 1472 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2714 CCAAGTGGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 208 GGCGCC G[GA]^CG[CT]C
331 GGCGCC
Aor51HI 369 AGCGCT AGC^GCT
390 AGCGCT
Asp700I 914 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3125 GAAGAACTTC
BbeI 208 GGCGCC GGCGC^C
331 GGCGCC
BcgI 170 GCAGCGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1294 GCACCTCCTTCG
BsaHI 208 GGCGCC G[GA]^CG[CT]C
331 GGCGCC
BsaI 91 GAGACC GGTCTC(1/5)
431 GAGACC
BsmFI 446 GTCCC GGGAC(10/14)
2267 GTCCC
BspLU11I 842 ACATGT A^CATGT
857 ACATGT
BsrBI 33 GAGCGG CCGCTC(-3/-3)
214 GAGCGG
BsrFI 463 GCCGGT [GA]^CCGG[CT]
1284 ACCGGT
BstYI 1002 AGATCC [GA]^GATC[CT]
2548 GGATCC
Cfr10I 463 GCCGGT [GA]^CCGG[CT]
1284 ACCGGT
DraIII 1473 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2187 CACCTAGTG
EaeI 426 CGGCCG [CT]^GGCC[GA]
2074 TGGCCA
Eco47III 369 AGCGCT AGC^GCT
390 AGCGCT
EcoT22I 1211 ATGCAT ATGCA^T
1317 ATGCAT
Hin1I 208 GGCGCC G[GA]^CG[CT]C
331 GGCGCC
HpaI 2830 GTTAAC GTT^AAC
3446 GTTAAC
KasI 208 GGCGCC G^GCGCC
331 GGCGCC
MflI 1002 AGATCC [GA]^GATC[CT]
2548 GGATCC
NarI 208 GGCGCC GG^CGCC
331 GGCGCC
NsiI 1211 ATGCAT ATGCA^T
1317 ATGCAT
SfcI 1799 CTACAG C^T[GA][CT]AG
2838 CTATAG
SfoI 208 GGCGCC GGC^GCC
331 GGCGCC
SpeI 1913 ACTAGT A^CTAGT
2162 ACTAGT
XhoII 1002 AGATCC [GA]^GATC[CT]
2548 GGATCC
XmnI 914 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3125 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SspBI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I