##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AspI BamHI BciVI BclI BfrI
BglII BsaBI BsaWI BseAI BsiWI
BspEI BspHI BsrBI BsrDI BsrFI
BssSI Bst1107I BstEII BstPI BstZ17I
Cfr10I CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI HgaI HpaI
KpnI KspI MamI MluI MroI
NaeI NdeI NgoMIV NotI NruI
NsiI PacI PflFI PflMI PinAI
PmeI Psp1406I RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1027 GACGTC GACGT^C
AcsI 241 GAATTC [GA]^AATT[CT]
AflIII 1004 ACATGT A^C[GA][CT]GT
AhdI 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1404 GGGCCC GGGCC^C
ApoI 241 GAATTC [GA]^AATT[CT]
AseI 222 ATTAAT AT^TAAT
AsnI 222 ATTAAT AT^TAAT
AspEI 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3015 TGCGCA TGC^GCA
BbeI 18 GGCGCC GGCGC^C
BbrPI 2184 CACGTG CAC^GTG
BcgI 1284 GCAGGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 3326 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3326 GCTAAGC GC^T[ACGT]AGC
BsgI 1095 CTGCAC GTGCAG(16/14)
BsiEI 156 CGATCG CG[GA][CT]^CG
BsmI 2752 GAATGC GAATGC(1/-1)
Bsp1407I 261 TGTACA T^GTACA
BspDI 383 ATCGAT AT^CGAT
BspLU11I 1004 ACATGT A^CATGT
BspMI 2171 GCAGGT ACCTGC(4/8)
BsrGI 261 TGTACA T^GTACA
BssHII 2836 GCGCGC G^CGCGC
BstAPI 1199 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 359 TTCGAA TT^CGAA
CelII 3326 GCTAAGC GC^T[ACGT]AGC
ClaI 383 ATCGAT AT^CGAT
DrdI 1807 GACAATCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 241 GAATTC G^AATTC
FspI 3015 TGCGCA TGC^GCA
HaeII 18 GGCGCC [GA]GCGC^[CT]
HincII 2644 GTCAAC GT[CT]^[GA]AC
HindII 2644 GTCAAC GT[CT]^[GA]AC
HindIII 3306 AAGCTT A^AGCTT
KasI 18 GGCGCC G^GCGCC
MfeI 1203 CAATTG C^AATTG
MunI 1203 CAATTG C^AATTG
NarI 18 GGCGCC GG^CGCC
NheI 3243 GCTAGC G^CTAGC
NspV 359 TTCGAA TT^CGAA
PaeR7I 896 CTCGAG C^TCGAG
PmaCI 2184 CACGTG CAC^GTG
PmlI 2184 CACGTG CAC^GTG
PpuMI 194 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3086 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 222 ATTAAT AT^TAAT
PvuI 156 CGATCG CGAT^CG
SfoI 18 GGCGCC GGC^GCC
SfuI 359 TTCGAA TT^CGAA
SmaI 1875 CCCGGG CCC^GGG
SspBI 261 TGTACA T^GTACA
SspI 3436 AATATT AAT^ATT
StuI 2802 AGGCCT AGG^CCT
XbaI 2392 TCTAGA T^CTAGA
XhoI 896 CTCGAG C^TCGAG
XmaCI 1875 CCCGGG C^CCGGG
XmaI 1875 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1045 GTCTAC GT^[AC][GT]AC
2319 GTAGAC
AccII 63 CGCG CG^CG
2837 CGCG
AcyI 18 GGCGCC G[GA]^CG[CT]C
1027 GACGTC
Asp700I 1921 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2960 GAAACAATTC
BalI 209 TGGCCA TGG^CCA
2913 TGGCCA
BbsI 592 GAAGAC GAAGAC(2/6)
648 GAAGAC
BpuAI 592 GAAGAC GAAGAC(2/6)
648 GAAGAC
BsaAI 961 CACGTA [CT]AC^GT[GA]
2184 CACGTG
BsaHI 18 GGCGCC G[GA]^CG[CT]C
1027 GACGTC
BsaI 658 GGTCTC GGTCTC(1/5)
916 GGTCTC
BsmBI 510 GAGACG CGTCTC(1/5)
765 GAGACG
BstUI 63 CGCG CG^CG
2837 CGCG
BstXI 419 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
839 CCAAAATGCTGG
DsaI 1146 CCATGG C^C[GA][CT]GG
2910 CCATGG
Hin1I 18 GGCGCC G[GA]^CG[CT]C
1027 GACGTC
MluNI 209 TGGCCA TGG^CCA
2913 TGGCCA
MscI 209 TGGCCA TGG^CCA
2913 TGGCCA
MvnI 63 CGCG CG^CG
2837 CGCG
NcoI 1146 CCATGG C^CATGG
2910 CCATGG
NspI 956 ACATGC [GA]CATG^[CT]
1004 ACATGT
SacI 376 GAGCTC GAGCT^C
809 GAGCTC
XcmI 2916 CCAAGGTACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3262 CCATCCTGACTGTGG
XmnI 1921 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2960 GAAACAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII AvrII
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII