##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AccIII AclI AflIII AgeI AlwNI
AocI Aor51HI AscI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BglI BlnI BsaAI
BsaWI BseAI BseRI BsiEI BsiWI
BspDI BspEI BspLU11I BsrBI BsrFI
BssSI BstAPI BstBI Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
FseI FspI HaeII KasI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I StuI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1198 GACGTC GACGT^C
Acc65I 2067 GGTACC G^GTACC
AcyI 1198 GACGTC G[GA]^CG[CT]C
ApaI 122 GGGCCC GGGCC^C
Asp718I 2067 GGTACC G^GTACC
AspI 2261 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 707 GAAGAC GAAGAC(2/6)
BciVI 3311 GTATCC GTATCC(6/5)
BclI 2505 TGATCA T^GATCA
BlpI 3181 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3181 GCTGAGC GC^T[ACGT]AGC
BpuAI 707 GAAGAC GAAGAC(2/6)
BsaHI 1198 GACGTC G[GA]^CG[CT]C
BsaI 830 GAGACC GGTCTC(1/5)
BsmBI 1371 GAGACG CGTCTC(1/5)
Bsp1407I 3209 TGTACA T^GTACA
BsrGI 3209 TGTACA T^GTACA
BstEII 1049 GGTAACC G^GT[ACGT]ACC
BstPI 1049 GGTAACC G^GT[ACGT]ACC
CelII 3181 GCTGAGC GC^T[ACGT]AGC
DsaI 62 CCGTGG C^C[GA][CT]GG
EarI 1717 GAAGAG CTCTTC(1/4)
EcoO65I 1049 GGTAACC G^GT[ACGT]ACC
EcoRV 1770 GATATC GAT^ATC
EcoT22I 2897 ATGCAT ATGCA^T
FbaI 2505 TGATCA T^GATCA
HgaI 15 GACGC GACGC(5/10)
Hin1I 1198 GACGTC G[GA]^CG[CT]C
KpnI 2067 GGTACC GGTAC^C
Ksp632I 1717 GAAGAG CTCTTC(1/4)
NheI 642 GCTAGC G^CTAGC
NsiI 2897 ATGCAT ATGCA^T
NspI 2110 GCATGC [GA]CATG^[CT]
PflFI 2261 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3316 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 247 GAGTC GAGTC(4/5)
PvuII 1910 CAGCTG CAG^CTG
SacI 997 GAGCTC GAGCT^C
SapI 1717 GAAGAGC GCTCTTC(1/4)
SfcI 739 CTATAG C^T[GA][CT]AG
SmaI 125 CCCGGG CCC^GGG
SpeI 2044 ACTAGT A^CTAGT
SphI 2110 GCATGC GCATG^C
SspBI 3209 TGTACA T^GTACA
Tth111I 2261 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3316 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3240 TCTAGA T^CTAGA
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 1198 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2323 GACATTTTGTC
Alw44I 1310 GTGCAC G^TGCAC
1963 GTGCAC
ApaLI 1310 GTGCAC G^TGCAC
1963 GTGCAC
AspEI 1198 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2323 GACATTTTGTC
AvaI 125 CCCGGG C^[CT]CG[GA]G
385 CTCGGG
BanI 67 GGTGCC G^G[CT][GA]CC
2067 GGTACC
BanII 122 GGGCCC G[GA]GC[CT]^C
997 GAGCTC
BglII 391 AGATCT A^GATCT
763 AGATCT
BpmI 3517 CTCCAG CTGGAG(16/14)
309 CTGGAG
BsaBI 1875 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2712 GATGGACATC
BsgI 342 GTGCAG GTGCAG(16/14)
1998 GTGCAG
BsmFI 2321 GGGAC GGGAC(10/14)
843 GTCCC
BsmI 675 GAATGC GAATGC(1/-1)
2307 GAATGC
BsoBI 125 CCCGGG C^[CT]CG[GA]G
385 CTCGGG
BspHI 974 TCATGA T^CATGA
3096 TCATGA
BsrDI 2570 GCAATG GCAATG(2/0)
3272 GCAATG
BssHII 82 GCGCGC G^CGCGC
84 GCGCGC
BstXI 1175 CCAGTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2041 CCAACTAGTTGG
DraII 33 GGGGCCT [GA]G^G[ACGT]CC[CT]
121 AGGGCCC
Eam1105I 1198 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2323 GACATTTTGTC
EcoO109I 33 GGGGCCT [GA]G^G[ACGT]CC[CT]
121 AGGGCCC
EcoRI 1190 GAATTC G^AATTC
1254 GAATTC
EcoT14I 2071 CCTTGG C^C[AT][AT]GG
2249 CCTTGG
FauI 230 CCCGC CCCGC(4/6)
102 GCGGG
HpaI 2585 GTTAAC GTT^AAC
2811 GTTAAC
MaeII 283 ACGT A^CGT
1199 ACGT
MamI 1875 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2712 GATGGACATC
RcaI 974 TCATGA T^CATGA
3096 TCATGA
StyI 2071 CCTTGG C^C[AT][AT]GG
2249 CCTTGG
SwaI 2299 ATTTAAAT ATTT^AAAT
3150 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI ApoI AseI
AsnI Asp700I AspHI BbvI BcnI
BfaI BfrI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI Bst1107I BstF5I BstNI
BstUI BstYI BstZ17I Cac8I CfoI
Cfr13I DdeI DpnII DraI EcoNI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI