################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI Asp718I AspI BamHI BciVI BclI BfrI BglII BsaBI BsaWI BseAI BsiWI BspEI BspHI BsrBI BsrDI BsrFI BssSI Bst1107I BstEII BstPI BstZ17I Cfr10I CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI HgaI HpaI KpnI KspI MamI MluI MroI NaeI NdeI NgoMIV NotI NruI NsiI PacI PflFI PflMI PinAI PmeI Psp1406I RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 1027 GACGTC GACGT^C AcsI 241 GAATTC [GA]^AATT[CT] AflIII 1004 ACATGT A^C[GA][CT]GT AhdI 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1404 GGGCCC GGGCC^C ApoI 241 GAATTC [GA]^AATT[CT] AseI 222 ATTAAT AT^TAAT AsnI 222 ATTAAT AT^TAAT AspEI 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3015 TGCGCA TGC^GCA BbeI 18 GGCGCC GGCGC^C BbrPI 2184 CACGTG CAC^GTG BcgI 1284 GCAGGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 3326 GCTAAGC GC^T[ACGT]AGC Bpu1102I 3326 GCTAAGC GC^T[ACGT]AGC BsgI 1095 CTGCAC GTGCAG(16/14) BsiEI 156 CGATCG CG[GA][CT]^CG BsmI 2752 GAATGC GAATGC(1/-1) Bsp1407I 261 TGTACA T^GTACA BspDI 383 ATCGAT AT^CGAT BspLU11I 1004 ACATGT A^CATGT BspMI 2171 GCAGGT ACCTGC(4/8) BsrGI 261 TGTACA T^GTACA BssHII 2836 GCGCGC G^CGCGC BstAPI 1199 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 359 TTCGAA TT^CGAA CelII 3326 GCTAAGC GC^T[ACGT]AGC ClaI 383 ATCGAT AT^CGAT DrdI 1807 GACAATCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 241 GAATTC G^AATTC FspI 3015 TGCGCA TGC^GCA HaeII 18 GGCGCC [GA]GCGC^[CT] HincII 2644 GTCAAC GT[CT]^[GA]AC HindII 2644 GTCAAC GT[CT]^[GA]AC HindIII 3306 AAGCTT A^AGCTT KasI 18 GGCGCC G^GCGCC MfeI 1203 CAATTG C^AATTG MunI 1203 CAATTG C^AATTG NarI 18 GGCGCC GG^CGCC NheI 3243 GCTAGC G^CTAGC NspV 359 TTCGAA TT^CGAA PaeR7I 896 CTCGAG C^TCGAG PmaCI 2184 CACGTG CAC^GTG PmlI 2184 CACGTG CAC^GTG PpuMI 194 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 3086 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 222 ATTAAT AT^TAAT PvuI 156 CGATCG CGAT^CG SfoI 18 GGCGCC GGC^GCC SfuI 359 TTCGAA TT^CGAA SmaI 1875 CCCGGG CCC^GGG SspBI 261 TGTACA T^GTACA SspI 3436 AATATT AAT^ATT StuI 2802 AGGCCT AGG^CCT XbaI 2392 TCTAGA T^CTAGA XhoI 896 CTCGAG C^TCGAG XmaCI 1875 CCCGGG C^CCGGG XmaI 1875 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1045 GTCTAC GT^[AC][GT]AC 2319 GTAGAC AccII 63 CGCG CG^CG 2837 CGCG AcyI 18 GGCGCC G[GA]^CG[CT]C 1027 GACGTC Asp700I 1921 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2960 GAAACAATTC BalI 209 TGGCCA TGG^CCA 2913 TGGCCA BbsI 592 GAAGAC GAAGAC(2/6) 648 GAAGAC BpuAI 592 GAAGAC GAAGAC(2/6) 648 GAAGAC BsaAI 961 CACGTA [CT]AC^GT[GA] 2184 CACGTG BsaHI 18 GGCGCC G[GA]^CG[CT]C 1027 GACGTC BsaI 658 GGTCTC GGTCTC(1/5) 916 GGTCTC BsmBI 510 GAGACG CGTCTC(1/5) 765 GAGACG BstUI 63 CGCG CG^CG 2837 CGCG BstXI 419 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 839 CCAAAATGCTGG DsaI 1146 CCATGG C^C[GA][CT]GG 2910 CCATGG Hin1I 18 GGCGCC G[GA]^CG[CT]C 1027 GACGTC MluNI 209 TGGCCA TGG^CCA 2913 TGGCCA MscI 209 TGGCCA TGG^CCA 2913 TGGCCA MvnI 63 CGCG CG^CG 2837 CGCG NcoI 1146 CCATGG C^CATGG 2910 CCATGG NspI 956 ACATGC [GA]CATG^[CT] 1004 ACATGT SacI 376 GAGCTC GAGCT^C 809 GAGCTC XcmI 2916 CCAAGGTACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3262 CCATCCTGACTGTGG XmnI 1921 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2960 GAAACAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII AvrII BanI BanII BbvCI BbvI BcnI BfaI BglI BlnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII