##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrDI BssHII BssSI BstBI BstEII
BstPI BstXI BstYI Bsu36I CelII
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FseI FspI HpaI
KpnI MamI MfeI MflI MluNI
MroI MscI MunI NcoI NdeI
NheI NotI NruI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SphI Sse8387I
StuI SwaI TaqI Tth111I TthHB8I
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 671 GTATAC GT^[AC][GT]AC
AflIII 1701 ACGCGT A^C[GA][CT]GT
AlwI 1518 GATCC GGATC(4/5)
AseI 2559 ATTAAT AT^TAAT
AsnI 2559 ATTAAT AT^TAAT
Asp700I 1740 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 87 GGCGCC G^G[CT][GA]CC
BanII 227 GGGCTC G[GA]GC[CT]^C
BbeI 87 GGCGCC GGCGC^C
BclI 561 TGATCA T^GATCA
BsaAI 2592 TACGTA [CT]AC^GT[GA]
BsaI 2858 GGTCTC GGTCTC(1/5)
BsgI 3403 CTGCAC GTGCAG(16/14)
BsiEI 138 CGGTCG CG[GA][CT]^CG
Bsp1407I 3249 TGTACA T^GTACA
BspHI 3180 TCATGA T^CATGA
BsrFI 280 GCCGGC [GA]^CCGG[CT]
BsrGI 3249 TGTACA T^GTACA
Bst1107I 671 GTATAC GTA^TAC
BstAPI 650 GCAATACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 671 GTATAC GTA^TAC
Cfr10I 280 GCCGGC [GA]^CCGG[CT]
DrdI 241 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2681 GAATTC G^AATTC
EcoRV 971 GATATC GAT^ATC
FbaI 561 TGATCA T^GATCA
HaeII 87 GGCGCC [GA]GCGC^[CT]
HincII 2232 GTTGAC GT[CT]^[GA]AC
HindII 2232 GTTGAC GT[CT]^[GA]AC
KasI 87 GGCGCC G^GCGCC
MaeII 2593 ACGT A^CGT
MluI 1701 ACGCGT A^CGCGT
NaeI 280 GCCGGC GCC^GGC
NarI 87 GGCGCC GG^CGCC
NgoMIV 280 GCCGGC G^CCGGC
NspI 1030 ACATGC [GA]CATG^[CT]
PacI 2986 TTAATTAA TTAAT^TAA
PshBI 2559 ATTAAT AT^TAAT
PstI 575 CTGCAG CTGCA^G
PvuII 1686 CAGCTG CAG^CTG
RcaI 3180 TCATGA T^CATGA
SfoI 87 GGCGCC GGC^GCC
SmlI 515 CTCAAG C^T[CT][GA]AG
SnaBI 2592 TACGTA TAC^GTA
SspBI 3249 TGTACA T^GTACA
XbaI 3220 TCTAGA T^CTAGA
XcmI 2785 CCATACAAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1740 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 87 GGCGCC G[GA]^CG[CT]C
283 GGCGTC
AspHI 2090 GAGCAC G[AT]GC[AT]^C
3064 GTGCTC
AvaII 218 GGACC G^G[AT]CC
2512 GGACC
BsaHI 87 GGCGCC G[GA]^CG[CT]C
283 GGCGTC
BseRI 1794 GAGGAG GAGGAG(10/8)
2274 GAGGAG
BsiHKAI 2090 GAGCAC G[AT]GC[AT]^C
3064 GTGCTC
BsmI 1154 GCATTC GAATGC(1/-1)
1438 GCATTC
DraII 217 GGGACCC [GA]G^G[ACGT]CC[CT]
2511 GGGACCC
DsaI 178 CCGCGG C^C[GA][CT]GG
293 CCGCGG
EcoO109I 217 GGGACCC [GA]G^G[ACGT]CC[CT]
2511 GGGACCC
EcoT22I 2136 ATGCAT ATGCA^T
2950 ATGCAT
HgaI 1700 GACGC GACGC(5/10)
284 GCGTC
Hin1I 87 GGCGCC G[GA]^CG[CT]C
283 GGCGTC
HindIII 533 AAGCTT A^AGCTT
2603 AAGCTT
KspI 178 CCGCGG CCGC^GG
293 CCGCGG
NsiI 2136 ATGCAT ATGCA^T
2950 ATGCAT
PflMI 1259 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3083 CCATATTTTGG
PpuMI 217 GGGACCC [GA]G^G[AT]CC[CT]
2511 GGGACCC
SacII 178 CCGCGG CCGC^GG
293 CCGCGG
SfcI 575 CTGCAG C^T[GA][CT]AG
976 CTACAG
SmaI 68 CCCGGG CCC^GGG
96 CCCGGG
SpeI 2496 ACTAGT A^CTAGT
2943 ACTAGT
Van91I 1259 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3083 CCATATTTTGG
XmaCI 68 CCCGGG C^CCGGG
96 CCCGGG
XmaI 68 CCCGGG C^CCGGG
96 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI