##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
AocI ApaLI AscI AspEI AspI
AviII BalI BamHI BbrPI BcgI
BclI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsiWI BspEI BspHI
BsrBI BsrFI BstAPI BstEII BstPI
BstXI Bsu36I CelII Cfr10I CpoI
DraII DrdI Eam1105I Eco81I EcoNI
EcoO109I EcoO65I FbaI FseI FspI
HincII HindII HpaI MluNI MroI
MscI NaeI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI RcaI RsrII SalI
SbfI SfiI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 16 AACGTT AA^CGTT
AflII 3170 CTTAAG C^TTAAG
ApaI 40 GGGCCC GGGCC^C
AseI 1486 ATTAAT AT^TAAT
AsnI 1486 ATTAAT AT^TAAT
BbeI 573 GGCGCC GGCGC^C
BbvCI 1450 CCTCAGC CCTCAGC(-5/-2)
BfrI 3170 CTTAAG C^TTAAG
BglI 312 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 29 GTGCAG GTGCAG(16/14)
BsmBI 1020 GAGACG CGTCTC(1/5)
BsmFI 360 GTCCC GGGAC(10/14)
BsmI 2381 GCATTC GAATGC(1/-1)
Bsp1407I 3437 TGTACA T^GTACA
BspDI 2225 ATCGAT AT^CGAT
BspLU11I 2182 ACATGT A^CATGT
BsrDI 3185 GCAATG GCAATG(2/0)
BsrGI 3437 TGTACA T^GTACA
BssHII 249 GCGCGC G^CGCGC
BssSI 1704 CACGAG CACGAG(-5/-1)
Bst1107I 1474 GTATAC GTA^TAC
BstBI 2345 TTCGAA TT^CGAA
BstZ17I 1474 GTATAC GTA^TAC
ClaI 2225 ATCGAT AT^CGAT
DraIII 2386 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 216 CGGCCG C^GGCCG
EclXI 216 CGGCCG C^GGCCG
Eco52I 216 CGGCCG C^GGCCG
EcoRV 2222 GATATC GAT^ATC
HindIII 2711 AAGCTT A^AGCTT
KasI 573 GGCGCC G^GCGCC
MluI 1880 ACGCGT A^CGCGT
MslI 1549 CATCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 573 GGCGCC GG^CGCC
NspV 2345 TTCGAA TT^CGAA
PshBI 1486 ATTAAT AT^TAAT
Psp1406I 16 AACGTT AA^CGTT
PvuI 273 CGATCG CGAT^CG
SexAI 1606 ACCTGGT A^CC[AT]GGT
SfoI 573 GGCGCC GGC^GCC
SfuI 2345 TTCGAA TT^CGAA
SmlI 3170 CTTAAG C^T[CT][GA]AG
SpeI 1342 ACTAGT A^CTAGT
SspBI 3437 TGTACA T^GTACA
XcmI 3177 CCAGAACTGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 958 GGTACC G^GTACC
2602 GGTACC
AccI 919 GTCTAC GT^[AC][GT]AC
1474 GTATAC
AflIII 1880 ACGCGT A^C[GA][CT]GT
2182 ACATGT
Aor51HI 80 AGCGCT AGC^GCT
353 AGCGCT
Asp700I 2150 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2453 GAAGCTCTTC
Asp718I 958 GGTACC G^GTACC
2602 GGTACC
AvrII 136 CCTAGG C^CTAGG
829 CCTAGG
BciVI 2320 GGATAC GTATCC(6/5)
807 GTATCC
BglII 971 AGATCT A^GATCT
1960 AGATCT
BlnI 136 CCTAGG C^CTAGG
829 CCTAGG
BsiEI 216 CGGCCG CG[GA][CT]^CG
273 CGATCG
BspMI 526 ACCTGC ACCTGC(4/8)
1785 GCAGGT
DsaI 2 CCGCGG C^C[GA][CT]GG
213 CCGCGG
EaeI 216 CGGCCG [CT]^GGCC[GA]
348 CGGCCA
Eco47III 80 AGCGCT AGC^GCT
353 AGCGCT
KpnI 958 GGTACC GGTAC^C
2602 GGTACC
KspI 2 CCGCGG CCGC^GG
213 CCGCGG
MfeI 2734 CAATTG C^AATTG
3448 CAATTG
MunI 2734 CAATTG C^AATTG
3448 CAATTG
NspI 1761 GCATGT [GA]CATG^[CT]
2182 ACATGT
PstI 1783 CTGCAG CTGCA^G
2259 CTGCAG
SacI 2882 GAGCTC GAGCT^C
3191 GAGCTC
SacII 2 CCGCGG CCGC^GG
213 CCGCGG
ScaI 1708 AGTACT AGT^ACT
1779 AGTACT
SspI 385 AATATT AAT^ATT
2850 AATATT
StuI 133 AGGCCT AGG^CCT
139 AGGCCT
XmnI 2150 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2453 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI PleI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII