##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AlwNI Aor51HI ApaI AscI
Asp700I Asp718I AspI AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BglI BlnI
BlpI Bpu1102I BpuAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BstEII
BstPI BstUI CelII Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FauI
FbaI FseI HaeII HapII Hin1I
HincII HindII HpaI HpaII KasI
KpnI KspI MluI MroI MspA1I
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SspBI
StuI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 623 GTATAC GT^[AC][GT]AC
AciI 2596 GCGG CCGC(-3/-1)
AclI 438 AACGTT AA^CGTT
AflII 3456 CTTAAG C^TTAAG
AhdI 2364 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2294 CCTCAGG CC^T[ACGT]AGG
AseI 128 ATTAAT AT^TAAT
AsnI 128 ATTAAT AT^TAAT
AspEI 2364 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1507 CCCGAG C^[CT]CG[GA]G
AviII 3436 TGCGCA TGC^GCA
BalI 1341 TGGCCA TGG^CCA
BanII 2905 GAGCCC G[GA]GC[CT]^C
BfrI 3456 CTTAAG C^TTAAG
BglII 1063 AGATCT A^GATCT
BpmI 3646 CTGGAG CTGGAG(16/14)
BsaAI 1032 TACGTG [CT]AC^GT[GA]
BsaBI 3138 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 3254 CTGCAC GTGCAG(16/14)
BsoBI 1507 CCCGAG C^[CT]CG[GA]G
BspHI 3121 TCATGA T^CATGA
BssKI 1147 CCAGG ^CC[ACGT]GG
Bst1107I 623 GTATAC GTA^TAC
BstAPI 2429 GCAAGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 1147 CCAGG CC^[AT]GG
BstZ17I 623 GTATAC GTA^TAC
Bsu36I 2294 CCTCAGG CC^T[ACGT]AGG
CfoI 3437 GCGC GCG^C
DsaI 1088 CCATGG C^C[GA][CT]GG
EaeI 1341 TGGCCA [CT]^GGCC[GA]
Eam1105I 2364 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2294 CCTCAGG CC^T[ACGT]AGG
EcoNI 113 CCTCCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1727 GAATTC G^AATTC
EcoRII 1147 CCAGG ^CC[AT]GG
EcoT14I 1088 CCATGG C^C[AT][AT]GG
EcoT22I 3199 ATGCAT ATGCA^T
FspI 3436 TGCGCA TGC^GCA
HgaI 931 GACGC GACGC(5/10)
HhaI 3437 GCGC GCG^C
HinP1I 3437 GCGC G^CGC
HindIII 3422 AAGCTT A^AGCTT
MamI 3138 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1493 CAATTG C^AATTG
MluNI 1341 TGGCCA TGG^CCA
MscI 1341 TGGCCA TGG^CCA
MunI 1493 CAATTG C^AATTG
MvaI 1147 CCAGG CC^[AT]GG
NcoI 1088 CCATGG C^CATGG
NsiI 3199 ATGCAT ATGCA^T
PshBI 128 ATTAAT AT^TAAT
Psp1406I 438 AACGTT AA^CGTT
PstI 2613 CTGCAG CTGCA^G
RcaI 3121 TCATGA T^CATGA
SapI 1482 GAAGAGC GCTCTTC(1/4)
ScrFI 1147 CCAGG CC^[ACGT]GG
SpeI 3403 ACTAGT A^CTAGT
StyI 1088 CCATGG C^C[AT][AT]GG
SwaI 3361 ATTTAAAT ATTT^AAAT
XbaI 1731 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 2697 ACACGT A^C[GA][CT]GT
2855 ACGTGT
Alw44I 1000 GTGCAC G^TGCAC
2608 GTGCAC
ApaLI 1000 GTGCAC G^TGCAC
2608 GTGCAC
AspHI 1000 GTGCAC G[AT]GC[AT]^C
2608 GTGCAC
AvaII 467 GGACC G^G[AT]CC
1916 GGTCC
BbvI 2679 GCAGC GCAGC(8/12)
1200 GCTGC
BciVI 2712 GGATAC GTATCC(6/5)
1503 GTATCC
BsaJI 1088 CCATGG C^C[ACGT][ACGT]GG
1146 CCCAGG
BseRI 2263 CTCCTC GAGGAG(10/8)
1093 GAGGAG
BsiHKAI 1000 GTGCAC G[AT]GC[AT]^C
2608 GTGCAC
BsmFI 466 GGGAC GGGAC(10/14)
2540 GGGAC
BssSI 1612 CACGAG CACGAG(-5/-1)
1195 CTCGTG
BstXI 1266 CCATTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2048 CCAGAGGTTTGG
Cfr13I 467 GGACC G^G[ACGT]CC
1916 GGTCC
DraII 466 GGGACCT [GA]G^G[ACGT]CC[CT]
1915 AGGTCCT
EcoO109I 466 GGGACCT [GA]G^G[ACGT]CC[CT]
1915 AGGTCCT
NlaIV 466 GGGACC GG[ACGT]^[ACGT]CC
2904 GGAGCC
PpuMI 466 GGGACCT [GA]G^G[AT]CC[CT]
1915 AGGTCCT
Sau96I 467 GGACC G^G[ACGT]CC
1916 GGTCC
SfcI 2247 CTACAG C^T[GA][CT]AG
2613 CTGCAG
SmlI 226 CTCAAG C^T[CT][GA]AG
3456 CTTAAG
TseI 1200 GCTGC G^C[AT]GC
2679 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BfaI BmyI BsiYI BslI BsmAI
BsmI Bsp1286I BsrDI BsrI BstBI
BstF5I BstYI Cac8I DdeI DpnII
DraI EarI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MwoI NdeII
NlaIII NspI NspV PleI RsaI
Sau3AI SfaNI SfuI SphI SspI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XhoII