##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I AocI ApaI ApaLI AscI
Asp700I Asp718I AvrII BbrPI BciVI
BfrI BlnI BsiWI BsmI BspDI
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI EarI Eco81I EcoRI
EcoT22I FseI HpaI KpnI Ksp632I
KspI MfeI MluI MunI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SnaBI SpeI Sse8387I
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 512 GACGTC GACGT^C
AccIII 957 TCCGGA T^CCGGA
AflIII 3469 ACATGT A^C[GA][CT]GT
AhdI 2272 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2092 AGCGCT AGC^GCT
AseI 73 ATTAAT AT^TAAT
AsnI 73 ATTAAT AT^TAAT
AspEI 2272 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3462 CCCGGG C^[CT]CG[GA]G
AviII 372 TGCGCA TGC^GCA
BamHI 1271 GGATCC G^GATCC
BbeI 1089 GGCGCC GGCGC^C
BglI 2306 GCCTTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 561 AGATCT A^GATCT
BsaAI 422 TACGTG [CT]AC^GT[GA]
BseAI 957 TCCGGA T^CCGGA
BsiEI 2313 CGGCCG CG[GA][CT]^CG
BsmBI 944 GAGACG CGTCTC(1/5)
BsoBI 3462 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3487 TGTACA T^GTACA
BspEI 957 TCCGGA T^CCGGA
BspLU11I 3469 ACATGT A^CATGT
BsrGI 3487 TGTACA T^GTACA
BssHII 1804 GCGCGC G^CGCGC
BstEII 351 GGTCACC G^GT[ACGT]ACC
BstPI 351 GGTCACC G^GT[ACGT]ACC
DraI 1301 TTTAAA TTT^AAA
DrdI 3239 GACGGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 123 CCGTGG C^C[GA][CT]GG
EagI 2313 CGGCCG C^GGCCG
Eam1105I 2272 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2313 CGGCCG C^GGCCG
Eco47III 2092 AGCGCT AGC^GCT
Eco52I 2313 CGGCCG C^GGCCG
EcoO65I 351 GGTCACC G^GT[ACGT]ACC
EcoRV 3271 GATATC GAT^ATC
FspI 372 TGCGCA TGC^GCA
KasI 1089 GGCGCC G^GCGCC
MroI 957 TCCGGA T^CCGGA
NarI 1089 GGCGCC GG^CGCC
NdeI 2965 CATATG CA^TATG
PflMI 1399 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 73 ATTAAT AT^TAAT
SexAI 384 ACCAGGT A^CC[AT]GGT
SfoI 1089 GGCGCC GGC^GCC
SgrAI 1868 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3462 CCCGGG CCC^GGG
SmlI 203 CTCAAG C^T[CT][GA]AG
SspBI 3487 TGTACA T^GTACA
SspI 3348 AATATT AAT^ATT
StuI 574 AGGCCT AGG^CCT
Van91I 1399 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3462 CCCGGG C^CCGGG
XmaI 3462 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1453 AAATTC [GA]^AATT[CT]
2224 AAATTC
AlwNI 428 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
692 CAGATCCTG
ApoI 1453 AAATTC [GA]^AATT[CT]
2224 AAATTC
AspI 509 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1852 GACATCGTC
BbvCI 1933 CCTCAGC CCTCAGC(-5/-2)
410 GCTGAGG
BcgI 981 GCATGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2541 GCAAGGTGATCG
BlpI 2095 GCTCAGC GC^T[ACGT]AGC
2702 GCTGAGC
Bpu1102I 2095 GCTCAGC GC^T[ACGT]AGC
2702 GCTGAGC
BsaBI 163 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2138 GATATTGATC
BsrFI 312 GCCGGC [GA]^CCGG[CT]
1869 GCCGGC
BstXI 1731 CCAGGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2419 CCAGAGGAATGG
CelII 2095 GCTCAGC GC^T[ACGT]AGC
2702 GCTGAGC
Cfr10I 312 GCCGGC [GA]^CCGG[CT]
1869 GCCGGC
DraIII 2489 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3162 CACCCTGTG
EcoT14I 571 CCAAGG C^C[AT][AT]GG
1458 CCAAGG
MamI 163 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2138 GATATTGATC
NaeI 312 GCCGGC GCC^GGC
1869 GCCGGC
NgoMIV 312 GCCGGC G^CCGGC
1869 GCCGGC
PflFI 509 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1852 GACATCGTC
SphI 369 GCATGC GCATG^C
399 GCATGC
StyI 571 CCAAGG C^C[AT][AT]GG
1458 CCAAGG
Tth111I 509 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1852 GACATCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BalI BanI
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoNI
EcoO109I EcoRII FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII