##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AccIII AgeI AhdI Alw44I ApaLI
AscI AspEI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BlnI
BsaAI BsaBI BseAI BsiWI BsmBI
BspEI BsrBI BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CpoI DrdI Eam1105I EcoO65I EcoRI
EcoRV FseI FspI HgaI HpaI
KasI MamI MluI MroI NarI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PvuI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1946 GACGTC GACGT^C
Acc65I 115 GGTACC G^GTACC
AccI 1777 GTAGAC GT^[AC][GT]AC
AclI 327 AACGTT AA^CGTT
AcyI 1946 GACGTC G[GA]^CG[CT]C
AflII 2539 CTTAAG C^TTAAG
AflIII 3650 ACATGT A^C[GA][CT]GT
AseI 2902 ATTAAT AT^TAAT
AsnI 2902 ATTAAT AT^TAAT
Asp718I 115 GGTACC G^GTACC
AspI 2065 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2158 GTATCC GTATCC(6/5)
BfrI 2539 CTTAAG C^TTAAG
BglI 816 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1946 GACGTC G[GA]^CG[CT]C
BsaWI 3438 ACCGGA [AT]^CCGG[AT]
BsmI 3416 GCATTC GAATGC(1/-1)
Bsp1407I 3636 TGTACA T^GTACA
BspDI 2506 ATCGAT AT^CGAT
BspHI 2874 TCATGA T^CATGA
BspLU11I 3650 ACATGT A^CATGT
BspMI 3452 ACCTGC ACCTGC(4/8)
BsrGI 3636 TGTACA T^GTACA
BssHII 15 GCGCGC G^CGCGC
ClaI 2506 ATCGAT AT^CGAT
DraI 3405 TTTAAA TTT^AAA
DraIII 515 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 237 CGGCCG C^GGCCG
EclXI 237 CGGCCG C^GGCCG
Eco52I 237 CGGCCG C^GGCCG
EcoNI 813 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 998 ATGCAT ATGCA^T
Hin1I 1946 GACGTC G[GA]^CG[CT]C
KpnI 115 GGTACC GGTAC^C
KspI 103 CCGCGG CCGC^GG
MfeI 3356 CAATTG C^AATTG
MslI 529 CACTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3356 CAATTG C^AATTG
NaeI 239 GCCGGC GCC^GGC
NcoI 2371 CCATGG C^CATGG
NgoMIV 239 GCCGGC G^CCGGC
NheI 175 GCTAGC G^CTAGC
NsiI 998 ATGCAT ATGCA^T
PflFI 2065 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2234 CCATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 121 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1487 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2902 ATTAAT AT^TAAT
Psp1406I 327 AACGTT AA^CGTT
RcaI 2874 TCATGA T^CATGA
SacII 103 CCGCGG CCGC^GG
ScaI 3431 AGTACT AGT^ACT
SmaI 111 CCCGGG CCC^GGG
SspBI 3636 TGTACA T^GTACA
Tth111I 2065 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2234 CCATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1474 TCTAGA T^CTAGA
XmaCI 111 CCCGGG C^CCGGG
XmaI 111 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Aor51HI 2738 AGCGCT AGC^GCT
3077 AGCGCT
ApaI 132 GGGCCC GGGCC^C
151 GGGCCC
Asp700I 851 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2362 GAATATGTTC
AvaI 111 CCCGGG C^[CT]CG[GA]G
148 CTCGGG
BanI 115 GGTACC G^G[CT][GA]CC
1444 GGTGCC
BclI 2255 TGATCA T^GATCA
2760 TGATCA
BglII 2613 AGATCT A^GATCT
3389 AGATCT
BlpI 252 GCTAAGC GC^T[ACGT]AGC
3067 GCTGAGC
Bpu1102I 252 GCTAAGC GC^T[ACGT]AGC
3067 GCTGAGC
BsaI 1594 GAGACC GGTCTC(1/5)
3097 GAGACC
BseRI 2624 CTCCTC GAGGAG(10/8)
2200 GAGGAG
BsgI 3161 CTGCAC GTGCAG(16/14)
724 GTGCAG
BsiEI 233 CGGTCG CG[GA][CT]^CG
237 CGGCCG
BsoBI 111 CCCGGG C^[CT]CG[GA]G
148 CTCGGG
BsrFI 231 GCCGGT [GA]^CCGG[CT]
239 GCCGGC
BstAPI 807 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
825 GCAAGGCATGC
CelII 252 GCTAAGC GC^T[ACGT]AGC
3067 GCTGAGC
Cfr10I 231 GCCGGT [GA]^CCGG[CT]
239 GCCGGC
DraII 121 GGGTCCC [GA]G^G[ACGT]CC[CT]
131 GGGGCCC
Eco47III 2738 AGCGCT AGC^GCT
3077 AGCGCT
EcoO109I 121 GGGTCCC [GA]G^G[ACGT]CC[CT]
131 GGGGCCC
EcoT14I 72 CCTTGG C^C[AT][AT]GG
2371 CCATGG
FauI 135 CCCGC CCCGC(4/6)
261 GCGGG
FbaI 2255 TGATCA T^GATCA
2760 TGATCA
HaeII 2738 AGCGCT [GA]GCGC^[CT]
3077 AGCGCT
SacI 786 GAGCTC GAGCT^C
1708 GAGCTC
SspI 2269 AATATT AAT^ATT
3527 AATATT
StyI 72 CCTTGG C^C[AT][AT]GG
2371 CCATGG
XmnI 851 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2362 GAATATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI Eco81I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII