##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I AspI AviII BciVI
BclI BfrI BglII BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eco47III EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1156 GGTACC G^GTACC
AccI 2402 GTCTAC GT^[AC][GT]AC
AflIII 2844 ACGTGT A^C[GA][CT]GT
AhdI 3407 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1156 GGTACC G^GTACC
AspEI 3407 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1820 GGCGCC GGCGC^C
BcgI 535 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2827 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2827 GCTCAGC GC^T[ACGT]AGC
BsiEI 2363 CGGCCG CG[GA][CT]^CG
BsrBI 1977 CCGCTC CCGCTC(-3/-3)
CelII 2827 GCTCAGC GC^T[ACGT]AGC
DrdI 2543 GACGTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2363 CGGCCG C^GGCCG
Eam1105I 3407 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2363 CGGCCG C^GGCCG
Eco52I 2363 CGGCCG C^GGCCG
HindIII 149 AAGCTT A^AGCTT
KasI 1820 GGCGCC G^GCGCC
KpnI 1156 GGTACC GGTAC^C
KspI 1919 CCGCGG CCGC^GG
NarI 1820 GGCGCC GG^CGCC
PpuMI 2688 GGGTCCC [GA]G^G[AT]CC[CT]
SacII 1919 CCGCGG CCGC^GG
SapI 2803 GCTCTTC GCTCTTC(1/4)
SfoI 1820 GGCGCC GGC^GCC
SmlI 2786 CTCAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvrII 2747 CCTAGG C^CTAGG
2889 CCTAGG
BbrPI 916 CACGTG CAC^GTG
940 CACGTG
BbsI 2271 GAAGAC GAAGAC(2/6)
3130 GAAGAC
BbvCI 546 CCTCAGC CCTCAGC(-5/-2)
1221 GCTGAGG
BlnI 2747 CCTAGG C^CTAGG
2889 CCTAGG
BpuAI 2271 GAAGAC GAAGAC(2/6)
3130 GAAGAC
BstAPI 2306 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2569 GCACAACCTGC
BstEII 1460 GGTGACC G^GT[ACGT]ACC
3222 GGTGACC
BstPI 1460 GGTGACC G^GT[ACGT]ACC
3222 GGTGACC
DraIII 1997 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
1999 CACTGTGTG
EarI 1790 CTCTTC CTCTTC(1/4)
2804 CTCTTC
EcoO65I 1460 GGTGACC G^GT[ACGT]ACC
3222 GGTGACC
HaeII 1125 AGCGCC [GA]GCGC^[CT]
1820 GGCGCC
Ksp632I 1790 CTCTTC CTCTTC(1/4)
2804 CTCTTC
PflMI 668 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCACCCGTTGG
PmaCI 916 CACGTG CAC^GTG
940 CACGTG
PmlI 916 CACGTG CAC^GTG
940 CACGTG
SmaI 523 CCCGGG CCC^GGG
3256 CCCGGG
Van91I 668 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCACCCGTTGG
XmaCI 523 CCCGGG C^CCGGG
3256 CCCGGG
XmaI 523 CCCGGG C^CCGGG
3256 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XcmI XhoII