##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AccII AccIII AclI AcsI AflIII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI AviII AvrII BbrPI
BcgI BglI BlnI BsaAI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraI EagI
EclXI Eco52I EcoO65I EcoRI EcoT22I
FseI FspI HgaI HincII HindII
HindIII HpaI KspI MluI MroI
MvnI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2857 GACGTC GACGT^C
AflII 3507 CTTAAG C^TTAAG
AhdI 3306 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3182 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1089 AGCGCT AGC^GCT
ApaI 1006 GGGCCC GGGCC^C
AspEI 3306 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3073 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 433 GGATCC G^GATCC
BbeI 1053 GGCGCC GGCGC^C
BbsI 1367 GTCTTC GAAGAC(2/6)
BbvCI 3477 GCTGAGG CCTCAGC(-5/-2)
BfrI 3507 CTTAAG C^TTAAG
BglII 3436 AGATCT A^GATCT
BlpI 2223 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2223 GCTGAGC GC^T[ACGT]AGC
BpuAI 1367 GTCTTC GAAGAC(2/6)
BsaBI 2582 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 1880 ACCTGC ACCTGC(4/8)
Bsu36I 3182 CCTTAGG CC^T[ACGT]AGG
CelII 2223 GCTGAGC GC^T[ACGT]AGC
DraIII 3242 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 572 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2996 CCATGG C^C[GA][CT]GG
Eam1105I 3306 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1089 AGCGCT AGC^GCT
Eco81I 3182 CCTTAGG CC^T[ACGT]AGG
EcoRV 1427 GATATC GAT^ATC
KasI 1053 GGCGCC G^GCGCC
MamI 2582 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1634 CAATTG C^AATTG
MunI 1634 CAATTG C^AATTG
NarI 1053 GGCGCC GG^CGCC
NcoI 2996 CCATGG C^CATGG
PaeR7I 3090 CTCGAG C^TCGAG
PflFI 3073 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 2273 GAGCTC GAGCT^C
ScaI 898 AGTACT AGT^ACT
SfoI 1053 GGCGCC GGC^GCC
SpeI 3423 ACTAGT A^CTAGT
SphI 2795 GCATGC GCATG^C
Tth111I 3073 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3090 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 1404 GGTACC G^GTACC
3188 GGTACC
AcyI 1053 GGCGCC G[GA]^CG[CT]C
2857 GACGTC
Asp700I 2363 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2924 GAAGGTATTC
Asp718I 1404 GGTACC G^GTACC
3188 GGTACC
BciVI 2942 GGATAC GTATCC(6/5)
3365 GGATAC
BclI 2146 TGATCA T^GATCA
3157 TGATCA
BsaHI 1053 GGCGCC G[GA]^CG[CT]C
2857 GACGTC
BsaI 2626 GAGACC GGTCTC(1/5)
2331 GGTCTC
BsgI 2356 GTGCAG GTGCAG(16/14)
2753 GTGCAG
BsmBI 1438 CGTCTC CGTCTC(1/5)
2855 GAGACG
BspHI 151 TCATGA T^CATGA
1684 TCATGA
EarI 3392 CTCTTC CTCTTC(1/4)
381 GAAGAG
EcoNI 3178 CCTGCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3356 CCTCCTCCAGG
FauI 1014 CCCGC CCCGC(4/6)
920 GCGGG
FbaI 2146 TGATCA T^GATCA
3157 TGATCA
Hin1I 1053 GGCGCC G[GA]^CG[CT]C
2857 GACGTC
KpnI 1404 GGTACC GGTAC^C
3188 GGTACC
Ksp632I 3392 CTCTTC CTCTTC(1/4)
381 GAAGAG
MaeII 1200 ACGT A^CGT
2858 ACGT
NheI 346 GCTAGC G^CTAGC
3322 GCTAGC
NspI 2795 GCATGC [GA]CATG^[CT]
3499 ACATGC
RcaI 151 TCATGA T^CATGA
1684 TCATGA
SexAI 332 ACCAGGT A^CC[AT]GGT
1801 ACCAGGT
XbaI 1918 TCTAGA T^CTAGA
2668 TCTAGA
XmnI 2363 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2924 GAAGGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII