################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AccII AccIII AclI AcsI AflIII AgeI Alw44I ApaLI ApoI AscI AseI AsnI AviII AvrII BbrPI BcgI BglI BlnI BsaAI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I EcoRI EcoT22I FseI FspI HgaI HincII HindII HindIII HpaI KspI MluI MroI MvnI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SspI SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 2857 GACGTC GACGT^C AflII 3507 CTTAAG C^TTAAG AhdI 3306 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3182 CCTTAGG CC^T[ACGT]AGG Aor51HI 1089 AGCGCT AGC^GCT ApaI 1006 GGGCCC GGGCC^C AspEI 3306 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3073 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 433 GGATCC G^GATCC BbeI 1053 GGCGCC GGCGC^C BbsI 1367 GTCTTC GAAGAC(2/6) BbvCI 3477 GCTGAGG CCTCAGC(-5/-2) BfrI 3507 CTTAAG C^TTAAG BglII 3436 AGATCT A^GATCT BlpI 2223 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2223 GCTGAGC GC^T[ACGT]AGC BpuAI 1367 GTCTTC GAAGAC(2/6) BsaBI 2582 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspMI 1880 ACCTGC ACCTGC(4/8) Bsu36I 3182 CCTTAGG CC^T[ACGT]AGG CelII 2223 GCTGAGC GC^T[ACGT]AGC DraIII 3242 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 572 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2996 CCATGG C^C[GA][CT]GG Eam1105I 3306 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1089 AGCGCT AGC^GCT Eco81I 3182 CCTTAGG CC^T[ACGT]AGG EcoRV 1427 GATATC GAT^ATC KasI 1053 GGCGCC G^GCGCC MamI 2582 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1634 CAATTG C^AATTG MunI 1634 CAATTG C^AATTG NarI 1053 GGCGCC GG^CGCC NcoI 2996 CCATGG C^CATGG PaeR7I 3090 CTCGAG C^TCGAG PflFI 3073 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 2273 GAGCTC GAGCT^C ScaI 898 AGTACT AGT^ACT SfoI 1053 GGCGCC GGC^GCC SpeI 3423 ACTAGT A^CTAGT SphI 2795 GCATGC GCATG^C Tth111I 3073 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3090 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 1404 GGTACC G^GTACC 3188 GGTACC AcyI 1053 GGCGCC G[GA]^CG[CT]C 2857 GACGTC Asp700I 2363 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2924 GAAGGTATTC Asp718I 1404 GGTACC G^GTACC 3188 GGTACC BciVI 2942 GGATAC GTATCC(6/5) 3365 GGATAC BclI 2146 TGATCA T^GATCA 3157 TGATCA BsaHI 1053 GGCGCC G[GA]^CG[CT]C 2857 GACGTC BsaI 2626 GAGACC GGTCTC(1/5) 2331 GGTCTC BsgI 2356 GTGCAG GTGCAG(16/14) 2753 GTGCAG BsmBI 1438 CGTCTC CGTCTC(1/5) 2855 GAGACG BspHI 151 TCATGA T^CATGA 1684 TCATGA EarI 3392 CTCTTC CTCTTC(1/4) 381 GAAGAG EcoNI 3178 CCTGCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3356 CCTCCTCCAGG FauI 1014 CCCGC CCCGC(4/6) 920 GCGGG FbaI 2146 TGATCA T^GATCA 3157 TGATCA Hin1I 1053 GGCGCC G[GA]^CG[CT]C 2857 GACGTC KpnI 1404 GGTACC GGTAC^C 3188 GGTACC Ksp632I 3392 CTCTTC CTCTTC(1/4) 381 GAAGAG MaeII 1200 ACGT A^CGT 2858 ACGT NheI 346 GCTAGC G^CTAGC 3322 GCTAGC NspI 2795 GCATGC [GA]CATG^[CT] 3499 ACATGC RcaI 151 TCATGA T^CATGA 1684 TCATGA SexAI 332 ACCAGGT A^CC[AT]GGT 1801 ACCAGGT XbaI 1918 TCTAGA T^CTAGA 2668 TCTAGA XmnI 2363 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2924 GAAGGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII