##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI BalI BcgI BciVI BclI
BfrI BsaBI BsaWI BseAI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraI
DraIII DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV FbaI HindIII HpaI
MamI MluNI MroI MscI NcoI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I RcaI SacI SalI SbfI
SexAI SfuI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 320 GGTACC G^GTACC
AccI 745 GTCTAC GT^[AC][GT]AC
Asp718I 320 GGTACC G^GTACC
AspHI 2723 GTGCTC G[AT]GC[AT]^C
AviII 671 TGCGCA TGC^GCA
AvrII 2623 CCTAGG C^CTAGG
BamHI 1210 GGATCC G^GATCC
BbrPI 3552 CACGTG CAC^GTG
BglII 343 AGATCT A^GATCT
BlnI 2623 CCTAGG C^CTAGG
BsaAI 3552 CACGTG [CT]AC^GT[GA]
BsiHKAI 2723 GTGCTC G[AT]GC[AT]^C
BsiWI 548 CGTACG C^GTACG
BspMI 1750 ACCTGC ACCTGC(4/8)
CpoI 2344 CGGACCG CG^G[AT]CCG
EcoT22I 3544 ATGCAT ATGCA^T
FseI 970 GGCCGGCC GGCCGG^CC
FspI 671 TGCGCA TGC^GCA
KpnI 320 GGTACC GGTAC^C
MfeI 2941 CAATTG C^AATTG
MluI 3190 ACGCGT A^CGCGT
MseI 779 TTAA T^TAA
MunI 2941 CAATTG C^AATTG
NsiI 3544 ATGCAT ATGCA^T
NspI 3546 GCATGC [GA]CATG^[CT]
PaeR7I 1243 CTCGAG C^TCGAG
PmaCI 3552 CACGTG CAC^GTG
PmlI 3552 CACGTG CAC^GTG
PshAI 1286 GACCGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3219 CGATCG CGAT^CG
RsrII 2344 CGGACCG CG^G[AT]CCG
SapI 594 GCTCTTC GCTCTTC(1/4)
ScaI 1543 AGTACT AGT^ACT
SfiI 3229 GGCCCCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3546 GCATGC GCATG^C
Tru9I 779 TTAA T^TAA
Tsp509I 2942 AATT ^AATT
XhoI 1243 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 870 GACGTC GACGT^C
1361 GACGTC
AlwI 1211 GATCC GGATC(4/5)
1210 GGATC
BbsI 1293 GTCTTC GAAGAC(2/6)
1377 GTCTTC
BbvCI 1622 CCTCAGC CCTCAGC(-5/-2)
2392 CCTCAGC
BfaI 622 CTAG C^TAG
2624 CTAG
BpuAI 1293 GTCTTC GAAGAC(2/6)
1377 GTCTTC
BsaI 1395 GAGACC GGTCTC(1/5)
3286 GGTCTC
BsgI 1691 CTGCAC GTGCAG(16/14)
3194 GTGCAG
BsmBI 2680 GAGACG CGTCTC(1/5)
2827 GAGACG
BsrBI 2322 CCGCTC CCGCTC(-3/-3)
1836 GAGCGG
BsrI 81 ACTGG ACTGG(1/-1)
2456 ACTGG
BssSI 3210 CACGAG CACGAG(-5/-1)
3371 CACGAG
BstAPI 199 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1445 GCAGCGGCTGC
BstYI 343 AGATCT [GA]^GATC[CT]
1210 GGATCC
EarI 577 CTCTTC CTCTTC(1/4)
595 CTCTTC
HincII 241 GTCAAC GT[CT]^[GA]AC
2876 GTTGAC
HindII 241 GTCAAC GT[CT]^[GA]AC
2876 GTTGAC
Ksp632I 577 CTCTTC CTCTTC(1/4)
595 CTCTTC
MaeI 622 CTAG C^TAG
2624 CTAG
MflI 343 AGATCT [GA]^GATC[CT]
1210 GGATCC
TaqI 402 TCGA T^CGA
1244 TCGA
TthHB8I 402 TCGA T^CGA
1244 TCGA
XcmI 2371 CCACGGGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2745 CCACCCGCGGAGTGG
XhoII 343 AGATCT [GA]^GATC[CT]
1210 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI AocI ApaI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KspI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SgrAI SmaI
SmlI StyI TseI Tsp45I TspRI
Van91I XmaCI XmaI