################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI BalI BcgI BciVI BclI BfrI BsaBI BsaWI BseAI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV FbaI HindIII HpaI MamI MluNI MroI MscI NcoI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I RcaI SacI SalI SbfI SexAI SfuI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 320 GGTACC G^GTACC AccI 745 GTCTAC GT^[AC][GT]AC Asp718I 320 GGTACC G^GTACC AspHI 2723 GTGCTC G[AT]GC[AT]^C AviII 671 TGCGCA TGC^GCA AvrII 2623 CCTAGG C^CTAGG BamHI 1210 GGATCC G^GATCC BbrPI 3552 CACGTG CAC^GTG BglII 343 AGATCT A^GATCT BlnI 2623 CCTAGG C^CTAGG BsaAI 3552 CACGTG [CT]AC^GT[GA] BsiHKAI 2723 GTGCTC G[AT]GC[AT]^C BsiWI 548 CGTACG C^GTACG BspMI 1750 ACCTGC ACCTGC(4/8) CpoI 2344 CGGACCG CG^G[AT]CCG EcoT22I 3544 ATGCAT ATGCA^T FseI 970 GGCCGGCC GGCCGG^CC FspI 671 TGCGCA TGC^GCA KpnI 320 GGTACC GGTAC^C MfeI 2941 CAATTG C^AATTG MluI 3190 ACGCGT A^CGCGT MseI 779 TTAA T^TAA MunI 2941 CAATTG C^AATTG NsiI 3544 ATGCAT ATGCA^T NspI 3546 GCATGC [GA]CATG^[CT] PaeR7I 1243 CTCGAG C^TCGAG PmaCI 3552 CACGTG CAC^GTG PmlI 3552 CACGTG CAC^GTG PshAI 1286 GACCGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 3219 CGATCG CGAT^CG RsrII 2344 CGGACCG CG^G[AT]CCG SapI 594 GCTCTTC GCTCTTC(1/4) ScaI 1543 AGTACT AGT^ACT SfiI 3229 GGCCCCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 3546 GCATGC GCATG^C Tru9I 779 TTAA T^TAA Tsp509I 2942 AATT ^AATT XhoI 1243 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 870 GACGTC GACGT^C 1361 GACGTC AlwI 1211 GATCC GGATC(4/5) 1210 GGATC BbsI 1293 GTCTTC GAAGAC(2/6) 1377 GTCTTC BbvCI 1622 CCTCAGC CCTCAGC(-5/-2) 2392 CCTCAGC BfaI 622 CTAG C^TAG 2624 CTAG BpuAI 1293 GTCTTC GAAGAC(2/6) 1377 GTCTTC BsaI 1395 GAGACC GGTCTC(1/5) 3286 GGTCTC BsgI 1691 CTGCAC GTGCAG(16/14) 3194 GTGCAG BsmBI 2680 GAGACG CGTCTC(1/5) 2827 GAGACG BsrBI 2322 CCGCTC CCGCTC(-3/-3) 1836 GAGCGG BsrI 81 ACTGG ACTGG(1/-1) 2456 ACTGG BssSI 3210 CACGAG CACGAG(-5/-1) 3371 CACGAG BstAPI 199 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1445 GCAGCGGCTGC BstYI 343 AGATCT [GA]^GATC[CT] 1210 GGATCC EarI 577 CTCTTC CTCTTC(1/4) 595 CTCTTC HincII 241 GTCAAC GT[CT]^[GA]AC 2876 GTTGAC HindII 241 GTCAAC GT[CT]^[GA]AC 2876 GTTGAC Ksp632I 577 CTCTTC CTCTTC(1/4) 595 CTCTTC MaeI 622 CTAG C^TAG 2624 CTAG MflI 343 AGATCT [GA]^GATC[CT] 1210 GGATCC TaqI 402 TCGA T^CGA 1244 TCGA TthHB8I 402 TCGA T^CGA 1244 TCGA XcmI 2371 CCACGGGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2745 CCACCCGCGGAGTGG XhoII 343 AGATCT [GA]^GATC[CT] 1210 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwNI AocI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SgrAI SmaI SmlI StyI TseI Tsp45I TspRI Van91I XmaCI XmaI