##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AccIII AgeI AhdI
AocI AscI AspEI AspI AvrII
BbrPI BclI BlnI BlpI Bpu1102I
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
DraIII DrdI Eam1105I Eco81I EcoNI
FbaI FseI HincII HindII HpaI
MluI MroI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RcaI SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI TfiI Tth111I
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 832 GGTACC G^GTACC
AclI 2648 AACGTT AA^CGTT
AflII 3360 CTTAAG C^TTAAG
Alw44I 1616 GTGCAC G^TGCAC
ApaLI 1616 GTGCAC G^TGCAC
AseI 2677 ATTAAT AT^TAAT
AsnI 2677 ATTAAT AT^TAAT
Asp700I 1048 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 832 GGTACC G^GTACC
BamHI 2542 GGATCC G^GATCC
BbvCI 217 CCTCAGC CCTCAGC(-5/-2)
BcgI 1906 CGAAGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3360 CTTAAG C^TTAAG
BglII 2945 AGATCT A^GATCT
BsaAI 907 CACGTA [CT]AC^GT[GA]
BsaBI 1085 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 410 GAGACC GGTCTC(1/5)
BsaWI 1504 TCCGGT [AT]^CCGG[AT]
BsgI 1628 CTGCAC GTGCAG(16/14)
BspLU11I 3029 ACATGT A^CATGT
BssHII 318 GCGCGC G^CGCGC
BssSI 854 CTCGTG CACGAG(-5/-1)
BstEII 952 GGTGACC G^GT[ACGT]ACC
BstPI 952 GGTGACC G^GT[ACGT]ACC
CpoI 163 CGGACCG CG^G[AT]CCG
EcoO65I 952 GGTGACC G^GT[ACGT]ACC
EcoRI 3075 GAATTC G^AATTC
EcoRV 2256 GATATC GAT^ATC
EcoT14I 696 CCATGG C^C[AT][AT]GG
EcoT22I 2304 ATGCAT ATGCA^T
HindIII 2374 AAGCTT A^AGCTT
HinfI 1226 GACTC G^A[ACGT]TC
KpnI 832 GGTACC GGTAC^C
KspI 2010 CCGCGG CCGC^GG
MamI 1085 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2854 CAATTG C^AATTG
MunI 2854 CAATTG C^AATTG
NcoI 696 CCATGG C^CATGG
NsiI 2304 ATGCAT ATGCA^T
PaeR7I 984 CTCGAG C^TCGAG
PflMI 1270 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1226 GACTC GAGTC(4/5)
PpuMI 125 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2677 ATTAAT AT^TAAT
Psp1406I 2648 AACGTT AA^CGTT
RsrII 163 CGGACCG CG^G[AT]CCG
SacII 2010 CCGCGG CCGC^GG
ScaI 2271 AGTACT AGT^ACT
SspI 3273 AATATT AAT^ATT
StuI 1735 AGGCCT AGG^CCT
StyI 696 CCATGG C^C[AT][AT]GG
Van91I 1270 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2942 TCTAGA T^CTAGA
XhoI 984 CTCGAG C^TCGAG
XmnI 1048 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Aor51HI 604 AGCGCT AGC^GCT
2113 AGCGCT
ApaI 863 GGGCCC GGGCC^C
1464 GGGCCC
BalI 693 TGGCCA TGG^CCA
1933 TGGCCA
BbsI 616 GAAGAC GAAGAC(2/6)
1562 GTCTTC
BciVI 2591 GGATAC GTATCC(6/5)
2207 GTATCC
BpuAI 616 GAAGAC GAAGAC(2/6)
1562 GTCTTC
BstXI 1454 CCACTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1758 CCAACGGGCTGG
EagI 325 CGGCCG C^GGCCG
1162 CGGCCG
EclXI 325 CGGCCG C^GGCCG
1162 CGGCCG
Eco47III 604 AGCGCT AGC^GCT
2113 AGCGCT
Eco52I 325 CGGCCG C^GGCCG
1162 CGGCCG
HgaI 901 GACGC GACGC(5/10)
919 GACGC
MluNI 693 TGGCCA TGG^CCA
1933 TGGCCA
MscI 693 TGGCCA TGG^CCA
1933 TGGCCA
NaeI 303 GCCGGC GCC^GGC
1623 GCCGGC
NgoMIV 303 GCCGGC G^CCGGC
1623 GCCGGC
PvuII 1642 CAGCTG CAG^CTG
1775 CAGCTG
SacI 85 GAGCTC GAGCT^C
1034 GAGCTC
SphI 960 GCATGC GCATG^C
2306 GCATGC
XcmI 1115 CCAGGCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1593 CCAACGCCAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII AviII BanI
BanII BbeI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI